Increased FUN14 domain containing 1 (FUNDC1) ubiquitination level inhibits mitophagy and alleviates the injury in hypoxia-induced trophoblast cells

Preeclampsia (PE) is a pregnancy disorder characterized by excessive trophoblast cell death. This study aims to explore the exact mechanism of the ubiquitination level of FUN14 domain containing 1 (FUNDC1) in mitophagy and injury in hypoxic trophoblast cells. In this study, HTR-8/SVneo trophoblast c...

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Autores principales:	GuoQing Chen, Lu Chen, Yan Huang, XiongShan Zhu, YuanLan Yu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	preeclampsia ubiquitination fundc1 mitophagy trophoblast cells cell injury Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/e7b7debb99a64b45a4f2e00a99471e3c
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spelling	oai:doaj.org-article:e7b7debb99a64b45a4f2e00a99471e3c2021-11-04T15:51:54ZIncreased FUN14 domain containing 1 (FUNDC1) ubiquitination level inhibits mitophagy and alleviates the injury in hypoxia-induced trophoblast cells2165-59792165-598710.1080/21655979.2021.1997132https://doaj.org/article/e7b7debb99a64b45a4f2e00a99471e3c2021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1997132https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Preeclampsia (PE) is a pregnancy disorder characterized by excessive trophoblast cell death. This study aims to explore the exact mechanism of the ubiquitination level of FUN14 domain containing 1 (FUNDC1) in mitophagy and injury in hypoxic trophoblast cells. In this study, HTR-8/SVneo trophoblast cells were cultured under normoxic and hypoxic conditions and PE mouse model was established. We found low ubiquitination level of FUNDC1 in hypoxic trophoblast cells and placenta of pregnant women with PE. Proteasome inhibitor MG-132 and protease activator MF-094 were added into HTR-8/SVneo trophoblast cells. Proteasome inhibitor MG-132 decreased FUNDC1 ubiquitination level while protease activator MF-094 increased FUNDC1 ubiquitination level. Inhibition of FUNDC1 ubiquitination promoted mitophagy and mitochondrial membrane potential (Δψm) in normoxic trophoblast cells, increased levels of reactive oxygen species (ROS) and malondialdehyde (MDA) and decreased levels of glutathione (GSH) and superoxide dismutase (SOD). In addition, FUNDC1 ubiquitination alleviated cell injury in PE mice in vivo. In conclusion, increased FUNDC1 ubiquitination level inhibited mitophagy and Δψm changes in hypoxic trophoblast cells, and thus alleviated oxidative injury.GuoQing ChenLu ChenYan HuangXiongShan ZhuYuanLan YuTaylor & Francis Grouparticlepreeclampsiaubiquitinationfundc1mitophagytrophoblast cellscell injuryBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	preeclampsia ubiquitination fundc1 mitophagy trophoblast cells cell injury Biotechnology TP248.13-248.65
spellingShingle	preeclampsia ubiquitination fundc1 mitophagy trophoblast cells cell injury Biotechnology TP248.13-248.65 GuoQing Chen Lu Chen Yan Huang XiongShan Zhu YuanLan Yu Increased FUN14 domain containing 1 (FUNDC1) ubiquitination level inhibits mitophagy and alleviates the injury in hypoxia-induced trophoblast cells
description	Preeclampsia (PE) is a pregnancy disorder characterized by excessive trophoblast cell death. This study aims to explore the exact mechanism of the ubiquitination level of FUN14 domain containing 1 (FUNDC1) in mitophagy and injury in hypoxic trophoblast cells. In this study, HTR-8/SVneo trophoblast cells were cultured under normoxic and hypoxic conditions and PE mouse model was established. We found low ubiquitination level of FUNDC1 in hypoxic trophoblast cells and placenta of pregnant women with PE. Proteasome inhibitor MG-132 and protease activator MF-094 were added into HTR-8/SVneo trophoblast cells. Proteasome inhibitor MG-132 decreased FUNDC1 ubiquitination level while protease activator MF-094 increased FUNDC1 ubiquitination level. Inhibition of FUNDC1 ubiquitination promoted mitophagy and mitochondrial membrane potential (Δψm) in normoxic trophoblast cells, increased levels of reactive oxygen species (ROS) and malondialdehyde (MDA) and decreased levels of glutathione (GSH) and superoxide dismutase (SOD). In addition, FUNDC1 ubiquitination alleviated cell injury in PE mice in vivo. In conclusion, increased FUNDC1 ubiquitination level inhibited mitophagy and Δψm changes in hypoxic trophoblast cells, and thus alleviated oxidative injury.
format	article
author	GuoQing Chen Lu Chen Yan Huang XiongShan Zhu YuanLan Yu
author_facet	GuoQing Chen Lu Chen Yan Huang XiongShan Zhu YuanLan Yu
author_sort	GuoQing Chen
title	Increased FUN14 domain containing 1 (FUNDC1) ubiquitination level inhibits mitophagy and alleviates the injury in hypoxia-induced trophoblast cells
title_short	Increased FUN14 domain containing 1 (FUNDC1) ubiquitination level inhibits mitophagy and alleviates the injury in hypoxia-induced trophoblast cells
title_full	Increased FUN14 domain containing 1 (FUNDC1) ubiquitination level inhibits mitophagy and alleviates the injury in hypoxia-induced trophoblast cells
title_fullStr	Increased FUN14 domain containing 1 (FUNDC1) ubiquitination level inhibits mitophagy and alleviates the injury in hypoxia-induced trophoblast cells
title_full_unstemmed	Increased FUN14 domain containing 1 (FUNDC1) ubiquitination level inhibits mitophagy and alleviates the injury in hypoxia-induced trophoblast cells
title_sort	increased fun14 domain containing 1 (fundc1) ubiquitination level inhibits mitophagy and alleviates the injury in hypoxia-induced trophoblast cells
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/e7b7debb99a64b45a4f2e00a99471e3c
work_keys_str_mv	AT guoqingchen increasedfun14domaincontaining1fundc1ubiquitinationlevelinhibitsmitophagyandalleviatestheinjuryinhypoxiainducedtrophoblastcells AT luchen increasedfun14domaincontaining1fundc1ubiquitinationlevelinhibitsmitophagyandalleviatestheinjuryinhypoxiainducedtrophoblastcells AT yanhuang increasedfun14domaincontaining1fundc1ubiquitinationlevelinhibitsmitophagyandalleviatestheinjuryinhypoxiainducedtrophoblastcells AT xiongshanzhu increasedfun14domaincontaining1fundc1ubiquitinationlevelinhibitsmitophagyandalleviatestheinjuryinhypoxiainducedtrophoblastcells AT yuanlanyu increasedfun14domaincontaining1fundc1ubiquitinationlevelinhibitsmitophagyandalleviatestheinjuryinhypoxiainducedtrophoblastcells
_version_	1718444747415093248

Increased FUN14 domain containing 1 (FUNDC1) ubiquitination level inhibits mitophagy and alleviates the injury in hypoxia-induced trophoblast cells

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