An in-vitro assay using human spermatozoa to detect toxicity of biologically active substances

Abstract Identifying the key toxic players within an in-vivo toxic syndrome is crucial to develop targeted therapies. Here, we established a novel method that characterizes the effect of single substances by means of an ex-vivo incubation set-up. We found that primary human spermatozoa elicit a dist...

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Autores principales:	Tino Vollmer, Börje Ljungberg, Vera Jankowski, Joachim Jankowski, Griet Glorieux, Bernd G. Stegmayr
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e7c2ad1935834d62a724b3d954ef1964
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spelling	oai:doaj.org-article:e7c2ad1935834d62a724b3d954ef19642021-12-02T15:09:28ZAn in-vitro assay using human spermatozoa to detect toxicity of biologically active substances10.1038/s41598-019-50929-z2045-2322https://doaj.org/article/e7c2ad1935834d62a724b3d954ef19642019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-50929-zhttps://doaj.org/toc/2045-2322Abstract Identifying the key toxic players within an in-vivo toxic syndrome is crucial to develop targeted therapies. Here, we established a novel method that characterizes the effect of single substances by means of an ex-vivo incubation set-up. We found that primary human spermatozoa elicit a distinct motile response on a (uremic) toxic milieu. Specifically, this approach describes the influence of a bulk toxic environment (uremia) as well as single substances (uremic toxins) by real-time analyzing motile cellular behavior. We established the human spermatozoa-based toxicity testing (HSTT) for detecting single substance-induced toxicity to be used as a screening tool to identify in-vivo toxins. Further, we propose an application of the HSTT as a method of clinical use to evaluate toxin-removing interventions (hemodialysis).Tino VollmerBörje LjungbergVera JankowskiJoachim JankowskiGriet GlorieuxBernd G. StegmayrNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tino Vollmer Börje Ljungberg Vera Jankowski Joachim Jankowski Griet Glorieux Bernd G. Stegmayr An in-vitro assay using human spermatozoa to detect toxicity of biologically active substances
description	Abstract Identifying the key toxic players within an in-vivo toxic syndrome is crucial to develop targeted therapies. Here, we established a novel method that characterizes the effect of single substances by means of an ex-vivo incubation set-up. We found that primary human spermatozoa elicit a distinct motile response on a (uremic) toxic milieu. Specifically, this approach describes the influence of a bulk toxic environment (uremia) as well as single substances (uremic toxins) by real-time analyzing motile cellular behavior. We established the human spermatozoa-based toxicity testing (HSTT) for detecting single substance-induced toxicity to be used as a screening tool to identify in-vivo toxins. Further, we propose an application of the HSTT as a method of clinical use to evaluate toxin-removing interventions (hemodialysis).
format	article
author	Tino Vollmer Börje Ljungberg Vera Jankowski Joachim Jankowski Griet Glorieux Bernd G. Stegmayr
author_facet	Tino Vollmer Börje Ljungberg Vera Jankowski Joachim Jankowski Griet Glorieux Bernd G. Stegmayr
author_sort	Tino Vollmer
title	An in-vitro assay using human spermatozoa to detect toxicity of biologically active substances
title_short	An in-vitro assay using human spermatozoa to detect toxicity of biologically active substances
title_full	An in-vitro assay using human spermatozoa to detect toxicity of biologically active substances
title_fullStr	An in-vitro assay using human spermatozoa to detect toxicity of biologically active substances
title_full_unstemmed	An in-vitro assay using human spermatozoa to detect toxicity of biologically active substances
title_sort	in-vitro assay using human spermatozoa to detect toxicity of biologically active substances
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/e7c2ad1935834d62a724b3d954ef1964
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An in-vitro assay using human spermatozoa to detect toxicity of biologically active substances

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