Transcriptional profile of AvrRpt2 EA -mediated resistance and susceptibility response to Erwinia amylovora in apple

Abstract Most of the commercial apple cultivars are highly susceptible to fire blight, which is the most devastating bacterial disease affecting pome fruits. Resistance to fire blight is described especially in wild Malus accessions such as M. × robusta 5 (Mr5), but the molecular basis of host resis...

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Autores principales:	Susan Schröpfer, Isabelle Vogt, Giovanni Antonio Lodovico Broggini, Andreas Dahl, Klaus Richter, Magda-Viola Hanke, Henryk Flachowsky, Andreas Peil
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e7c508b50a5a460fb843e2b2669068e2
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spelling	oai:doaj.org-article:e7c508b50a5a460fb843e2b2669068e22021-12-02T16:45:31ZTranscriptional profile of AvrRpt2 EA -mediated resistance and susceptibility response to Erwinia amylovora in apple10.1038/s41598-021-88032-x2045-2322https://doaj.org/article/e7c508b50a5a460fb843e2b2669068e22021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88032-xhttps://doaj.org/toc/2045-2322Abstract Most of the commercial apple cultivars are highly susceptible to fire blight, which is the most devastating bacterial disease affecting pome fruits. Resistance to fire blight is described especially in wild Malus accessions such as M. × robusta 5 (Mr5), but the molecular basis of host resistance response to the pathogen Erwinia amylovora is still largely unknown. The bacterial effector protein AvrRpt2EA was found to be the key determinant of resistance response in Mr5. A wild type E. amylovora strain and the corresponding avrRpt2 EA deletion mutant were used for inoculation of Mr5 to induce resistance or susceptible response, respectively. By comparison of the transcriptome of both responses, 211 differentially expressed genes (DEGs) were identified. We found that heat-shock response including heat-shock proteins (HSPs) and heat-shock transcription factors (HSFs) are activated in apple specifically in the susceptible response, independent of AvrRpt2 EA . Further analysis on the expression progress of 81 DEGs by high-throughput real-time qPCR resulted in the identification of genes that were activated after inoculation with E. amylovora. Hence, a potential role of these genes in the resistance to the pathogen is postulated, including genes coding for enzymes involved in formation of flavonoids and terpenoids, ribosome-inactivating enzymes (RIPs) and a squamosa promoter binding-like (SPL) transcription factor.Susan SchröpferIsabelle VogtGiovanni Antonio Lodovico BrogginiAndreas DahlKlaus RichterMagda-Viola HankeHenryk FlachowskyAndreas PeilNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Susan Schröpfer Isabelle Vogt Giovanni Antonio Lodovico Broggini Andreas Dahl Klaus Richter Magda-Viola Hanke Henryk Flachowsky Andreas Peil Transcriptional profile of AvrRpt2 EA -mediated resistance and susceptibility response to Erwinia amylovora in apple
description	Abstract Most of the commercial apple cultivars are highly susceptible to fire blight, which is the most devastating bacterial disease affecting pome fruits. Resistance to fire blight is described especially in wild Malus accessions such as M. × robusta 5 (Mr5), but the molecular basis of host resistance response to the pathogen Erwinia amylovora is still largely unknown. The bacterial effector protein AvrRpt2EA was found to be the key determinant of resistance response in Mr5. A wild type E. amylovora strain and the corresponding avrRpt2 EA deletion mutant were used for inoculation of Mr5 to induce resistance or susceptible response, respectively. By comparison of the transcriptome of both responses, 211 differentially expressed genes (DEGs) were identified. We found that heat-shock response including heat-shock proteins (HSPs) and heat-shock transcription factors (HSFs) are activated in apple specifically in the susceptible response, independent of AvrRpt2 EA . Further analysis on the expression progress of 81 DEGs by high-throughput real-time qPCR resulted in the identification of genes that were activated after inoculation with E. amylovora. Hence, a potential role of these genes in the resistance to the pathogen is postulated, including genes coding for enzymes involved in formation of flavonoids and terpenoids, ribosome-inactivating enzymes (RIPs) and a squamosa promoter binding-like (SPL) transcription factor.
format	article
author	Susan Schröpfer Isabelle Vogt Giovanni Antonio Lodovico Broggini Andreas Dahl Klaus Richter Magda-Viola Hanke Henryk Flachowsky Andreas Peil
author_facet	Susan Schröpfer Isabelle Vogt Giovanni Antonio Lodovico Broggini Andreas Dahl Klaus Richter Magda-Viola Hanke Henryk Flachowsky Andreas Peil
author_sort	Susan Schröpfer
title	Transcriptional profile of AvrRpt2 EA -mediated resistance and susceptibility response to Erwinia amylovora in apple
title_short	Transcriptional profile of AvrRpt2 EA -mediated resistance and susceptibility response to Erwinia amylovora in apple
title_full	Transcriptional profile of AvrRpt2 EA -mediated resistance and susceptibility response to Erwinia amylovora in apple
title_fullStr	Transcriptional profile of AvrRpt2 EA -mediated resistance and susceptibility response to Erwinia amylovora in apple
title_full_unstemmed	Transcriptional profile of AvrRpt2 EA -mediated resistance and susceptibility response to Erwinia amylovora in apple
title_sort	transcriptional profile of avrrpt2 ea -mediated resistance and susceptibility response to erwinia amylovora in apple
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e7c508b50a5a460fb843e2b2669068e2
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Transcriptional profile of AvrRpt2 EA -mediated resistance and susceptibility response to Erwinia amylovora in apple

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