A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis.
Meiosis in angiosperm plants is followed by mitotic divisions to form multicellular haploid gametophytes. Termination of meiosis and transition to gametophytic development is, in Arabidopsis, governed by a dedicated mechanism that involves SMG7 and TDM1 proteins. Mutants carrying the smg7-6 allele a...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/e7cd73f985f246cab1f4184b3d27a750 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:e7cd73f985f246cab1f4184b3d27a750 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:e7cd73f985f246cab1f4184b3d27a7502021-12-02T20:03:01ZA CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis.1553-73901553-740410.1371/journal.pgen.1009779https://doaj.org/article/e7cd73f985f246cab1f4184b3d27a7502021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009779https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Meiosis in angiosperm plants is followed by mitotic divisions to form multicellular haploid gametophytes. Termination of meiosis and transition to gametophytic development is, in Arabidopsis, governed by a dedicated mechanism that involves SMG7 and TDM1 proteins. Mutants carrying the smg7-6 allele are semi-fertile due to reduced pollen production. We found that instead of forming tetrads, smg7-6 pollen mother cells undergo multiple rounds of chromosome condensation and spindle assembly at the end of meiosis, resembling aberrant attempts to undergo additional meiotic divisions. A suppressor screen uncovered a mutation in centromeric histone H3 (CENH3) that increased fertility and promoted meiotic exit in smg7-6 plants. The mutation led to inefficient splicing of the CENH3 mRNA and a substantial decrease of CENH3, resulting in smaller centromeres. The reduced level of CENH3 delayed formation of the mitotic spindle but did not have an apparent effect on plant growth and development. We suggest that impaired spindle re-assembly at the end of meiosis limits aberrant divisions in smg7-6 plants and promotes formation of tetrads and viable pollen. Furthermore, the mutant with reduced level of CENH3 was very inefficient haploid inducer indicating that differences in centromere size is not the key determinant of centromere-mediated genome elimination.Claudio CapitaoSorin TanasaJaroslav FulnecekVivek K RaxwalSvetlana AkimchevaPetra BulankovaPavlina MikulkovaLucie Crhak KhaitovaManikandan KalidassInna LermontovaOrtrun Mittelsten ScheidKarel RihaPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 9, p e1009779 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Genetics QH426-470 |
| spellingShingle |
Genetics QH426-470 Claudio Capitao Sorin Tanasa Jaroslav Fulnecek Vivek K Raxwal Svetlana Akimcheva Petra Bulankova Pavlina Mikulkova Lucie Crhak Khaitova Manikandan Kalidass Inna Lermontova Ortrun Mittelsten Scheid Karel Riha A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis. |
| description |
Meiosis in angiosperm plants is followed by mitotic divisions to form multicellular haploid gametophytes. Termination of meiosis and transition to gametophytic development is, in Arabidopsis, governed by a dedicated mechanism that involves SMG7 and TDM1 proteins. Mutants carrying the smg7-6 allele are semi-fertile due to reduced pollen production. We found that instead of forming tetrads, smg7-6 pollen mother cells undergo multiple rounds of chromosome condensation and spindle assembly at the end of meiosis, resembling aberrant attempts to undergo additional meiotic divisions. A suppressor screen uncovered a mutation in centromeric histone H3 (CENH3) that increased fertility and promoted meiotic exit in smg7-6 plants. The mutation led to inefficient splicing of the CENH3 mRNA and a substantial decrease of CENH3, resulting in smaller centromeres. The reduced level of CENH3 delayed formation of the mitotic spindle but did not have an apparent effect on plant growth and development. We suggest that impaired spindle re-assembly at the end of meiosis limits aberrant divisions in smg7-6 plants and promotes formation of tetrads and viable pollen. Furthermore, the mutant with reduced level of CENH3 was very inefficient haploid inducer indicating that differences in centromere size is not the key determinant of centromere-mediated genome elimination. |
| format |
article |
| author |
Claudio Capitao Sorin Tanasa Jaroslav Fulnecek Vivek K Raxwal Svetlana Akimcheva Petra Bulankova Pavlina Mikulkova Lucie Crhak Khaitova Manikandan Kalidass Inna Lermontova Ortrun Mittelsten Scheid Karel Riha |
| author_facet |
Claudio Capitao Sorin Tanasa Jaroslav Fulnecek Vivek K Raxwal Svetlana Akimcheva Petra Bulankova Pavlina Mikulkova Lucie Crhak Khaitova Manikandan Kalidass Inna Lermontova Ortrun Mittelsten Scheid Karel Riha |
| author_sort |
Claudio Capitao |
| title |
A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis. |
| title_short |
A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis. |
| title_full |
A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis. |
| title_fullStr |
A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis. |
| title_full_unstemmed |
A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis. |
| title_sort |
cenh3 mutation promotes meiotic exit and restores fertility in smg7-deficient arabidopsis. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/e7cd73f985f246cab1f4184b3d27a750 |
| work_keys_str_mv |
AT claudiocapitao acenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT sorintanasa acenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT jaroslavfulnecek acenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT vivekkraxwal acenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT svetlanaakimcheva acenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT petrabulankova acenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT pavlinamikulkova acenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT luciecrhakkhaitova acenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT manikandankalidass acenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT innalermontova acenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT ortrunmittelstenscheid acenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT karelriha acenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT claudiocapitao cenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT sorintanasa cenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT jaroslavfulnecek cenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT vivekkraxwal cenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT svetlanaakimcheva cenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT petrabulankova cenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT pavlinamikulkova cenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT luciecrhakkhaitova cenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT manikandankalidass cenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT innalermontova cenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT ortrunmittelstenscheid cenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis AT karelriha cenh3mutationpromotesmeioticexitandrestoresfertilityinsmg7deficientarabidopsis |
| _version_ |
1718375694785839104 |