Non-immunogenic dextran-coated superparamagnetic iron oxide nanoparticles: a biocompatible, size-tunable contrast agent for magnetic resonance imaging

Harald Unterweger,1,* Christina Janko,1,* Marc Schwarz,2 László Dézsi,3 Rudolf Urbanics,4 Jasmin Matuszak,1 Erik Őrfi,3 Tamás Fülöp,3 Tobias Bäuerle,2 János Szebeni,3,4 Clément Journé,5 Aldo R Boc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Unterweger H, Janko C, Schwarz M, Dézsi L, Urbanics R, Matuszak J, Őrfi E, Fülöp T, Bäuerle T, Szebeni J, Journé C, Boccaccini AR, Alexiou C, Lyer S, Cicha I
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Superparamagnetic iron oxide nanoparticles MRI hypersensitivity reaction SPION uptake hemocompatibility Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e7d4dde894244cb881bf6ebf3dcc92dc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e7d4dde894244cb881bf6ebf3dcc92dc
record_format	dspace
spelling	oai:doaj.org-article:e7d4dde894244cb881bf6ebf3dcc92dc2021-12-02T05:04:29ZNon-immunogenic dextran-coated superparamagnetic iron oxide nanoparticles: a biocompatible, size-tunable contrast agent for magnetic resonance imaging1178-2013https://doaj.org/article/e7d4dde894244cb881bf6ebf3dcc92dc2017-07-01T00:00:00Zhttps://www.dovepress.com/non-immunogenic-dextran-coated-superparamagnetic-iron-oxide-nanopartic-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Harald Unterweger,1,* Christina Janko,1,* Marc Schwarz,2 László Dézsi,3 Rudolf Urbanics,4 Jasmin Matuszak,1 Erik Őrfi,3 Tamás Fülöp,3 Tobias Bäuerle,2 János Szebeni,3,4 Clément Journé,5 Aldo R Boccaccini,6 Christoph Alexiou,1 Stefan Lyer,1 Iwona Cicha1 1Cardiovascular Nanomedicine Unit, Section of Experimental Oncology und Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, ENT Department, University Hospital Erlangen, Friedrich-Alexander-Universitaet Erlangen-Nuernberg, 2Preclinical Imaging Platform Erlangen (PIPE), Institute of Radiology, University Hospital Erlangen, Erlangen, Germany; 3Nanomedicine Research and Education Center, Semmelweis University, 4SeroScience Ltd., Budapest, Hungary; 5Inserm U1148, Fédération de Recherche en Imagerie Multimodalités (FRIM), X Bichat Hospital, Paris Diderot University, Paris, France; 6Institute of Biomaterials, Department of Materials Science and Engineering, University Erlangen-Nuremberg, Erlangen, Germany *These authors contributed equally to this work Abstract: Iron oxide-based contrast agents have been in clinical use for magnetic resonance imaging (MRI) of lymph nodes, liver, intestines, and the cardiovascular system. Superparamagnetic iron oxide nanoparticles (SPIONs) have high potential as a contrast agent for MRI, but no intravenous iron oxide-containing agents are currently approved for clinical imaging. The aim of our work was to analyze the hemocompatibility and immuno-safety of a new type of dextran-coated SPIONs (SPIONdex) and to characterize these nanoparticles with ultra-high-field MRI. Key parameters related to nanoparticle hemocompatibility and immuno-safety were investigated in vitro and ex vivo. To address concerns associated with hypersensitivity reactions to injectable nanoparticulate agents, we analyzed complement activation-related pseudoallergy (CARPA) upon intravenous administration of SPIONdex in a pig model. Furthermore, the size-tunability of SPIONdex and the effects of size reduction on their biocompatibility were investigated. In vitro, SPIONdex did not induce hemolysis, complement or platelet activation, plasma coagulation, or leukocyte procoagulant activity, and had no relevant effect on endothelial cell viability or endothelial–monocytic cell interactions. Furthermore, SPIONdex did not induce CARPA even upon intravenous administration of 5 mg Fe/kg in pigs. Upon SPIONdex administration in mice, decreased liver signal intensity was observed after 15 minutes and was still detectable 24 h later. In addition, by changing synthesis parameters, a reduction in particle size <30 nm was achieved, without affecting their hemo- and biocompatibility. Our findings suggest that due to their excellent biocompatibility, safety upon intravenous administration and size-tunability, SPIONdex particles may represent a suitable candidate for a new-generation MRI contrast agent. Keywords: superparamagnetic iron oxide nanoparticles, MRI, hypersensitivity reaction, SPION uptake, hemocompatibilityUnterweger HJanko CSchwarz MDézsi LUrbanics RMatuszak JŐrfi EFülöp TBäuerle TSzebeni JJourné CBoccaccini ARAlexiou CLyer SCicha IDove Medical PressarticleSuperparamagnetic iron oxide nanoparticlesMRIhypersensitivity reactionSPION uptakehemocompatibilityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 5223-5238 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Superparamagnetic iron oxide nanoparticles MRI hypersensitivity reaction SPION uptake hemocompatibility Medicine (General) R5-920
spellingShingle	Superparamagnetic iron oxide nanoparticles MRI hypersensitivity reaction SPION uptake hemocompatibility Medicine (General) R5-920 Unterweger H Janko C Schwarz M Dézsi L Urbanics R Matuszak J Őrfi E Fülöp T Bäuerle T Szebeni J Journé C Boccaccini AR Alexiou C Lyer S Cicha I Non-immunogenic dextran-coated superparamagnetic iron oxide nanoparticles: a biocompatible, size-tunable contrast agent for magnetic resonance imaging
description	Harald Unterweger,1,* Christina Janko,1,* Marc Schwarz,2 László Dézsi,3 Rudolf Urbanics,4 Jasmin Matuszak,1 Erik Őrfi,3 Tamás Fülöp,3 Tobias Bäuerle,2 János Szebeni,3,4 Clément Journé,5 Aldo R Boccaccini,6 Christoph Alexiou,1 Stefan Lyer,1 Iwona Cicha1 1Cardiovascular Nanomedicine Unit, Section of Experimental Oncology und Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, ENT Department, University Hospital Erlangen, Friedrich-Alexander-Universitaet Erlangen-Nuernberg, 2Preclinical Imaging Platform Erlangen (PIPE), Institute of Radiology, University Hospital Erlangen, Erlangen, Germany; 3Nanomedicine Research and Education Center, Semmelweis University, 4SeroScience Ltd., Budapest, Hungary; 5Inserm U1148, Fédération de Recherche en Imagerie Multimodalités (FRIM), X Bichat Hospital, Paris Diderot University, Paris, France; 6Institute of Biomaterials, Department of Materials Science and Engineering, University Erlangen-Nuremberg, Erlangen, Germany *These authors contributed equally to this work Abstract: Iron oxide-based contrast agents have been in clinical use for magnetic resonance imaging (MRI) of lymph nodes, liver, intestines, and the cardiovascular system. Superparamagnetic iron oxide nanoparticles (SPIONs) have high potential as a contrast agent for MRI, but no intravenous iron oxide-containing agents are currently approved for clinical imaging. The aim of our work was to analyze the hemocompatibility and immuno-safety of a new type of dextran-coated SPIONs (SPIONdex) and to characterize these nanoparticles with ultra-high-field MRI. Key parameters related to nanoparticle hemocompatibility and immuno-safety were investigated in vitro and ex vivo. To address concerns associated with hypersensitivity reactions to injectable nanoparticulate agents, we analyzed complement activation-related pseudoallergy (CARPA) upon intravenous administration of SPIONdex in a pig model. Furthermore, the size-tunability of SPIONdex and the effects of size reduction on their biocompatibility were investigated. In vitro, SPIONdex did not induce hemolysis, complement or platelet activation, plasma coagulation, or leukocyte procoagulant activity, and had no relevant effect on endothelial cell viability or endothelial–monocytic cell interactions. Furthermore, SPIONdex did not induce CARPA even upon intravenous administration of 5 mg Fe/kg in pigs. Upon SPIONdex administration in mice, decreased liver signal intensity was observed after 15 minutes and was still detectable 24 h later. In addition, by changing synthesis parameters, a reduction in particle size <30 nm was achieved, without affecting their hemo- and biocompatibility. Our findings suggest that due to their excellent biocompatibility, safety upon intravenous administration and size-tunability, SPIONdex particles may represent a suitable candidate for a new-generation MRI contrast agent. Keywords: superparamagnetic iron oxide nanoparticles, MRI, hypersensitivity reaction, SPION uptake, hemocompatibility
format	article
author	Unterweger H Janko C Schwarz M Dézsi L Urbanics R Matuszak J Őrfi E Fülöp T Bäuerle T Szebeni J Journé C Boccaccini AR Alexiou C Lyer S Cicha I
author_facet	Unterweger H Janko C Schwarz M Dézsi L Urbanics R Matuszak J Őrfi E Fülöp T Bäuerle T Szebeni J Journé C Boccaccini AR Alexiou C Lyer S Cicha I
author_sort	Unterweger H
title	Non-immunogenic dextran-coated superparamagnetic iron oxide nanoparticles: a biocompatible, size-tunable contrast agent for magnetic resonance imaging
title_short	Non-immunogenic dextran-coated superparamagnetic iron oxide nanoparticles: a biocompatible, size-tunable contrast agent for magnetic resonance imaging
title_full	Non-immunogenic dextran-coated superparamagnetic iron oxide nanoparticles: a biocompatible, size-tunable contrast agent for magnetic resonance imaging
title_fullStr	Non-immunogenic dextran-coated superparamagnetic iron oxide nanoparticles: a biocompatible, size-tunable contrast agent for magnetic resonance imaging
title_full_unstemmed	Non-immunogenic dextran-coated superparamagnetic iron oxide nanoparticles: a biocompatible, size-tunable contrast agent for magnetic resonance imaging
title_sort	non-immunogenic dextran-coated superparamagnetic iron oxide nanoparticles: a biocompatible, size-tunable contrast agent for magnetic resonance imaging
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/e7d4dde894244cb881bf6ebf3dcc92dc
work_keys_str_mv	AT unterwegerh nonimmunogenicdextrancoatedsuperparamagneticironoxidenanoparticlesabiocompatiblesizetunablecontrastagentformagneticresonanceimaging AT jankoc nonimmunogenicdextrancoatedsuperparamagneticironoxidenanoparticlesabiocompatiblesizetunablecontrastagentformagneticresonanceimaging AT schwarzm nonimmunogenicdextrancoatedsuperparamagneticironoxidenanoparticlesabiocompatiblesizetunablecontrastagentformagneticresonanceimaging AT dezsil nonimmunogenicdextrancoatedsuperparamagneticironoxidenanoparticlesabiocompatiblesizetunablecontrastagentformagneticresonanceimaging AT urbanicsr nonimmunogenicdextrancoatedsuperparamagneticironoxidenanoparticlesabiocompatiblesizetunablecontrastagentformagneticresonanceimaging AT matuszakj nonimmunogenicdextrancoatedsuperparamagneticironoxidenanoparticlesabiocompatiblesizetunablecontrastagentformagneticresonanceimaging AT orfie nonimmunogenicdextrancoatedsuperparamagneticironoxidenanoparticlesabiocompatiblesizetunablecontrastagentformagneticresonanceimaging AT fulopt nonimmunogenicdextrancoatedsuperparamagneticironoxidenanoparticlesabiocompatiblesizetunablecontrastagentformagneticresonanceimaging AT bauerlet nonimmunogenicdextrancoatedsuperparamagneticironoxidenanoparticlesabiocompatiblesizetunablecontrastagentformagneticresonanceimaging AT szebenij nonimmunogenicdextrancoatedsuperparamagneticironoxidenanoparticlesabiocompatiblesizetunablecontrastagentformagneticresonanceimaging AT journec nonimmunogenicdextrancoatedsuperparamagneticironoxidenanoparticlesabiocompatiblesizetunablecontrastagentformagneticresonanceimaging AT boccacciniar nonimmunogenicdextrancoatedsuperparamagneticironoxidenanoparticlesabiocompatiblesizetunablecontrastagentformagneticresonanceimaging AT alexiouc nonimmunogenicdextrancoatedsuperparamagneticironoxidenanoparticlesabiocompatiblesizetunablecontrastagentformagneticresonanceimaging AT lyers nonimmunogenicdextrancoatedsuperparamagneticironoxidenanoparticlesabiocompatiblesizetunablecontrastagentformagneticresonanceimaging AT cichai nonimmunogenicdextrancoatedsuperparamagneticironoxidenanoparticlesabiocompatiblesizetunablecontrastagentformagneticresonanceimaging
_version_	1718400684403982336

Non-immunogenic dextran-coated superparamagnetic iron oxide nanoparticles: a biocompatible, size-tunable contrast agent for magnetic resonance imaging

Ejemplares similares