UVC disinfects SARS-CoV-2 by induction of viral genome damage without apparent effects on viral morphology and proteins

Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been a pandemic threat worldwide and causes severe health and economic burdens. Contaminated environments, such as personal items and room surfaces, are considered to have virus transmission potential. Ultraviolet C (UVC) ligh...

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Autores principales:	Chieh-Wen Lo, Ryosuke Matsuura, Kazuki Iimura, Satoshi Wada, Atsushi Shinjo, Yoshimi Benno, Masaru Nakagawa, Masami Takei, Yoko Aida
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e7d91fbd69a445bc874e86d66d674812
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spelling	oai:doaj.org-article:e7d91fbd69a445bc874e86d66d6748122021-12-02T18:34:13ZUVC disinfects SARS-CoV-2 by induction of viral genome damage without apparent effects on viral morphology and proteins10.1038/s41598-021-93231-72045-2322https://doaj.org/article/e7d91fbd69a445bc874e86d66d6748122021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93231-7https://doaj.org/toc/2045-2322Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been a pandemic threat worldwide and causes severe health and economic burdens. Contaminated environments, such as personal items and room surfaces, are considered to have virus transmission potential. Ultraviolet C (UVC) light has demonstrated germicidal ability and removes environmental contamination. UVC has inactivated SARS-CoV-2; however, the underlying mechanisms are not clear. It was confirmed here that UVC 253.7 nm, with a dose of 500 μW/cm2, completely inactivated SARS-CoV-2 in a time-dependent manner and reduced virus infectivity by 10–4.9-fold within 30 s. Immunoblotting analysis for viral spike and nucleocapsid proteins showed that UVC treatment did not damage viral proteins. The viral particle morphology remained intact even when the virus completely lost infectivity after UVC irradiation, as observed by transmission electronic microscopy. In contrast, UVC irradiation-induced genome damage was identified using the newly developed long reverse-transcription quantitative-polymerase chain reaction (RT-qPCR) assay, but not conventional RT-qPCR. The six developed long RT-PCR assays that covered the full-length viral genome clearly indicated a negative correlation between virus infectivity and UVC irradiation-induced genome damage (R2 ranging from 0.75 to 0.96). Altogether, these results provide evidence that UVC inactivates SARS-CoV-2 through the induction of viral genome damage.Chieh-Wen LoRyosuke MatsuuraKazuki IimuraSatoshi WadaAtsushi ShinjoYoshimi BennoMasaru NakagawaMasami TakeiYoko AidaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chieh-Wen Lo Ryosuke Matsuura Kazuki Iimura Satoshi Wada Atsushi Shinjo Yoshimi Benno Masaru Nakagawa Masami Takei Yoko Aida UVC disinfects SARS-CoV-2 by induction of viral genome damage without apparent effects on viral morphology and proteins
description	Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been a pandemic threat worldwide and causes severe health and economic burdens. Contaminated environments, such as personal items and room surfaces, are considered to have virus transmission potential. Ultraviolet C (UVC) light has demonstrated germicidal ability and removes environmental contamination. UVC has inactivated SARS-CoV-2; however, the underlying mechanisms are not clear. It was confirmed here that UVC 253.7 nm, with a dose of 500 μW/cm2, completely inactivated SARS-CoV-2 in a time-dependent manner and reduced virus infectivity by 10–4.9-fold within 30 s. Immunoblotting analysis for viral spike and nucleocapsid proteins showed that UVC treatment did not damage viral proteins. The viral particle morphology remained intact even when the virus completely lost infectivity after UVC irradiation, as observed by transmission electronic microscopy. In contrast, UVC irradiation-induced genome damage was identified using the newly developed long reverse-transcription quantitative-polymerase chain reaction (RT-qPCR) assay, but not conventional RT-qPCR. The six developed long RT-PCR assays that covered the full-length viral genome clearly indicated a negative correlation between virus infectivity and UVC irradiation-induced genome damage (R2 ranging from 0.75 to 0.96). Altogether, these results provide evidence that UVC inactivates SARS-CoV-2 through the induction of viral genome damage.
format	article
author	Chieh-Wen Lo Ryosuke Matsuura Kazuki Iimura Satoshi Wada Atsushi Shinjo Yoshimi Benno Masaru Nakagawa Masami Takei Yoko Aida
author_facet	Chieh-Wen Lo Ryosuke Matsuura Kazuki Iimura Satoshi Wada Atsushi Shinjo Yoshimi Benno Masaru Nakagawa Masami Takei Yoko Aida
author_sort	Chieh-Wen Lo
title	UVC disinfects SARS-CoV-2 by induction of viral genome damage without apparent effects on viral morphology and proteins
title_short	UVC disinfects SARS-CoV-2 by induction of viral genome damage without apparent effects on viral morphology and proteins
title_full	UVC disinfects SARS-CoV-2 by induction of viral genome damage without apparent effects on viral morphology and proteins
title_fullStr	UVC disinfects SARS-CoV-2 by induction of viral genome damage without apparent effects on viral morphology and proteins
title_full_unstemmed	UVC disinfects SARS-CoV-2 by induction of viral genome damage without apparent effects on viral morphology and proteins
title_sort	uvc disinfects sars-cov-2 by induction of viral genome damage without apparent effects on viral morphology and proteins
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e7d91fbd69a445bc874e86d66d674812
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UVC disinfects SARS-CoV-2 by induction of viral genome damage without apparent effects on viral morphology and proteins

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