Discovery and characterization of human exonic transcriptional regulatory elements.

We sought exonic transcriptional regulatory elements by shotgun cloning human cDNA fragments into luciferase reporter vectors and measuring the resulting expression levels in liver cells. We uncovered seven regulatory elements within coding regions and three within 3' untranslated regions (UTRs...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Arshad H Khan, Andy Lin, Desmond J Smith
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e7ddf6fd1694459392de2c899988137d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e7ddf6fd1694459392de2c899988137d
record_format	dspace
spelling	oai:doaj.org-article:e7ddf6fd1694459392de2c899988137d2021-11-18T07:04:15ZDiscovery and characterization of human exonic transcriptional regulatory elements.1932-620310.1371/journal.pone.0046098https://doaj.org/article/e7ddf6fd1694459392de2c899988137d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23029400/?tool=EBIhttps://doaj.org/toc/1932-6203We sought exonic transcriptional regulatory elements by shotgun cloning human cDNA fragments into luciferase reporter vectors and measuring the resulting expression levels in liver cells. We uncovered seven regulatory elements within coding regions and three within 3' untranslated regions (UTRs). Two of the putative regulatory elements were enhancers and eight were silencers. The regulatory elements were generally but not consistently evolutionarily conserved and also showed a trend toward decreased population diversity. Furthermore, the exonic regulatory elements were enriched in known transcription factor binding sites (TFBSs) and were associated with several histone modifications and transcriptionally relevant chromatin. Evidence was obtained for bidirectional cis-regulation of a coding region element within a tubulin gene, TUBA1B, by the transcription factors PPARA and RORA. We estimate that hundreds of exonic transcriptional regulatory elements exist, an unexpected finding that highlights a surprising multi-functionality of sequences in the human genome.Arshad H KhanAndy LinDesmond J SmithPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e46098 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arshad H Khan Andy Lin Desmond J Smith Discovery and characterization of human exonic transcriptional regulatory elements.
description	We sought exonic transcriptional regulatory elements by shotgun cloning human cDNA fragments into luciferase reporter vectors and measuring the resulting expression levels in liver cells. We uncovered seven regulatory elements within coding regions and three within 3' untranslated regions (UTRs). Two of the putative regulatory elements were enhancers and eight were silencers. The regulatory elements were generally but not consistently evolutionarily conserved and also showed a trend toward decreased population diversity. Furthermore, the exonic regulatory elements were enriched in known transcription factor binding sites (TFBSs) and were associated with several histone modifications and transcriptionally relevant chromatin. Evidence was obtained for bidirectional cis-regulation of a coding region element within a tubulin gene, TUBA1B, by the transcription factors PPARA and RORA. We estimate that hundreds of exonic transcriptional regulatory elements exist, an unexpected finding that highlights a surprising multi-functionality of sequences in the human genome.
format	article
author	Arshad H Khan Andy Lin Desmond J Smith
author_facet	Arshad H Khan Andy Lin Desmond J Smith
author_sort	Arshad H Khan
title	Discovery and characterization of human exonic transcriptional regulatory elements.
title_short	Discovery and characterization of human exonic transcriptional regulatory elements.
title_full	Discovery and characterization of human exonic transcriptional regulatory elements.
title_fullStr	Discovery and characterization of human exonic transcriptional regulatory elements.
title_full_unstemmed	Discovery and characterization of human exonic transcriptional regulatory elements.
title_sort	discovery and characterization of human exonic transcriptional regulatory elements.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e7ddf6fd1694459392de2c899988137d
work_keys_str_mv	AT arshadhkhan discoveryandcharacterizationofhumanexonictranscriptionalregulatoryelements AT andylin discoveryandcharacterizationofhumanexonictranscriptionalregulatoryelements AT desmondjsmith discoveryandcharacterizationofhumanexonictranscriptionalregulatoryelements
_version_	1718424012476907520

Discovery and characterization of human exonic transcriptional regulatory elements.

Ejemplares similares