Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers

Abstract Conventional two-photon microscopes use photomultiplier tubes, which enable high sensitivity but can detect relatively few photons per second, forcing longer pixel integration times and limiting maximum imaging rates. We introduce novel detection electronics using silicon photomultipliers t...

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Autores principales:	Vincent D. Ching-Roa, Eben M. Olson, Sherrif F. Ibrahim, Richard Torres, Michael G. Giacomelli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e7deef1b6b114f769527cbff79fb3da8
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spelling	oai:doaj.org-article:e7deef1b6b114f769527cbff79fb3da82021-12-02T13:33:45ZUltrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers10.1038/s41598-021-84522-02045-2322https://doaj.org/article/e7deef1b6b114f769527cbff79fb3da82021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84522-0https://doaj.org/toc/2045-2322Abstract Conventional two-photon microscopes use photomultiplier tubes, which enable high sensitivity but can detect relatively few photons per second, forcing longer pixel integration times and limiting maximum imaging rates. We introduce novel detection electronics using silicon photomultipliers that greatly extend dynamic range, enabling more than an order of magnitude increased photon detection rate as compared to state-of-the-art photomultiplier tubes. We demonstrate that this capability can dramatically improve both imaging rates and signal-to-noise ratio (SNR) in two-photon microscopy using human surgical specimens. Finally, to enable wider use of more advanced detection technology, we have formed the OpenSiPM project, which aims to provide open source detector designs for high-speed two-photon and confocal microscopy.Vincent D. Ching-RoaEben M. OlsonSherrif F. IbrahimRichard TorresMichael G. GiacomelliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vincent D. Ching-Roa Eben M. Olson Sherrif F. Ibrahim Richard Torres Michael G. Giacomelli Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers
description	Abstract Conventional two-photon microscopes use photomultiplier tubes, which enable high sensitivity but can detect relatively few photons per second, forcing longer pixel integration times and limiting maximum imaging rates. We introduce novel detection electronics using silicon photomultipliers that greatly extend dynamic range, enabling more than an order of magnitude increased photon detection rate as compared to state-of-the-art photomultiplier tubes. We demonstrate that this capability can dramatically improve both imaging rates and signal-to-noise ratio (SNR) in two-photon microscopy using human surgical specimens. Finally, to enable wider use of more advanced detection technology, we have formed the OpenSiPM project, which aims to provide open source detector designs for high-speed two-photon and confocal microscopy.
format	article
author	Vincent D. Ching-Roa Eben M. Olson Sherrif F. Ibrahim Richard Torres Michael G. Giacomelli
author_facet	Vincent D. Ching-Roa Eben M. Olson Sherrif F. Ibrahim Richard Torres Michael G. Giacomelli
author_sort	Vincent D. Ching-Roa
title	Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers
title_short	Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers
title_full	Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers
title_fullStr	Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers
title_full_unstemmed	Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers
title_sort	ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e7deef1b6b114f769527cbff79fb3da8
work_keys_str_mv	AT vincentdchingroa ultrahighspeedpointscanningtwophotonmicroscopyusinghighdynamicrangesiliconphotomultipliers AT ebenmolson ultrahighspeedpointscanningtwophotonmicroscopyusinghighdynamicrangesiliconphotomultipliers AT sherriffibrahim ultrahighspeedpointscanningtwophotonmicroscopyusinghighdynamicrangesiliconphotomultipliers AT richardtorres ultrahighspeedpointscanningtwophotonmicroscopyusinghighdynamicrangesiliconphotomultipliers AT michaelggiacomelli ultrahighspeedpointscanningtwophotonmicroscopyusinghighdynamicrangesiliconphotomultipliers
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Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers

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