Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method

In the past few years, we have demonstrated the efficacy of a nanoparticle system, super carbonate apatite (sCA), for the in vivo delivery of siRNA/miRNA. Intravenous injection of sCA loaded with small RNAs results in safe, high tumor delivery in mouse models. To further improve the efficiency of tu...

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Autores principales:	Xin Wu, Yuhki Yokoyama, Hidekazu Takahashi, Shihori Kouda, Hiroyuki Yamamoto, Jiaqi Wang, Yoshihiro Morimoto, Kazumasa Minami, Tsuyoshi Hata, Awad Shamma, Akira Inoue, Masahisa Ohtsuka, Satoshi Shibata, Shogo Kobayashi, Shuji Akai, Hirofumi Yamamoto
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	iNaD siRNA microRNA calcium phosphate PEG blending cancer treatment Medicine R
Acceso en línea:	https://doaj.org/article/e7e544c360f24339ba9edac8c7656425
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spelling	oai:doaj.org-article:e7e544c360f24339ba9edac8c76564252021-11-25T18:07:38ZImproved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method10.3390/jpm111111602075-4426https://doaj.org/article/e7e544c360f24339ba9edac8c76564252021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4426/11/11/1160https://doaj.org/toc/2075-4426In the past few years, we have demonstrated the efficacy of a nanoparticle system, super carbonate apatite (sCA), for the in vivo delivery of siRNA/miRNA. Intravenous injection of sCA loaded with small RNAs results in safe, high tumor delivery in mouse models. To further improve the efficiency of tumor delivery and avoid liver toxicity, we successfully developed an inorganic nanoparticle device (iNaD) via high-frequency ultrasonic pulverization combined with PEG blending during the production of sCA. Compared to sCA loaded with 24 μg of miRNA, systemic administration of iNaD loaded with 0.75 μg of miRNA demonstrated similar delivery efficiency to mouse tumors with little accumulation in the liver. In the mouse therapeutic model, iNaD loaded with 3 μg of the tumor suppressor small RNA MIRTX resulted in an improved anti-tumor effect compared to sCA loaded with 24 μg. Our findings on the bio-distribution and therapeutic effect of iNaD provide new perspectives for future nanomedicine engineering.Xin WuYuhki YokoyamaHidekazu TakahashiShihori KoudaHiroyuki YamamotoJiaqi WangYoshihiro MorimotoKazumasa MinamiTsuyoshi HataAwad ShammaAkira InoueMasahisa OhtsukaSatoshi ShibataShogo KobayashiShuji AkaiHirofumi YamamotoMDPI AGarticleiNaDsiRNAmicroRNAcalcium phosphatePEG blendingcancer treatmentMedicineRENJournal of Personalized Medicine, Vol 11, Iss 1160, p 1160 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	iNaD siRNA microRNA calcium phosphate PEG blending cancer treatment Medicine R
spellingShingle	iNaD siRNA microRNA calcium phosphate PEG blending cancer treatment Medicine R Xin Wu Yuhki Yokoyama Hidekazu Takahashi Shihori Kouda Hiroyuki Yamamoto Jiaqi Wang Yoshihiro Morimoto Kazumasa Minami Tsuyoshi Hata Awad Shamma Akira Inoue Masahisa Ohtsuka Satoshi Shibata Shogo Kobayashi Shuji Akai Hirofumi Yamamoto Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method
description	In the past few years, we have demonstrated the efficacy of a nanoparticle system, super carbonate apatite (sCA), for the in vivo delivery of siRNA/miRNA. Intravenous injection of sCA loaded with small RNAs results in safe, high tumor delivery in mouse models. To further improve the efficiency of tumor delivery and avoid liver toxicity, we successfully developed an inorganic nanoparticle device (iNaD) via high-frequency ultrasonic pulverization combined with PEG blending during the production of sCA. Compared to sCA loaded with 24 μg of miRNA, systemic administration of iNaD loaded with 0.75 μg of miRNA demonstrated similar delivery efficiency to mouse tumors with little accumulation in the liver. In the mouse therapeutic model, iNaD loaded with 3 μg of the tumor suppressor small RNA MIRTX resulted in an improved anti-tumor effect compared to sCA loaded with 24 μg. Our findings on the bio-distribution and therapeutic effect of iNaD provide new perspectives for future nanomedicine engineering.
format	article
author	Xin Wu Yuhki Yokoyama Hidekazu Takahashi Shihori Kouda Hiroyuki Yamamoto Jiaqi Wang Yoshihiro Morimoto Kazumasa Minami Tsuyoshi Hata Awad Shamma Akira Inoue Masahisa Ohtsuka Satoshi Shibata Shogo Kobayashi Shuji Akai Hirofumi Yamamoto
author_facet	Xin Wu Yuhki Yokoyama Hidekazu Takahashi Shihori Kouda Hiroyuki Yamamoto Jiaqi Wang Yoshihiro Morimoto Kazumasa Minami Tsuyoshi Hata Awad Shamma Akira Inoue Masahisa Ohtsuka Satoshi Shibata Shogo Kobayashi Shuji Akai Hirofumi Yamamoto
author_sort	Xin Wu
title	Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method
title_short	Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method
title_full	Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method
title_fullStr	Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method
title_full_unstemmed	Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method
title_sort	improved in vivo delivery of small rna based on the calcium phosphate method
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e7e544c360f24339ba9edac8c7656425
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Improved In Vivo Delivery of Small RNA Based on the Calcium Phosphate Method

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