Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro

Abstract Platelet-rich fibrin (PRF) promotes wound healing by providing the release of growth factors. Here, the influence of Thai and Murrah bubaline blood derived PRF on canine periodontal ligament cells (cPDLs) was investigated. PRF was prepared from Thai and Murrah buffaloes with single centrifu...

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Autores principales:	Poranee Banyatworakul, Thanaphum Osathanon, Sujin Chumprasert, Prasit Pavasant, Nopadon Pirarat
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e7e8f6d8164e4868b5db20f03b5b2414
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spelling	oai:doaj.org-article:e7e8f6d8164e4868b5db20f03b5b24142021-12-02T15:02:31ZResponses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro10.1038/s41598-021-90906-z2045-2322https://doaj.org/article/e7e8f6d8164e4868b5db20f03b5b24142021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90906-zhttps://doaj.org/toc/2045-2322Abstract Platelet-rich fibrin (PRF) promotes wound healing by providing the release of growth factors. Here, the influence of Thai and Murrah bubaline blood derived PRF on canine periodontal ligament cells (cPDLs) was investigated. PRF was prepared from Thai and Murrah buffaloes with single centrifugation. Results demonstrated that Thai bubaline blood derived PRF exhibited fiber-mesh like morphology and contained more platelet entrapment than Murrah bubaline blood derived PRF. Both bubaline PRFs were able to degrade in vitro under condition with trypsin. Thai but not Murrah bubaline blood derived PRF promoted cPDLs proliferation in serum free and 2% serum culture conditions. Correspondingly, the significant upregulation of KI67 mRNA expression was observed in those cells treated with Thai bubaline blood derived PRF. However, both Thai and Murrah bubaline blood derived PRF accelerated cell migration in an in vitro wound healing assay and facilitated cell spreading. Further, cPDLs cultured in osteogenic induction medium supplemented with Thai bubaline blood derived PRF exhibited the increased mineral deposition in vitro. Frozen Thai bubaline blood derived PRF also promoted cell proliferation, KI67 mRNA expression, cell migration, and cell spreading in cPDLs. Taken these evidence together, bubaline blood derived PRF could provide potential benefits for canine periodontal tissue healing.Poranee BanyatworakulThanaphum OsathanonSujin ChumprasertPrasit PavasantNopadon PiraratNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Poranee Banyatworakul Thanaphum Osathanon Sujin Chumprasert Prasit Pavasant Nopadon Pirarat Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro
description	Abstract Platelet-rich fibrin (PRF) promotes wound healing by providing the release of growth factors. Here, the influence of Thai and Murrah bubaline blood derived PRF on canine periodontal ligament cells (cPDLs) was investigated. PRF was prepared from Thai and Murrah buffaloes with single centrifugation. Results demonstrated that Thai bubaline blood derived PRF exhibited fiber-mesh like morphology and contained more platelet entrapment than Murrah bubaline blood derived PRF. Both bubaline PRFs were able to degrade in vitro under condition with trypsin. Thai but not Murrah bubaline blood derived PRF promoted cPDLs proliferation in serum free and 2% serum culture conditions. Correspondingly, the significant upregulation of KI67 mRNA expression was observed in those cells treated with Thai bubaline blood derived PRF. However, both Thai and Murrah bubaline blood derived PRF accelerated cell migration in an in vitro wound healing assay and facilitated cell spreading. Further, cPDLs cultured in osteogenic induction medium supplemented with Thai bubaline blood derived PRF exhibited the increased mineral deposition in vitro. Frozen Thai bubaline blood derived PRF also promoted cell proliferation, KI67 mRNA expression, cell migration, and cell spreading in cPDLs. Taken these evidence together, bubaline blood derived PRF could provide potential benefits for canine periodontal tissue healing.
format	article
author	Poranee Banyatworakul Thanaphum Osathanon Sujin Chumprasert Prasit Pavasant Nopadon Pirarat
author_facet	Poranee Banyatworakul Thanaphum Osathanon Sujin Chumprasert Prasit Pavasant Nopadon Pirarat
author_sort	Poranee Banyatworakul
title	Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro
title_short	Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro
title_full	Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro
title_fullStr	Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro
title_full_unstemmed	Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro
title_sort	responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e7e8f6d8164e4868b5db20f03b5b2414
work_keys_str_mv	AT poraneebanyatworakul responsesofcanineperiodontalligamentcellstobubalinebloodderivedplateletrichfibrininvitro AT thanaphumosathanon responsesofcanineperiodontalligamentcellstobubalinebloodderivedplateletrichfibrininvitro AT sujinchumprasert responsesofcanineperiodontalligamentcellstobubalinebloodderivedplateletrichfibrininvitro AT prasitpavasant responsesofcanineperiodontalligamentcellstobubalinebloodderivedplateletrichfibrininvitro AT nopadonpirarat responsesofcanineperiodontalligamentcellstobubalinebloodderivedplateletrichfibrininvitro
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Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro

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