Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth

Abstract Indole-3-acetic acid (IAA) is the most common plant hormone of the auxin class and regulates various plant growth processes. The present study investigated IAA production by the basidiomycetous yeast Rhodosporidiobolus fluvialis DMKU-CP293 using the one-factor-at-a-time (OFAT) method and re...

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Autores principales:	Sakaoduoen Bunsangiam, Nutnaree Thongpae, Savitree Limtong, Nantana Srisuk
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e7ef4afa54bb42abacf22ed11f95afb8
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spelling	oai:doaj.org-article:e7ef4afa54bb42abacf22ed11f95afb82021-12-02T17:45:17ZLarge scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth10.1038/s41598-021-92305-w2045-2322https://doaj.org/article/e7ef4afa54bb42abacf22ed11f95afb82021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92305-whttps://doaj.org/toc/2045-2322Abstract Indole-3-acetic acid (IAA) is the most common plant hormone of the auxin class and regulates various plant growth processes. The present study investigated IAA production by the basidiomycetous yeast Rhodosporidiobolus fluvialis DMKU-CP293 using the one-factor-at-a-time (OFAT) method and response surface methodology (RSM). IAA production was optimized in shake-flask culture using a cost-effective medium containing 4.5% crude glycerol, 2% CSL and 0.55% feed-grade l-tryptophan. The optimized medium resulted in a 3.3-fold improvement in IAA production and a 3.6-fold reduction in cost compared with those obtained with a non-optimized medium. Production was then scaled up to a 15-L bioreactor and to a pilot-scale (100-L) bioreactor based on the constant impeller tip speed (Vtip) strategy. By doing so, IAA was successfully produced at a concentration of 3569.32 mg/L at the pilot scale. To the best of our knowledge, this is the first report of pilot-scale IAA production by microorganisms. In addition, we evaluated the effect of crude IAA on weed growth. The results showed that weed (Cyperus rotundus L.) growth could be inhibited by 50 mg/L of crude IAA. IAA therefore has the potential to be developed as a herbicidal bioproduct to replace the chemical herbicides that have been banned in various countries, including Thailand.Sakaoduoen BunsangiamNutnaree ThongpaeSavitree LimtongNantana SrisukNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sakaoduoen Bunsangiam Nutnaree Thongpae Savitree Limtong Nantana Srisuk Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth
description	Abstract Indole-3-acetic acid (IAA) is the most common plant hormone of the auxin class and regulates various plant growth processes. The present study investigated IAA production by the basidiomycetous yeast Rhodosporidiobolus fluvialis DMKU-CP293 using the one-factor-at-a-time (OFAT) method and response surface methodology (RSM). IAA production was optimized in shake-flask culture using a cost-effective medium containing 4.5% crude glycerol, 2% CSL and 0.55% feed-grade l-tryptophan. The optimized medium resulted in a 3.3-fold improvement in IAA production and a 3.6-fold reduction in cost compared with those obtained with a non-optimized medium. Production was then scaled up to a 15-L bioreactor and to a pilot-scale (100-L) bioreactor based on the constant impeller tip speed (Vtip) strategy. By doing so, IAA was successfully produced at a concentration of 3569.32 mg/L at the pilot scale. To the best of our knowledge, this is the first report of pilot-scale IAA production by microorganisms. In addition, we evaluated the effect of crude IAA on weed growth. The results showed that weed (Cyperus rotundus L.) growth could be inhibited by 50 mg/L of crude IAA. IAA therefore has the potential to be developed as a herbicidal bioproduct to replace the chemical herbicides that have been banned in various countries, including Thailand.
format	article
author	Sakaoduoen Bunsangiam Nutnaree Thongpae Savitree Limtong Nantana Srisuk
author_facet	Sakaoduoen Bunsangiam Nutnaree Thongpae Savitree Limtong Nantana Srisuk
author_sort	Sakaoduoen Bunsangiam
title	Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth
title_short	Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth
title_full	Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth
title_fullStr	Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth
title_full_unstemmed	Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth
title_sort	large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e7ef4afa54bb42abacf22ed11f95afb8
work_keys_str_mv	AT sakaoduoenbunsangiam largescaleproductionofindole3aceticacidandevaluationoftheinhibitoryeffectofindole3aceticacidonweedgrowth AT nutnareethongpae largescaleproductionofindole3aceticacidandevaluationoftheinhibitoryeffectofindole3aceticacidonweedgrowth AT savitreelimtong largescaleproductionofindole3aceticacidandevaluationoftheinhibitoryeffectofindole3aceticacidonweedgrowth AT nantanasrisuk largescaleproductionofindole3aceticacidandevaluationoftheinhibitoryeffectofindole3aceticacidonweedgrowth
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Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth

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