Verification and dissection of one quantitative trait locus for grain size and weight on chromosome 1 in rice

Abstract Grain size and weight are the key traits determining rice quality and yield and are mainly controlled by quantitative trait loci (QTL). In this study, one minor QTL that was previously mapped in the marker interval of JD1009-JD1019 using the Huanghuazhan/Jizi1560 (HHZ/JZ1560) recombinant in...

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Autores principales:	Yi-chen Cheng, Guan Li, Man Yin, Tosin Victor Adegoke, Yi-feng Wang, Xiao-hong Tong, Jian Zhang, Jie-zheng Ying
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e7f33f5882544bdaa38e0c4b04b2dfa7
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spelling	oai:doaj.org-article:e7f33f5882544bdaa38e0c4b04b2dfa72021-12-02T15:15:35ZVerification and dissection of one quantitative trait locus for grain size and weight on chromosome 1 in rice10.1038/s41598-021-97622-82045-2322https://doaj.org/article/e7f33f5882544bdaa38e0c4b04b2dfa72021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97622-8https://doaj.org/toc/2045-2322Abstract Grain size and weight are the key traits determining rice quality and yield and are mainly controlled by quantitative trait loci (QTL). In this study, one minor QTL that was previously mapped in the marker interval of JD1009-JD1019 using the Huanghuazhan/Jizi1560 (HHZ/JZ1560) recombinant inbred line (RIL) population, qTGW1-2, was validated to regulate grain size and weight across four rice-growing seasons using twenty-one near isogenic line (NIL)-F2 populations. The twenty-one populations were in two types of genetic background that were derived from the same parents HHZ and JZ1560. Twelve F9, F10 or F11 NIL-F2 populations with the sequential residual heterozygous regions covering JD1009-RM6840 were developed from one residual heterozygote (RH) in the HHZ/JZ1560 RIL population, and the remaining nine BC3F3, BC3F4 or BC3F5 NIL-F2 populations with the sequential residual heterozygous regions covering JD1009-RM6840 were constructed through consecutive backcrosses to the recurrent parent HHZ followed with marker assistant selection in each generation. Based on the QTL analysis of these genetic populations, qTGW1-2 was successfully confirmed to control grain length, width and weight and further dissected into two QTLs, qTGW1-2a and qTGW1-2b, which were respectively narrowed down to the marker intervals of JD1139-JD1127 (~ 978.2-kb) and JD1121-JD1102 (~ 54.8-kb). Furthermore, the two types of NIL-F2 populations were proved to be able to decrease the genetic background noise and increase the detection power of minor QTL. These results provided an important basis for further map-based cloning and molecular design breeding with the two QTLs in rice.Yi-chen ChengGuan LiMan YinTosin Victor AdegokeYi-feng WangXiao-hong TongJian ZhangJie-zheng YingNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi-chen Cheng Guan Li Man Yin Tosin Victor Adegoke Yi-feng Wang Xiao-hong Tong Jian Zhang Jie-zheng Ying Verification and dissection of one quantitative trait locus for grain size and weight on chromosome 1 in rice
description	Abstract Grain size and weight are the key traits determining rice quality and yield and are mainly controlled by quantitative trait loci (QTL). In this study, one minor QTL that was previously mapped in the marker interval of JD1009-JD1019 using the Huanghuazhan/Jizi1560 (HHZ/JZ1560) recombinant inbred line (RIL) population, qTGW1-2, was validated to regulate grain size and weight across four rice-growing seasons using twenty-one near isogenic line (NIL)-F2 populations. The twenty-one populations were in two types of genetic background that were derived from the same parents HHZ and JZ1560. Twelve F9, F10 or F11 NIL-F2 populations with the sequential residual heterozygous regions covering JD1009-RM6840 were developed from one residual heterozygote (RH) in the HHZ/JZ1560 RIL population, and the remaining nine BC3F3, BC3F4 or BC3F5 NIL-F2 populations with the sequential residual heterozygous regions covering JD1009-RM6840 were constructed through consecutive backcrosses to the recurrent parent HHZ followed with marker assistant selection in each generation. Based on the QTL analysis of these genetic populations, qTGW1-2 was successfully confirmed to control grain length, width and weight and further dissected into two QTLs, qTGW1-2a and qTGW1-2b, which were respectively narrowed down to the marker intervals of JD1139-JD1127 (~ 978.2-kb) and JD1121-JD1102 (~ 54.8-kb). Furthermore, the two types of NIL-F2 populations were proved to be able to decrease the genetic background noise and increase the detection power of minor QTL. These results provided an important basis for further map-based cloning and molecular design breeding with the two QTLs in rice.
format	article
author	Yi-chen Cheng Guan Li Man Yin Tosin Victor Adegoke Yi-feng Wang Xiao-hong Tong Jian Zhang Jie-zheng Ying
author_facet	Yi-chen Cheng Guan Li Man Yin Tosin Victor Adegoke Yi-feng Wang Xiao-hong Tong Jian Zhang Jie-zheng Ying
author_sort	Yi-chen Cheng
title	Verification and dissection of one quantitative trait locus for grain size and weight on chromosome 1 in rice
title_short	Verification and dissection of one quantitative trait locus for grain size and weight on chromosome 1 in rice
title_full	Verification and dissection of one quantitative trait locus for grain size and weight on chromosome 1 in rice
title_fullStr	Verification and dissection of one quantitative trait locus for grain size and weight on chromosome 1 in rice
title_full_unstemmed	Verification and dissection of one quantitative trait locus for grain size and weight on chromosome 1 in rice
title_sort	verification and dissection of one quantitative trait locus for grain size and weight on chromosome 1 in rice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e7f33f5882544bdaa38e0c4b04b2dfa7
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_version_	1718387543413620736

Verification and dissection of one quantitative trait locus for grain size and weight on chromosome 1 in rice

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