RIM1/2 in retinal ganglion cells are required for the refinement of ipsilateral axons and eye-specific segregation

Abstract Neural activity is crucial for the refinement of neuronal connections during development, but the contribution of synaptic release mechanisms is not known. In the mammalian retina, spontaneous neural activity controls the refinement of retinal projections to the dorsal lateral geniculate nu...

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Autores principales:	Ahlem Assali, Corentin Le Magueresse, Mohamed Bennis, Xavier Nicol, Patricia Gaspar, Alexandra Rebsam
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e7f35dec4915410eaad8ad339a4b94c1
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spelling	oai:doaj.org-article:e7f35dec4915410eaad8ad339a4b94c12021-12-02T11:52:41ZRIM1/2 in retinal ganglion cells are required for the refinement of ipsilateral axons and eye-specific segregation10.1038/s41598-017-03361-02045-2322https://doaj.org/article/e7f35dec4915410eaad8ad339a4b94c12017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03361-0https://doaj.org/toc/2045-2322Abstract Neural activity is crucial for the refinement of neuronal connections during development, but the contribution of synaptic release mechanisms is not known. In the mammalian retina, spontaneous neural activity controls the refinement of retinal projections to the dorsal lateral geniculate nucleus (dLGN) and the superior colliculus (SC) to form appropriate topographic and eye-specific maps. To evaluate the role of synaptic release, the rab-interacting molecules (RIMs), a family of active zone proteins that play a central role in calcium-triggered release, were conditionally ablated in a subset of retinal ganglion cells (RGCs). We found that this deletion is sufficient to reduce presynaptic release probability onto dLGN neurons. Furthermore, eye-specific segregation in the dLGN and topographic refinement of ipsilateral axons in the SC and the dLGN, are impaired in RIM1/2 conditional knock-out (Rim-cDKO) mice. These defects are similar to those found when retinal activity is globally disturbed. However, reduction in synaptic release had no effect on eye-specific lamination in the SC nor on the retinotopic refinement of contralateral axons in the SC. This study highlights a potential distinction between synaptic and non-synaptic roles of neuronal activity for different mapping rules operating in visual system development.Ahlem AssaliCorentin Le MagueresseMohamed BennisXavier NicolPatricia GasparAlexandra RebsamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ahlem Assali Corentin Le Magueresse Mohamed Bennis Xavier Nicol Patricia Gaspar Alexandra Rebsam RIM1/2 in retinal ganglion cells are required for the refinement of ipsilateral axons and eye-specific segregation
description	Abstract Neural activity is crucial for the refinement of neuronal connections during development, but the contribution of synaptic release mechanisms is not known. In the mammalian retina, spontaneous neural activity controls the refinement of retinal projections to the dorsal lateral geniculate nucleus (dLGN) and the superior colliculus (SC) to form appropriate topographic and eye-specific maps. To evaluate the role of synaptic release, the rab-interacting molecules (RIMs), a family of active zone proteins that play a central role in calcium-triggered release, were conditionally ablated in a subset of retinal ganglion cells (RGCs). We found that this deletion is sufficient to reduce presynaptic release probability onto dLGN neurons. Furthermore, eye-specific segregation in the dLGN and topographic refinement of ipsilateral axons in the SC and the dLGN, are impaired in RIM1/2 conditional knock-out (Rim-cDKO) mice. These defects are similar to those found when retinal activity is globally disturbed. However, reduction in synaptic release had no effect on eye-specific lamination in the SC nor on the retinotopic refinement of contralateral axons in the SC. This study highlights a potential distinction between synaptic and non-synaptic roles of neuronal activity for different mapping rules operating in visual system development.
format	article
author	Ahlem Assali Corentin Le Magueresse Mohamed Bennis Xavier Nicol Patricia Gaspar Alexandra Rebsam
author_facet	Ahlem Assali Corentin Le Magueresse Mohamed Bennis Xavier Nicol Patricia Gaspar Alexandra Rebsam
author_sort	Ahlem Assali
title	RIM1/2 in retinal ganglion cells are required for the refinement of ipsilateral axons and eye-specific segregation
title_short	RIM1/2 in retinal ganglion cells are required for the refinement of ipsilateral axons and eye-specific segregation
title_full	RIM1/2 in retinal ganglion cells are required for the refinement of ipsilateral axons and eye-specific segregation
title_fullStr	RIM1/2 in retinal ganglion cells are required for the refinement of ipsilateral axons and eye-specific segregation
title_full_unstemmed	RIM1/2 in retinal ganglion cells are required for the refinement of ipsilateral axons and eye-specific segregation
title_sort	rim1/2 in retinal ganglion cells are required for the refinement of ipsilateral axons and eye-specific segregation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e7f35dec4915410eaad8ad339a4b94c1
work_keys_str_mv	AT ahlemassali rim12inretinalganglioncellsarerequiredfortherefinementofipsilateralaxonsandeyespecificsegregation AT corentinlemagueresse rim12inretinalganglioncellsarerequiredfortherefinementofipsilateralaxonsandeyespecificsegregation AT mohamedbennis rim12inretinalganglioncellsarerequiredfortherefinementofipsilateralaxonsandeyespecificsegregation AT xaviernicol rim12inretinalganglioncellsarerequiredfortherefinementofipsilateralaxonsandeyespecificsegregation AT patriciagaspar rim12inretinalganglioncellsarerequiredfortherefinementofipsilateralaxonsandeyespecificsegregation AT alexandrarebsam rim12inretinalganglioncellsarerequiredfortherefinementofipsilateralaxonsandeyespecificsegregation
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RIM1/2 in retinal ganglion cells are required for the refinement of ipsilateral axons and eye-specific segregation

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