Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss

Abstract Nowadays, with the development and advancement of next-generation sequencing technologies, a new path has been provided for transcriptomic studies. In this study, the transcriptome of Dracocephalum kotschyi Boiss., as an endemic and endangered plant which is contained a large amount of valu...

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Autores principales:	Abdonaser Poursalavati, Sajad Rashidi-Monfared, Amin Ebrahimi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e7f4ce12677344d58896e53f0755e0a3
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spelling	oai:doaj.org-article:e7f4ce12677344d58896e53f0755e0a32021-12-02T17:18:20ZToward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss10.1038/s41598-021-99066-62045-2322https://doaj.org/article/e7f4ce12677344d58896e53f0755e0a32021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99066-6https://doaj.org/toc/2045-2322Abstract Nowadays, with the development and advancement of next-generation sequencing technologies, a new path has been provided for transcriptomic studies. In this study, the transcriptome of Dracocephalum kotschyi Boiss., as an endemic and endangered plant which is contained a large amount of valuable secondary metabolites with antioxidant and anticancer properties, was sequenced. Then functional annotation and gene ontology analysis for 165,597 assembled transcripts were performed, most were associated with the metabolic pathways. This might be because there are various active biochemical pathways in this plant. Furthermore, after comprehensive transcript annotation, the putative genes involved in the main metabolic pathways of D. kotschyi were identified. Then, the biosynthetic pathway of its valuable methoxylated flavones was proposed. Finally, the accumulations of important methoxylated-flavone metabolites in three different tissues were quantified by HPLC. The relative expression of the genes involved in the proposed pathway was investigated by qRT-PCR, which indicated high expression levels in the bud tissue. The present results may lead to the design strategies to preserve the genetic diversity of endangered D. kotschyi plants and apply the new methods for engineering its valuable methoxylated-flavones pathway.Abdonaser PoursalavatiSajad Rashidi-MonfaredAmin EbrahimiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abdonaser Poursalavati Sajad Rashidi-Monfared Amin Ebrahimi Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss
description	Abstract Nowadays, with the development and advancement of next-generation sequencing technologies, a new path has been provided for transcriptomic studies. In this study, the transcriptome of Dracocephalum kotschyi Boiss., as an endemic and endangered plant which is contained a large amount of valuable secondary metabolites with antioxidant and anticancer properties, was sequenced. Then functional annotation and gene ontology analysis for 165,597 assembled transcripts were performed, most were associated with the metabolic pathways. This might be because there are various active biochemical pathways in this plant. Furthermore, after comprehensive transcript annotation, the putative genes involved in the main metabolic pathways of D. kotschyi were identified. Then, the biosynthetic pathway of its valuable methoxylated flavones was proposed. Finally, the accumulations of important methoxylated-flavone metabolites in three different tissues were quantified by HPLC. The relative expression of the genes involved in the proposed pathway was investigated by qRT-PCR, which indicated high expression levels in the bud tissue. The present results may lead to the design strategies to preserve the genetic diversity of endangered D. kotschyi plants and apply the new methods for engineering its valuable methoxylated-flavones pathway.
format	article
author	Abdonaser Poursalavati Sajad Rashidi-Monfared Amin Ebrahimi
author_facet	Abdonaser Poursalavati Sajad Rashidi-Monfared Amin Ebrahimi
author_sort	Abdonaser Poursalavati
title	Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss
title_short	Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss
title_full	Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss
title_fullStr	Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss
title_full_unstemmed	Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss
title_sort	toward understanding of the methoxylated flavonoid biosynthesis pathway in dracocephalum kotschyi boiss
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e7f4ce12677344d58896e53f0755e0a3
work_keys_str_mv	AT abdonaserpoursalavati towardunderstandingofthemethoxylatedflavonoidbiosynthesispathwayindracocephalumkotschyiboiss AT sajadrashidimonfared towardunderstandingofthemethoxylatedflavonoidbiosynthesispathwayindracocephalumkotschyiboiss AT aminebrahimi towardunderstandingofthemethoxylatedflavonoidbiosynthesispathwayindracocephalumkotschyiboiss
_version_	1718381117305782272

Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss

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