In vitro and in vivo evaluation of a standardized Curcuma longa Linn formulation in cervical cancer

Background: The anti-cancer activity of phytomolecules present in turmeric or haridra (Curcuma longa Linn) extracts against cancer has been described in various ‘in vitro and in vivo’ studies. Objective: In the present study, in vitro and in vivo anti-cancer and chemo-preventive activity of a new st...

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Autores principales:	P.H. Paradkar, A.S. Juvekar, M.S. Barkume, A.J. Amonkar, J.V. Joshi, G. Soman, A.D.B. Vaidya
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cervical cancer Chemoprevention Anticancer Medicinal plants Supercritical turmeric oil extract Prevention of cervical cancer Miscellaneous systems and treatments RZ409.7-999
Acceso en línea:	https://doaj.org/article/e7fe71b5d66d427697e2645456376bdd
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spelling	oai:doaj.org-article:e7fe71b5d66d427697e2645456376bdd2021-12-02T04:59:19ZIn vitro and in vivo evaluation of a standardized Curcuma longa Linn formulation in cervical cancer0975-947610.1016/j.jaim.2021.06.002https://doaj.org/article/e7fe71b5d66d427697e2645456376bdd2021-10-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0975947621001108https://doaj.org/toc/0975-9476Background: The anti-cancer activity of phytomolecules present in turmeric or haridra (Curcuma longa Linn) extracts against cancer has been described in various ‘in vitro and in vivo’ studies. Objective: In the present study, in vitro and in vivo anti-cancer and chemo-preventive activity of a new standardized Supercritical Turmeric Oil Extract (SCTOE) NBFR-03 was evaluated in cervical cancer models. Methods and materials: In vitro cytotoxicity of this formulation was assessed at 10, 20, 40, and 80 μg/ml concentrations, in three cervical cancer cell lines (HeLa, SiHa, ME180) using Sulforhodamine B assay. The in vivo anti-cancer activity was evaluated in two groups of female nude mice; the first one was with tumor xenograft implants and at the same time treatment was started with 96 μl/kg/day p.o. and 192 μl/kg/day p.o. NBFR-03 for three months. The second group was kept as chemoprevention group where mice were pre-treated with the formulation (96 μl/kg/day p.o.) for two weeks and injected with cancer cell suspension with continued treatment for three months. Results: No cytotoxicity was seen in any cell line with the extract when compared to positive control (Adriamycin 10 μg/ml). In mice the first treatment group with tumor xenograft implants did not show any significant anti-tumor activity but showed a trend where higher dose group had smaller tumor volumes as compared to lower dose group and controls (p = 0.37 and p = 0.34 respectively). The chemopreventive group with pre-treated mice also showed smaller tumor size as compared to controls (p = 0.163). Conclusion: NBFR-03 turmeric oil extract showed a promising trend in mice pre-treated with NBFR-03. There is a scope for further studying the potential of this extract as complementary therapy and as a chemopreventive.P.H. ParadkarA.S. JuvekarM.S. BarkumeA.J. AmonkarJ.V. JoshiG. SomanA.D.B. VaidyaElsevierarticleCervical cancerChemopreventionAnticancerMedicinal plantsSupercritical turmeric oil extractPrevention of cervical cancerMiscellaneous systems and treatmentsRZ409.7-999ENJournal of Ayurveda and Integrative Medicine, Vol 12, Iss 4, Pp 616-622 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cervical cancer Chemoprevention Anticancer Medicinal plants Supercritical turmeric oil extract Prevention of cervical cancer Miscellaneous systems and treatments RZ409.7-999
spellingShingle	Cervical cancer Chemoprevention Anticancer Medicinal plants Supercritical turmeric oil extract Prevention of cervical cancer Miscellaneous systems and treatments RZ409.7-999 P.H. Paradkar A.S. Juvekar M.S. Barkume A.J. Amonkar J.V. Joshi G. Soman A.D.B. Vaidya In vitro and in vivo evaluation of a standardized Curcuma longa Linn formulation in cervical cancer
description	Background: The anti-cancer activity of phytomolecules present in turmeric or haridra (Curcuma longa Linn) extracts against cancer has been described in various ‘in vitro and in vivo’ studies. Objective: In the present study, in vitro and in vivo anti-cancer and chemo-preventive activity of a new standardized Supercritical Turmeric Oil Extract (SCTOE) NBFR-03 was evaluated in cervical cancer models. Methods and materials: In vitro cytotoxicity of this formulation was assessed at 10, 20, 40, and 80 μg/ml concentrations, in three cervical cancer cell lines (HeLa, SiHa, ME180) using Sulforhodamine B assay. The in vivo anti-cancer activity was evaluated in two groups of female nude mice; the first one was with tumor xenograft implants and at the same time treatment was started with 96 μl/kg/day p.o. and 192 μl/kg/day p.o. NBFR-03 for three months. The second group was kept as chemoprevention group where mice were pre-treated with the formulation (96 μl/kg/day p.o.) for two weeks and injected with cancer cell suspension with continued treatment for three months. Results: No cytotoxicity was seen in any cell line with the extract when compared to positive control (Adriamycin 10 μg/ml). In mice the first treatment group with tumor xenograft implants did not show any significant anti-tumor activity but showed a trend where higher dose group had smaller tumor volumes as compared to lower dose group and controls (p = 0.37 and p = 0.34 respectively). The chemopreventive group with pre-treated mice also showed smaller tumor size as compared to controls (p = 0.163). Conclusion: NBFR-03 turmeric oil extract showed a promising trend in mice pre-treated with NBFR-03. There is a scope for further studying the potential of this extract as complementary therapy and as a chemopreventive.
format	article
author	P.H. Paradkar A.S. Juvekar M.S. Barkume A.J. Amonkar J.V. Joshi G. Soman A.D.B. Vaidya
author_facet	P.H. Paradkar A.S. Juvekar M.S. Barkume A.J. Amonkar J.V. Joshi G. Soman A.D.B. Vaidya
author_sort	P.H. Paradkar
title	In vitro and in vivo evaluation of a standardized Curcuma longa Linn formulation in cervical cancer
title_short	In vitro and in vivo evaluation of a standardized Curcuma longa Linn formulation in cervical cancer
title_full	In vitro and in vivo evaluation of a standardized Curcuma longa Linn formulation in cervical cancer
title_fullStr	In vitro and in vivo evaluation of a standardized Curcuma longa Linn formulation in cervical cancer
title_full_unstemmed	In vitro and in vivo evaluation of a standardized Curcuma longa Linn formulation in cervical cancer
title_sort	in vitro and in vivo evaluation of a standardized curcuma longa linn formulation in cervical cancer
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e7fe71b5d66d427697e2645456376bdd
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In vitro and in vivo evaluation of a standardized Curcuma longa Linn formulation in cervical cancer

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