Effect of Quercetin on Injury to Indomethacin-Treated Human Embryonic Kidney 293 Cells

Nonsteroidal anti-inflammatory drugs (NSAIDs) are used to treat inflammation and pain and even to prevent the progression of cardiovascular disease. They have become widely used because of their effectiveness, especially among athletes performing high-intensity training. Indomethacin is used for pai...

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Autores principales:	Chun Chen, Jai-Sing Yang, Chi-Cheng Lu, Yu-Tse Wu, Fu-An Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	apoptosis antioxidant human embryonic kidney 293 (HEK293) cells indomethacin quercetin mitochondrial membrane potential (ΔΨm) Science Q
Acceso en línea:	https://doaj.org/article/e803434e85a44652ac5356929025dda6
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spelling	oai:doaj.org-article:e803434e85a44652ac5356929025dda62021-11-25T18:10:33ZEffect of Quercetin on Injury to Indomethacin-Treated Human Embryonic Kidney 293 Cells10.3390/life111111342075-1729https://doaj.org/article/e803434e85a44652ac5356929025dda62021-10-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1134https://doaj.org/toc/2075-1729Nonsteroidal anti-inflammatory drugs (NSAIDs) are used to treat inflammation and pain and even to prevent the progression of cardiovascular disease. They have become widely used because of their effectiveness, especially among athletes performing high-intensity training. Indomethacin is used for pain management in sports medicine and is highly effective and versatile. However, several clinical studies have reported that indomethacin induces acute renal damage. In the present study, we determined that indomethacin reduced human embryonic kidney 293 (HEK293) cell viability in a concentration-dependent manner by triggering apoptosis. In addition, we demonstrated the effect of quercetin on indomethacin-treated HEK293 cells by inactivating the caspase-3 and caspase-9 signals. Furthermore, quercetin reduced ROS production and increased mitochondrial membrane potential (ΔΨm) in indomethacin-treated HEK293 cells. Our results indicate that quercetin can interrupt the activated caspase and mitochondrial pathway induced by indomethacin in HEK293 cells and affect apoptotic mRNA expression. Quercetin can protect against indomethacin-induced HEK293 cell apoptosis by regulating abnormal ΔΨm and apoptotic mRNA expression.Chun ChenJai-Sing YangChi-Cheng LuYu-Tse WuFu-An ChenMDPI AGarticleapoptosisantioxidanthuman embryonic kidney 293 (HEK293) cellsindomethacinquercetinmitochondrial membrane potential (ΔΨm)ScienceQENLife, Vol 11, Iss 1134, p 1134 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	apoptosis antioxidant human embryonic kidney 293 (HEK293) cells indomethacin quercetin mitochondrial membrane potential (ΔΨm) Science Q
spellingShingle	apoptosis antioxidant human embryonic kidney 293 (HEK293) cells indomethacin quercetin mitochondrial membrane potential (ΔΨm) Science Q Chun Chen Jai-Sing Yang Chi-Cheng Lu Yu-Tse Wu Fu-An Chen Effect of Quercetin on Injury to Indomethacin-Treated Human Embryonic Kidney 293 Cells
description	Nonsteroidal anti-inflammatory drugs (NSAIDs) are used to treat inflammation and pain and even to prevent the progression of cardiovascular disease. They have become widely used because of their effectiveness, especially among athletes performing high-intensity training. Indomethacin is used for pain management in sports medicine and is highly effective and versatile. However, several clinical studies have reported that indomethacin induces acute renal damage. In the present study, we determined that indomethacin reduced human embryonic kidney 293 (HEK293) cell viability in a concentration-dependent manner by triggering apoptosis. In addition, we demonstrated the effect of quercetin on indomethacin-treated HEK293 cells by inactivating the caspase-3 and caspase-9 signals. Furthermore, quercetin reduced ROS production and increased mitochondrial membrane potential (ΔΨm) in indomethacin-treated HEK293 cells. Our results indicate that quercetin can interrupt the activated caspase and mitochondrial pathway induced by indomethacin in HEK293 cells and affect apoptotic mRNA expression. Quercetin can protect against indomethacin-induced HEK293 cell apoptosis by regulating abnormal ΔΨm and apoptotic mRNA expression.
format	article
author	Chun Chen Jai-Sing Yang Chi-Cheng Lu Yu-Tse Wu Fu-An Chen
author_facet	Chun Chen Jai-Sing Yang Chi-Cheng Lu Yu-Tse Wu Fu-An Chen
author_sort	Chun Chen
title	Effect of Quercetin on Injury to Indomethacin-Treated Human Embryonic Kidney 293 Cells
title_short	Effect of Quercetin on Injury to Indomethacin-Treated Human Embryonic Kidney 293 Cells
title_full	Effect of Quercetin on Injury to Indomethacin-Treated Human Embryonic Kidney 293 Cells
title_fullStr	Effect of Quercetin on Injury to Indomethacin-Treated Human Embryonic Kidney 293 Cells
title_full_unstemmed	Effect of Quercetin on Injury to Indomethacin-Treated Human Embryonic Kidney 293 Cells
title_sort	effect of quercetin on injury to indomethacin-treated human embryonic kidney 293 cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e803434e85a44652ac5356929025dda6
work_keys_str_mv	AT chunchen effectofquercetinoninjurytoindomethacintreatedhumanembryonickidney293cells AT jaisingyang effectofquercetinoninjurytoindomethacintreatedhumanembryonickidney293cells AT chichenglu effectofquercetinoninjurytoindomethacintreatedhumanembryonickidney293cells AT yutsewu effectofquercetinoninjurytoindomethacintreatedhumanembryonickidney293cells AT fuanchen effectofquercetinoninjurytoindomethacintreatedhumanembryonickidney293cells
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Effect of Quercetin on Injury to Indomethacin-Treated Human Embryonic Kidney 293 Cells

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