Voltammetric Immunosensor to Track a Major Peanut Allergen (Ara h 1) in Food Products Employing Quantum Dot Labels

Tracking unreported allergens in commercial foods can avoid acute allergic reactions. A 2-step electrochemical immunosensor was developed for the analysis of the peanut allergen Ara h 1 in a 1-h assay (<15 min hands-on time). Bare screen-printed carbon electrodes (SPCE) were used as transducers a...

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Autores principales:	Maria Freitas, Henri P. A. Nouws, Cristina Delerue-Matos
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Ara h 1 biosensor electrochemical immunosensor food allergy nanolabel peanut allergy Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/e804088ab8744b12a736202ac88592d2
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id	oai:doaj.org-article:e804088ab8744b12a736202ac88592d2
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spelling	oai:doaj.org-article:e804088ab8744b12a736202ac88592d22021-11-25T16:55:07ZVoltammetric Immunosensor to Track a Major Peanut Allergen (Ara h 1) in Food Products Employing Quantum Dot Labels10.3390/bios111104262079-6374https://doaj.org/article/e804088ab8744b12a736202ac88592d22021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/426https://doaj.org/toc/2079-6374Tracking unreported allergens in commercial foods can avoid acute allergic reactions. A 2-step electrochemical immunosensor was developed for the analysis of the peanut allergen Ara h 1 in a 1-h assay (<15 min hands-on time). Bare screen-printed carbon electrodes (SPCE) were used as transducers and monoclonal capture and detection antibodies were applied in a sandwich-type immunoassay. The short assay time was achieved by previously combining the target analyte and the detection antibody. Core/shell CdSe@ZnS Quantum Dots were used as electroactive label for the detection of the immunological interaction by differential pulse anodic stripping voltammetry. A linear range between 25 and 1000 ng·mL<sup>−1</sup> (LOD = 3.5 ng·mL<sup>−1</sup>), an adequate precision of the method (V<sub>x0</sub> ≈ 6%), and a sensitivity of 23.0 nA·mL·ng<sup>−1</sup>·cm<sup>−2</sup> were achieved. The immunosensor was able to detect Ara h 1 in a spiked allergen-free product down to 0.05% (m/m) of peanut. Commercial organic farming cookies and cereal and protein bars were tested to track and quantify Ara h 1. The results were validated by comparison with an ELISA kit.Maria FreitasHenri P. A. NouwsCristina Delerue-MatosMDPI AGarticleAra h 1biosensorelectrochemical immunosensorfood allergynanolabelpeanut allergyBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 426, p 426 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ara h 1 biosensor electrochemical immunosensor food allergy nanolabel peanut allergy Biotechnology TP248.13-248.65
spellingShingle	Ara h 1 biosensor electrochemical immunosensor food allergy nanolabel peanut allergy Biotechnology TP248.13-248.65 Maria Freitas Henri P. A. Nouws Cristina Delerue-Matos Voltammetric Immunosensor to Track a Major Peanut Allergen (Ara h 1) in Food Products Employing Quantum Dot Labels
description	Tracking unreported allergens in commercial foods can avoid acute allergic reactions. A 2-step electrochemical immunosensor was developed for the analysis of the peanut allergen Ara h 1 in a 1-h assay (<15 min hands-on time). Bare screen-printed carbon electrodes (SPCE) were used as transducers and monoclonal capture and detection antibodies were applied in a sandwich-type immunoassay. The short assay time was achieved by previously combining the target analyte and the detection antibody. Core/shell CdSe@ZnS Quantum Dots were used as electroactive label for the detection of the immunological interaction by differential pulse anodic stripping voltammetry. A linear range between 25 and 1000 ng·mL<sup>−1</sup> (LOD = 3.5 ng·mL<sup>−1</sup>), an adequate precision of the method (V<sub>x0</sub> ≈ 6%), and a sensitivity of 23.0 nA·mL·ng<sup>−1</sup>·cm<sup>−2</sup> were achieved. The immunosensor was able to detect Ara h 1 in a spiked allergen-free product down to 0.05% (m/m) of peanut. Commercial organic farming cookies and cereal and protein bars were tested to track and quantify Ara h 1. The results were validated by comparison with an ELISA kit.
format	article
author	Maria Freitas Henri P. A. Nouws Cristina Delerue-Matos
author_facet	Maria Freitas Henri P. A. Nouws Cristina Delerue-Matos
author_sort	Maria Freitas
title	Voltammetric Immunosensor to Track a Major Peanut Allergen (Ara h 1) in Food Products Employing Quantum Dot Labels
title_short	Voltammetric Immunosensor to Track a Major Peanut Allergen (Ara h 1) in Food Products Employing Quantum Dot Labels
title_full	Voltammetric Immunosensor to Track a Major Peanut Allergen (Ara h 1) in Food Products Employing Quantum Dot Labels
title_fullStr	Voltammetric Immunosensor to Track a Major Peanut Allergen (Ara h 1) in Food Products Employing Quantum Dot Labels
title_full_unstemmed	Voltammetric Immunosensor to Track a Major Peanut Allergen (Ara h 1) in Food Products Employing Quantum Dot Labels
title_sort	voltammetric immunosensor to track a major peanut allergen (ara h 1) in food products employing quantum dot labels
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e804088ab8744b12a736202ac88592d2
work_keys_str_mv	AT mariafreitas voltammetricimmunosensortotrackamajorpeanutallergenarah1infoodproductsemployingquantumdotlabels AT henripanouws voltammetricimmunosensortotrackamajorpeanutallergenarah1infoodproductsemployingquantumdotlabels AT cristinadeleruematos voltammetricimmunosensortotrackamajorpeanutallergenarah1infoodproductsemployingquantumdotlabels
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Voltammetric Immunosensor to Track a Major Peanut Allergen (Ara h 1) in Food Products Employing Quantum Dot Labels

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