Three-dimensional morphology of bacterial community developed on the index-matched materials

Abstract Herein, we demonstrate that the use of index-matching materials (IMMs) allows direct visualization of microbial cells maintained at a solid–liquid interface through confocal reflection microscopy (CRM). The refractive index mismatch induces a background reflection at the solid–liquid interf...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chigusa Okano, Kyosuke Takabe, Tomohiro Hirayama, Nobuhiko Nomura, Yutaka Yawata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e804f22cf6c148d6b94209d200fb369c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e804f22cf6c148d6b94209d200fb369c
record_format	dspace
spelling	oai:doaj.org-article:e804f22cf6c148d6b94209d200fb369c2021-12-02T17:17:40ZThree-dimensional morphology of bacterial community developed on the index-matched materials10.1038/s41598-021-98943-42045-2322https://doaj.org/article/e804f22cf6c148d6b94209d200fb369c2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98943-4https://doaj.org/toc/2045-2322Abstract Herein, we demonstrate that the use of index-matching materials (IMMs) allows direct visualization of microbial cells maintained at a solid–liquid interface through confocal reflection microscopy (CRM). The refractive index mismatch induces a background reflection at the solid–liquid interface that dwarfs the reflection signals from the cells and results in low-contrast images. We found that the IMMs sufficiently suppressed the background reflection at the solid–liquid interface, facilitating the imaging of microbes at the solid surface using CRM. The use of IMMs allowed quantitative analysis of the morphology of the mesh-like structure of Pseudomonas aeruginosa biofilms formed under denitrifying conditions, which led us to propose a novel structural model of the highly porous biofilm structure. These results indicate that the use of CRM coupled with an IMM offers a unique and promising tool for probing the dynamics of biofilm formation, along with visualization of environmental organisms and newly isolated bacteria, for which transformation methods are difficult to establish.Chigusa OkanoKyosuke TakabeTomohiro HirayamaNobuhiko NomuraYutaka YawataNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chigusa Okano Kyosuke Takabe Tomohiro Hirayama Nobuhiko Nomura Yutaka Yawata Three-dimensional morphology of bacterial community developed on the index-matched materials
description	Abstract Herein, we demonstrate that the use of index-matching materials (IMMs) allows direct visualization of microbial cells maintained at a solid–liquid interface through confocal reflection microscopy (CRM). The refractive index mismatch induces a background reflection at the solid–liquid interface that dwarfs the reflection signals from the cells and results in low-contrast images. We found that the IMMs sufficiently suppressed the background reflection at the solid–liquid interface, facilitating the imaging of microbes at the solid surface using CRM. The use of IMMs allowed quantitative analysis of the morphology of the mesh-like structure of Pseudomonas aeruginosa biofilms formed under denitrifying conditions, which led us to propose a novel structural model of the highly porous biofilm structure. These results indicate that the use of CRM coupled with an IMM offers a unique and promising tool for probing the dynamics of biofilm formation, along with visualization of environmental organisms and newly isolated bacteria, for which transformation methods are difficult to establish.
format	article
author	Chigusa Okano Kyosuke Takabe Tomohiro Hirayama Nobuhiko Nomura Yutaka Yawata
author_facet	Chigusa Okano Kyosuke Takabe Tomohiro Hirayama Nobuhiko Nomura Yutaka Yawata
author_sort	Chigusa Okano
title	Three-dimensional morphology of bacterial community developed on the index-matched materials
title_short	Three-dimensional morphology of bacterial community developed on the index-matched materials
title_full	Three-dimensional morphology of bacterial community developed on the index-matched materials
title_fullStr	Three-dimensional morphology of bacterial community developed on the index-matched materials
title_full_unstemmed	Three-dimensional morphology of bacterial community developed on the index-matched materials
title_sort	three-dimensional morphology of bacterial community developed on the index-matched materials
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e804f22cf6c148d6b94209d200fb369c
work_keys_str_mv	AT chigusaokano threedimensionalmorphologyofbacterialcommunitydevelopedontheindexmatchedmaterials AT kyosuketakabe threedimensionalmorphologyofbacterialcommunitydevelopedontheindexmatchedmaterials AT tomohirohirayama threedimensionalmorphologyofbacterialcommunitydevelopedontheindexmatchedmaterials AT nobuhikonomura threedimensionalmorphologyofbacterialcommunitydevelopedontheindexmatchedmaterials AT yutakayawata threedimensionalmorphologyofbacterialcommunitydevelopedontheindexmatchedmaterials
_version_	1718381175283646464

Three-dimensional morphology of bacterial community developed on the index-matched materials

Ejemplares similares