Pannexin 3 regulates skin development via Epiprofin

Abstract Pannexin 3 (Panx3), a member of the gap junction pannexin family is required for the development of hard tissues including bone, cartilage and teeth. However, the role of Panx3 in skin development remains unclear. Here, we demonstrate that Panx3 regulates skin development by modulating the...

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Autores principales:	Peipei Zhang, Masaki Ishikawa, Andrew Doyle, Takashi Nakamura, Bing He, Yoshihiko Yamada
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e8058379e5ad4a8197bd48d4c003ae68
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spelling	oai:doaj.org-article:e8058379e5ad4a8197bd48d4c003ae682021-12-02T11:50:40ZPannexin 3 regulates skin development via Epiprofin10.1038/s41598-021-81074-12045-2322https://doaj.org/article/e8058379e5ad4a8197bd48d4c003ae682021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81074-1https://doaj.org/toc/2045-2322Abstract Pannexin 3 (Panx3), a member of the gap junction pannexin family is required for the development of hard tissues including bone, cartilage and teeth. However, the role of Panx3 in skin development remains unclear. Here, we demonstrate that Panx3 regulates skin development by modulating the transcription factor, Epiprofin (Epfn). Panx3−/− mice have impaired skin development and delayed hair follicle regeneration. Loss of Panx3 in knockout mice and suppression by shRNA both elicited a reduction of Epfn expression in the epidermis. In cell culture, Panx3 overexpression promoted HaCaT cell differentiation, cell cycle exit and enhanced Epfn expression. Epfn−/− mice and inhibition of Epfn by siRNA showed no obvious differences of Panx3 expression. Furthermore, Panx3 promotes Akt/NFAT signaling pathway in keratinocyte differentiation by both Panx3 ATP releasing channel and ER Ca2+ channel functions. Our results reveal that Panx3 has a key role factor for the skin development by regulating Epfn.Peipei ZhangMasaki IshikawaAndrew DoyleTakashi NakamuraBing HeYoshihiko YamadaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peipei Zhang Masaki Ishikawa Andrew Doyle Takashi Nakamura Bing He Yoshihiko Yamada Pannexin 3 regulates skin development via Epiprofin
description	Abstract Pannexin 3 (Panx3), a member of the gap junction pannexin family is required for the development of hard tissues including bone, cartilage and teeth. However, the role of Panx3 in skin development remains unclear. Here, we demonstrate that Panx3 regulates skin development by modulating the transcription factor, Epiprofin (Epfn). Panx3−/− mice have impaired skin development and delayed hair follicle regeneration. Loss of Panx3 in knockout mice and suppression by shRNA both elicited a reduction of Epfn expression in the epidermis. In cell culture, Panx3 overexpression promoted HaCaT cell differentiation, cell cycle exit and enhanced Epfn expression. Epfn−/− mice and inhibition of Epfn by siRNA showed no obvious differences of Panx3 expression. Furthermore, Panx3 promotes Akt/NFAT signaling pathway in keratinocyte differentiation by both Panx3 ATP releasing channel and ER Ca2+ channel functions. Our results reveal that Panx3 has a key role factor for the skin development by regulating Epfn.
format	article
author	Peipei Zhang Masaki Ishikawa Andrew Doyle Takashi Nakamura Bing He Yoshihiko Yamada
author_facet	Peipei Zhang Masaki Ishikawa Andrew Doyle Takashi Nakamura Bing He Yoshihiko Yamada
author_sort	Peipei Zhang
title	Pannexin 3 regulates skin development via Epiprofin
title_short	Pannexin 3 regulates skin development via Epiprofin
title_full	Pannexin 3 regulates skin development via Epiprofin
title_fullStr	Pannexin 3 regulates skin development via Epiprofin
title_full_unstemmed	Pannexin 3 regulates skin development via Epiprofin
title_sort	pannexin 3 regulates skin development via epiprofin
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e8058379e5ad4a8197bd48d4c003ae68
work_keys_str_mv	AT peipeizhang pannexin3regulatesskindevelopmentviaepiprofin AT masakiishikawa pannexin3regulatesskindevelopmentviaepiprofin AT andrewdoyle pannexin3regulatesskindevelopmentviaepiprofin AT takashinakamura pannexin3regulatesskindevelopmentviaepiprofin AT binghe pannexin3regulatesskindevelopmentviaepiprofin AT yoshihikoyamada pannexin3regulatesskindevelopmentviaepiprofin
_version_	1718395197131325440

Pannexin 3 regulates skin development via Epiprofin

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