Unveiling Monoterpene Biosynthesis in <i>Taiwania cryptomerioides</i> via Functional Characterization
<i>Taiwania cryptomerioides</i> is a monotypic species, and its terpenoid-rich property has been reported in recent years. To uncover monoterpene biosynthesis in <i>T. cryptomerioides</i>, this study used transcriptome mining to identify candidates with tentative monoterpene...
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/e80d5fd2cd184926951939b794ab708c |
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| Sumario: | <i>Taiwania cryptomerioides</i> is a monotypic species, and its terpenoid-rich property has been reported in recent years. To uncover monoterpene biosynthesis in <i>T. cryptomerioides</i>, this study used transcriptome mining to identify candidates with tentative monoterpene synthase activity. Along with the phylogenetic analysis and in vitro assay, two geraniol synthases (<i>Tc</i>TPS13 and <i>Tc</i>TPS14), a linalool synthase (<i>Tc</i>TPS15), and a β-pinene synthase (<i>Tc</i>TPS16), were functionally characterized. Via the comparison of catalytic residues, the Cys/Ser at region 1 might be crucial in determining the formation of α-pinene or β-pinene. In addition, the Cupressaceae monoterpene synthases were phylogenetically clustered together; they are unique and different from those of published conifer species. In summary, this study aimed to uncover the ambiguous monoterpenoid network in <i>T. cryptomerioide</i>, which would expand the landscape of monoterpene biosynthesis in Cupressaceae species. |
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