Unveiling Monoterpene Biosynthesis in Taiwania cryptomerioides via Functional Characterization

Taiwania cryptomerioides is a monotypic species, and its terpenoid-rich property has been reported in recent years. To uncover monoterpene biosynthesis in T. cryptomerioides, this study used transcriptome mining to identify candidates with tentative monoterpene...

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Autores principales:	Li-Ting Ma, Pi-Ling Liu, Yang-Tui Cheng, Tz-Fan Shiu, Fang-Hua Chu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Taiwania cryptomerioides</i> monoterpene synthases gas chromatography-mass spectrometry (GC-MS) Botany QK1-989
Acceso en línea:	https://doaj.org/article/e80d5fd2cd184926951939b794ab708c
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spelling	oai:doaj.org-article:e80d5fd2cd184926951939b794ab708c2021-11-25T18:46:20ZUnveiling Monoterpene Biosynthesis in <i>Taiwania cryptomerioides</i> via Functional Characterization10.3390/plants101124042223-7747https://doaj.org/article/e80d5fd2cd184926951939b794ab708c2021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2404https://doaj.org/toc/2223-7747<i>Taiwania cryptomerioides</i> is a monotypic species, and its terpenoid-rich property has been reported in recent years. To uncover monoterpene biosynthesis in <i>T. cryptomerioides</i>, this study used transcriptome mining to identify candidates with tentative monoterpene synthase activity. Along with the phylogenetic analysis and in vitro assay, two geraniol synthases (<i>Tc</i>TPS13 and <i>Tc</i>TPS14), a linalool synthase (<i>Tc</i>TPS15), and a β-pinene synthase (<i>Tc</i>TPS16), were functionally characterized. Via the comparison of catalytic residues, the Cys/Ser at region 1 might be crucial in determining the formation of α-pinene or β-pinene. In addition, the Cupressaceae monoterpene synthases were phylogenetically clustered together; they are unique and different from those of published conifer species. In summary, this study aimed to uncover the ambiguous monoterpenoid network in <i>T. cryptomerioide</i>, which would expand the landscape of monoterpene biosynthesis in Cupressaceae species.Li-Ting MaPi-Ling LiuYang-Tui ChengTz-Fan ShiuFang-Hua ChuMDPI AGarticle<i>Taiwania cryptomerioides</i>monoterpene synthasesgas chromatography-mass spectrometry (GC-MS)BotanyQK1-989ENPlants, Vol 10, Iss 2404, p 2404 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Taiwania cryptomerioides</i> monoterpene synthases gas chromatography-mass spectrometry (GC-MS) Botany QK1-989
spellingShingle	<i>Taiwania cryptomerioides</i> monoterpene synthases gas chromatography-mass spectrometry (GC-MS) Botany QK1-989 Li-Ting Ma Pi-Ling Liu Yang-Tui Cheng Tz-Fan Shiu Fang-Hua Chu Unveiling Monoterpene Biosynthesis in <i>Taiwania cryptomerioides</i> via Functional Characterization
description	<i>Taiwania cryptomerioides</i> is a monotypic species, and its terpenoid-rich property has been reported in recent years. To uncover monoterpene biosynthesis in <i>T. cryptomerioides</i>, this study used transcriptome mining to identify candidates with tentative monoterpene synthase activity. Along with the phylogenetic analysis and in vitro assay, two geraniol synthases (<i>Tc</i>TPS13 and <i>Tc</i>TPS14), a linalool synthase (<i>Tc</i>TPS15), and a β-pinene synthase (<i>Tc</i>TPS16), were functionally characterized. Via the comparison of catalytic residues, the Cys/Ser at region 1 might be crucial in determining the formation of α-pinene or β-pinene. In addition, the Cupressaceae monoterpene synthases were phylogenetically clustered together; they are unique and different from those of published conifer species. In summary, this study aimed to uncover the ambiguous monoterpenoid network in <i>T. cryptomerioide</i>, which would expand the landscape of monoterpene biosynthesis in Cupressaceae species.
format	article
author	Li-Ting Ma Pi-Ling Liu Yang-Tui Cheng Tz-Fan Shiu Fang-Hua Chu
author_facet	Li-Ting Ma Pi-Ling Liu Yang-Tui Cheng Tz-Fan Shiu Fang-Hua Chu
author_sort	Li-Ting Ma
title	Unveiling Monoterpene Biosynthesis in <i>Taiwania cryptomerioides</i> via Functional Characterization
title_short	Unveiling Monoterpene Biosynthesis in <i>Taiwania cryptomerioides</i> via Functional Characterization
title_full	Unveiling Monoterpene Biosynthesis in <i>Taiwania cryptomerioides</i> via Functional Characterization
title_fullStr	Unveiling Monoterpene Biosynthesis in <i>Taiwania cryptomerioides</i> via Functional Characterization
title_full_unstemmed	Unveiling Monoterpene Biosynthesis in <i>Taiwania cryptomerioides</i> via Functional Characterization
title_sort	unveiling monoterpene biosynthesis in <i>taiwania cryptomerioides</i> via functional characterization
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e80d5fd2cd184926951939b794ab708c
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Unveiling Monoterpene Biosynthesis in <i>Taiwania cryptomerioides</i> via Functional Characterization

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