Insights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities

Soil salinity causes severe environmental stress that affects agriculture production and food security throughout the world. Salt-tolerant plant-growth-promoting rhizobacteria (PGPR) and nitric oxide (NO), a distinctive signaling molecule, can synergistically assist in the alleviation of abiotic str...

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Autores principales:	Anjney Sharma, Rajesh Kumar Singh, Pratiksha Singh, Anukool Vaishnav, Dao-Jun Guo, Krishan K. Verma, Dong-Ping Li, Xiu-Peng Song, Mukesh Kumar Malviya, Naeem Khan, Prakash Lakshmanan, Yang-Rui Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	soil salinity PGPR sodium nitroprusside synergistic effects reactive oxygen species antioxidant enzyme Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e80e4cdf75c24e92aae63ba6000e9f18
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spelling	oai:doaj.org-article:e80e4cdf75c24e92aae63ba6000e9f182021-11-25T18:24:13ZInsights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities10.3390/microorganisms91122032076-2607https://doaj.org/article/e80e4cdf75c24e92aae63ba6000e9f182021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2203https://doaj.org/toc/2076-2607Soil salinity causes severe environmental stress that affects agriculture production and food security throughout the world. Salt-tolerant plant-growth-promoting rhizobacteria (PGPR) and nitric oxide (NO), a distinctive signaling molecule, can synergistically assist in the alleviation of abiotic stresses and plant growth promotion, but the mechanism by which this happens is still not well known. In the present study, in a potential salt-tolerant rhizobacteria strain, ASN-1, growth up to 15% NaCl concentration was achieved with sugarcane rhizosphere soil. Based on 16S-rRNA gene sequencing analysis, the strain ASN-1 was identified as a <i>Bacillus xiamenensis</i>. Strain ASN-1 exhibits multiple plant-growth-promoting attributes, such as the production of indole-3-acetic acid, 1-aminocyclopropane-1-carboxylate deaminase, siderophores, HCN, ammonia, and exopolysaccharides as well as solubilized phosphate solubilization. Biofilm formation showed that NO enhanced the biofilm and root colonization capacity of the PGPR strain ASN-1 with host plants, evidenced by scanning electron microscopy. The greenhouse study showed that, among the different treatments, the combined application of PGPR and sodium nitroprusside (SNP) as an NO donor significantly (<i>p</i> ≤ 0.05) enhanced sugarcane plant growth by maintaining the relative water content, electrolyte leakage, gas exchange parameters, osmolytes, and Na<sup>+</sup>/K<sup>+</sup> ratio. Furthermore, PGPR and SNP fertilization reduced the salinity-induced oxidative stress in plants by modulating the antioxidant enzyme activities and stress-related gene expression. Thus, it is believed that the acquisition of advanced information about the synergistic effect of salt-tolerant PGPR and NO fertilization will reduce the use of harmful chemicals and aid in eco-friendly sustainable agricultural production under salt stress conditions.Anjney SharmaRajesh Kumar SinghPratiksha SinghAnukool VaishnavDao-Jun GuoKrishan K. VermaDong-Ping LiXiu-Peng SongMukesh Kumar MalviyaNaeem KhanPrakash LakshmananYang-Rui LiMDPI AGarticlesoil salinityPGPRsodium nitroprussidesynergistic effectsreactive oxygen speciesantioxidant enzymeBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2203, p 2203 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	soil salinity PGPR sodium nitroprusside synergistic effects reactive oxygen species antioxidant enzyme Biology (General) QH301-705.5
spellingShingle	soil salinity PGPR sodium nitroprusside synergistic effects reactive oxygen species antioxidant enzyme Biology (General) QH301-705.5 Anjney Sharma Rajesh Kumar Singh Pratiksha Singh Anukool Vaishnav Dao-Jun Guo Krishan K. Verma Dong-Ping Li Xiu-Peng Song Mukesh Kumar Malviya Naeem Khan Prakash Lakshmanan Yang-Rui Li Insights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities
description	Soil salinity causes severe environmental stress that affects agriculture production and food security throughout the world. Salt-tolerant plant-growth-promoting rhizobacteria (PGPR) and nitric oxide (NO), a distinctive signaling molecule, can synergistically assist in the alleviation of abiotic stresses and plant growth promotion, but the mechanism by which this happens is still not well known. In the present study, in a potential salt-tolerant rhizobacteria strain, ASN-1, growth up to 15% NaCl concentration was achieved with sugarcane rhizosphere soil. Based on 16S-rRNA gene sequencing analysis, the strain ASN-1 was identified as a <i>Bacillus xiamenensis</i>. Strain ASN-1 exhibits multiple plant-growth-promoting attributes, such as the production of indole-3-acetic acid, 1-aminocyclopropane-1-carboxylate deaminase, siderophores, HCN, ammonia, and exopolysaccharides as well as solubilized phosphate solubilization. Biofilm formation showed that NO enhanced the biofilm and root colonization capacity of the PGPR strain ASN-1 with host plants, evidenced by scanning electron microscopy. The greenhouse study showed that, among the different treatments, the combined application of PGPR and sodium nitroprusside (SNP) as an NO donor significantly (<i>p</i> ≤ 0.05) enhanced sugarcane plant growth by maintaining the relative water content, electrolyte leakage, gas exchange parameters, osmolytes, and Na<sup>+</sup>/K<sup>+</sup> ratio. Furthermore, PGPR and SNP fertilization reduced the salinity-induced oxidative stress in plants by modulating the antioxidant enzyme activities and stress-related gene expression. Thus, it is believed that the acquisition of advanced information about the synergistic effect of salt-tolerant PGPR and NO fertilization will reduce the use of harmful chemicals and aid in eco-friendly sustainable agricultural production under salt stress conditions.
format	article
author	Anjney Sharma Rajesh Kumar Singh Pratiksha Singh Anukool Vaishnav Dao-Jun Guo Krishan K. Verma Dong-Ping Li Xiu-Peng Song Mukesh Kumar Malviya Naeem Khan Prakash Lakshmanan Yang-Rui Li
author_facet	Anjney Sharma Rajesh Kumar Singh Pratiksha Singh Anukool Vaishnav Dao-Jun Guo Krishan K. Verma Dong-Ping Li Xiu-Peng Song Mukesh Kumar Malviya Naeem Khan Prakash Lakshmanan Yang-Rui Li
author_sort	Anjney Sharma
title	Insights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities
title_short	Insights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities
title_full	Insights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities
title_fullStr	Insights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities
title_full_unstemmed	Insights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities
title_sort	insights into the bacterial and nitric oxide-induced salt tolerance in sugarcane and their growth-promoting abilities
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e80e4cdf75c24e92aae63ba6000e9f18
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Insights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities

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