Insights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities

Soil salinity causes severe environmental stress that affects agriculture production and food security throughout the world. Salt-tolerant plant-growth-promoting rhizobacteria (PGPR) and nitric oxide (NO), a distinctive signaling molecule, can synergistically assist in the alleviation of abiotic str...

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Autores principales: Anjney Sharma, Rajesh Kumar Singh, Pratiksha Singh, Anukool Vaishnav, Dao-Jun Guo, Krishan K. Verma, Dong-Ping Li, Xiu-Peng Song, Mukesh Kumar Malviya, Naeem Khan, Prakash Lakshmanan, Yang-Rui Li
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spelling oai:doaj.org-article:e80e4cdf75c24e92aae63ba6000e9f182021-11-25T18:24:13ZInsights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities10.3390/microorganisms91122032076-2607https://doaj.org/article/e80e4cdf75c24e92aae63ba6000e9f182021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2203https://doaj.org/toc/2076-2607Soil salinity causes severe environmental stress that affects agriculture production and food security throughout the world. Salt-tolerant plant-growth-promoting rhizobacteria (PGPR) and nitric oxide (NO), a distinctive signaling molecule, can synergistically assist in the alleviation of abiotic stresses and plant growth promotion, but the mechanism by which this happens is still not well known. In the present study, in a potential salt-tolerant rhizobacteria strain, ASN-1, growth up to 15% NaCl concentration was achieved with sugarcane rhizosphere soil. Based on 16S-rRNA gene sequencing analysis, the strain ASN-1 was identified as a <i>Bacillus xiamenensis</i>. Strain ASN-1 exhibits multiple plant-growth-promoting attributes, such as the production of indole-3-acetic acid, 1-aminocyclopropane-1-carboxylate deaminase, siderophores, HCN, ammonia, and exopolysaccharides as well as solubilized phosphate solubilization. Biofilm formation showed that NO enhanced the biofilm and root colonization capacity of the PGPR strain ASN-1 with host plants, evidenced by scanning electron microscopy. The greenhouse study showed that, among the different treatments, the combined application of PGPR and sodium nitroprusside (SNP) as an NO donor significantly (<i>p</i> ≤ 0.05) enhanced sugarcane plant growth by maintaining the relative water content, electrolyte leakage, gas exchange parameters, osmolytes, and Na<sup>+</sup>/K<sup>+</sup> ratio. Furthermore, PGPR and SNP fertilization reduced the salinity-induced oxidative stress in plants by modulating the antioxidant enzyme activities and stress-related gene expression. Thus, it is believed that the acquisition of advanced information about the synergistic effect of salt-tolerant PGPR and NO fertilization will reduce the use of harmful chemicals and aid in eco-friendly sustainable agricultural production under salt stress conditions.Anjney SharmaRajesh Kumar SinghPratiksha SinghAnukool VaishnavDao-Jun GuoKrishan K. VermaDong-Ping LiXiu-Peng SongMukesh Kumar MalviyaNaeem KhanPrakash LakshmananYang-Rui LiMDPI AGarticlesoil salinityPGPRsodium nitroprussidesynergistic effectsreactive oxygen speciesantioxidant enzymeBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2203, p 2203 (2021)
institution DOAJ
collection DOAJ
language EN
topic soil salinity
PGPR
sodium nitroprusside
synergistic effects
reactive oxygen species
antioxidant enzyme
Biology (General)
QH301-705.5
spellingShingle soil salinity
PGPR
sodium nitroprusside
synergistic effects
reactive oxygen species
antioxidant enzyme
Biology (General)
QH301-705.5
Anjney Sharma
Rajesh Kumar Singh
Pratiksha Singh
Anukool Vaishnav
Dao-Jun Guo
Krishan K. Verma
Dong-Ping Li
Xiu-Peng Song
Mukesh Kumar Malviya
Naeem Khan
Prakash Lakshmanan
Yang-Rui Li
Insights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities
description Soil salinity causes severe environmental stress that affects agriculture production and food security throughout the world. Salt-tolerant plant-growth-promoting rhizobacteria (PGPR) and nitric oxide (NO), a distinctive signaling molecule, can synergistically assist in the alleviation of abiotic stresses and plant growth promotion, but the mechanism by which this happens is still not well known. In the present study, in a potential salt-tolerant rhizobacteria strain, ASN-1, growth up to 15% NaCl concentration was achieved with sugarcane rhizosphere soil. Based on 16S-rRNA gene sequencing analysis, the strain ASN-1 was identified as a <i>Bacillus xiamenensis</i>. Strain ASN-1 exhibits multiple plant-growth-promoting attributes, such as the production of indole-3-acetic acid, 1-aminocyclopropane-1-carboxylate deaminase, siderophores, HCN, ammonia, and exopolysaccharides as well as solubilized phosphate solubilization. Biofilm formation showed that NO enhanced the biofilm and root colonization capacity of the PGPR strain ASN-1 with host plants, evidenced by scanning electron microscopy. The greenhouse study showed that, among the different treatments, the combined application of PGPR and sodium nitroprusside (SNP) as an NO donor significantly (<i>p</i> ≤ 0.05) enhanced sugarcane plant growth by maintaining the relative water content, electrolyte leakage, gas exchange parameters, osmolytes, and Na<sup>+</sup>/K<sup>+</sup> ratio. Furthermore, PGPR and SNP fertilization reduced the salinity-induced oxidative stress in plants by modulating the antioxidant enzyme activities and stress-related gene expression. Thus, it is believed that the acquisition of advanced information about the synergistic effect of salt-tolerant PGPR and NO fertilization will reduce the use of harmful chemicals and aid in eco-friendly sustainable agricultural production under salt stress conditions.
format article
author Anjney Sharma
Rajesh Kumar Singh
Pratiksha Singh
Anukool Vaishnav
Dao-Jun Guo
Krishan K. Verma
Dong-Ping Li
Xiu-Peng Song
Mukesh Kumar Malviya
Naeem Khan
Prakash Lakshmanan
Yang-Rui Li
author_facet Anjney Sharma
Rajesh Kumar Singh
Pratiksha Singh
Anukool Vaishnav
Dao-Jun Guo
Krishan K. Verma
Dong-Ping Li
Xiu-Peng Song
Mukesh Kumar Malviya
Naeem Khan
Prakash Lakshmanan
Yang-Rui Li
author_sort Anjney Sharma
title Insights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities
title_short Insights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities
title_full Insights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities
title_fullStr Insights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities
title_full_unstemmed Insights into the Bacterial and Nitric Oxide-Induced Salt Tolerance in Sugarcane and Their Growth-Promoting Abilities
title_sort insights into the bacterial and nitric oxide-induced salt tolerance in sugarcane and their growth-promoting abilities
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/e80e4cdf75c24e92aae63ba6000e9f18
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