IN VITRO COMPARISON OF DISK DIFFUSION METHOD AND AGAR DILUTION METHOD FOR SENSITIVITY OF POLYMYXIN B AGAINST MULTI DRUG RESISTANT ACINETOBACTER BAUMANNII

Objective: To compare the in vitro disk diffusion method and agar dilution method for sensitivity of Polymyxin B against multi drug resistant Acinetobacter baumannii. Study Design: Comparative cross sectional. Place and Duration of Study: The study was carried out in the department of Microbio...

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Autores principales:	Fatima Sana, Luqman Satti, Gohar Zaman, Aamer Ikram, Adeel Hussain Gardezi, Muhammad Tahir Khadim
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2019
Materias:	agar dilution broth microdilution extensively drug resistant kirby bauer disk diffusion multi drug resistant Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e80f3fbf176e40bc8fe11d283fda608d
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spelling	oai:doaj.org-article:e80f3fbf176e40bc8fe11d283fda608d2021-11-09T03:01:41ZIN VITRO COMPARISON OF DISK DIFFUSION METHOD AND AGAR DILUTION METHOD FOR SENSITIVITY OF POLYMYXIN B AGAINST MULTI DRUG RESISTANT ACINETOBACTER BAUMANNII0030-96482411-8842https://doaj.org/article/e80f3fbf176e40bc8fe11d283fda608d2019-10-01T00:00:00Zhttps://www.pafmj.org/index.php/PAFMJ/article/view/3402/2377https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To compare the in vitro disk diffusion method and agar dilution method for sensitivity of Polymyxin B against multi drug resistant Acinetobacter baumannii. Study Design: Comparative cross sectional. Place and Duration of Study: The study was carried out in the department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi from 1st Dec 2016 to 30th Dec 2017. Methodology: Total 253 clinical specimens received from intensive care units with yielded growth of multidrug resistant Acinetobacter baumannii were evaluated for Polymyxin B susceptibility. Both Kirby bauer disk diffusion and agar dilution methods were compared with a reference method, broth microdilution. Results: Among 253 multidrug resistant isolates, 180 (71%) were extensively drug resistant and 221 (87%) were carbapenem resistant. Comparison of the disk diffusion and the MIC method by Agar dilution showed 100% correlation. 251 isolates were sensitive and 2 were resistant to Polymyxin B with MICs and disk zone diameters within the range recommended by the CLSI (2014-17). MIC 50 and MIC 90 of Polymyxin B were found to be 0.5 and 1μg/ml with 99.2% susceptibility. Conclusion: Both disk diffusion and agar dilution can be used together as initial screening methods in low income countries for susceptibility reporting of polymyxins. They are simple, reliable and economical. Also hetero resistance shown by some Acinetobacter baumanii strains can easily be picked up by agar dilution method.Fatima SanaLuqman SattiGohar ZamanAamer IkramAdeel Hussain GardeziMuhammad Tahir KhadimArmy Medical College Rawalpindiarticleagar dilutionbroth microdilutionextensively drug resistantkirby bauer disk diffusionmulti drug resistantMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 69, Iss 5, Pp 998-1003 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	agar dilution broth microdilution extensively drug resistant kirby bauer disk diffusion multi drug resistant Medicine R Medicine (General) R5-920
spellingShingle	agar dilution broth microdilution extensively drug resistant kirby bauer disk diffusion multi drug resistant Medicine R Medicine (General) R5-920 Fatima Sana Luqman Satti Gohar Zaman Aamer Ikram Adeel Hussain Gardezi Muhammad Tahir Khadim IN VITRO COMPARISON OF DISK DIFFUSION METHOD AND AGAR DILUTION METHOD FOR SENSITIVITY OF POLYMYXIN B AGAINST MULTI DRUG RESISTANT ACINETOBACTER BAUMANNII
description	Objective: To compare the in vitro disk diffusion method and agar dilution method for sensitivity of Polymyxin B against multi drug resistant Acinetobacter baumannii. Study Design: Comparative cross sectional. Place and Duration of Study: The study was carried out in the department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi from 1st Dec 2016 to 30th Dec 2017. Methodology: Total 253 clinical specimens received from intensive care units with yielded growth of multidrug resistant Acinetobacter baumannii were evaluated for Polymyxin B susceptibility. Both Kirby bauer disk diffusion and agar dilution methods were compared with a reference method, broth microdilution. Results: Among 253 multidrug resistant isolates, 180 (71%) were extensively drug resistant and 221 (87%) were carbapenem resistant. Comparison of the disk diffusion and the MIC method by Agar dilution showed 100% correlation. 251 isolates were sensitive and 2 were resistant to Polymyxin B with MICs and disk zone diameters within the range recommended by the CLSI (2014-17). MIC 50 and MIC 90 of Polymyxin B were found to be 0.5 and 1μg/ml with 99.2% susceptibility. Conclusion: Both disk diffusion and agar dilution can be used together as initial screening methods in low income countries for susceptibility reporting of polymyxins. They are simple, reliable and economical. Also hetero resistance shown by some Acinetobacter baumanii strains can easily be picked up by agar dilution method.
format	article
author	Fatima Sana Luqman Satti Gohar Zaman Aamer Ikram Adeel Hussain Gardezi Muhammad Tahir Khadim
author_facet	Fatima Sana Luqman Satti Gohar Zaman Aamer Ikram Adeel Hussain Gardezi Muhammad Tahir Khadim
author_sort	Fatima Sana
title	IN VITRO COMPARISON OF DISK DIFFUSION METHOD AND AGAR DILUTION METHOD FOR SENSITIVITY OF POLYMYXIN B AGAINST MULTI DRUG RESISTANT ACINETOBACTER BAUMANNII
title_short	IN VITRO COMPARISON OF DISK DIFFUSION METHOD AND AGAR DILUTION METHOD FOR SENSITIVITY OF POLYMYXIN B AGAINST MULTI DRUG RESISTANT ACINETOBACTER BAUMANNII
title_full	IN VITRO COMPARISON OF DISK DIFFUSION METHOD AND AGAR DILUTION METHOD FOR SENSITIVITY OF POLYMYXIN B AGAINST MULTI DRUG RESISTANT ACINETOBACTER BAUMANNII
title_fullStr	IN VITRO COMPARISON OF DISK DIFFUSION METHOD AND AGAR DILUTION METHOD FOR SENSITIVITY OF POLYMYXIN B AGAINST MULTI DRUG RESISTANT ACINETOBACTER BAUMANNII
title_full_unstemmed	IN VITRO COMPARISON OF DISK DIFFUSION METHOD AND AGAR DILUTION METHOD FOR SENSITIVITY OF POLYMYXIN B AGAINST MULTI DRUG RESISTANT ACINETOBACTER BAUMANNII
title_sort	in vitro comparison of disk diffusion method and agar dilution method for sensitivity of polymyxin b against multi drug resistant acinetobacter baumannii
publisher	Army Medical College Rawalpindi
publishDate	2019
url	https://doaj.org/article/e80f3fbf176e40bc8fe11d283fda608d
work_keys_str_mv	AT fatimasana invitrocomparisonofdiskdiffusionmethodandagardilutionmethodforsensitivityofpolymyxinbagainstmultidrugresistantacinetobacterbaumannii AT luqmansatti invitrocomparisonofdiskdiffusionmethodandagardilutionmethodforsensitivityofpolymyxinbagainstmultidrugresistantacinetobacterbaumannii AT goharzaman invitrocomparisonofdiskdiffusionmethodandagardilutionmethodforsensitivityofpolymyxinbagainstmultidrugresistantacinetobacterbaumannii AT aamerikram invitrocomparisonofdiskdiffusionmethodandagardilutionmethodforsensitivityofpolymyxinbagainstmultidrugresistantacinetobacterbaumannii AT adeelhussaingardezi invitrocomparisonofdiskdiffusionmethodandagardilutionmethodforsensitivityofpolymyxinbagainstmultidrugresistantacinetobacterbaumannii AT muhammadtahirkhadim invitrocomparisonofdiskdiffusionmethodandagardilutionmethodforsensitivityofpolymyxinbagainstmultidrugresistantacinetobacterbaumannii
_version_	1718441434008256512

IN VITRO COMPARISON OF DISK DIFFUSION METHOD AND AGAR DILUTION METHOD FOR SENSITIVITY OF POLYMYXIN B AGAINST MULTI DRUG RESISTANT ACINETOBACTER BAUMANNII

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