Identification and profiling of microRNAs from skeletal muscle of the common carp.

The common carp is one of the most important cultivated species in the world of freshwater aquaculture. The cultivation of this species is particularly productive due to its high skeletal muscle mass; however, the molecular mechanisms of skeletal muscle development in the common carp remain unknown....

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Autores principales:	Xuechun Yan, Lei Ding, Yunchao Li, Xiaofeng Zhang, Yang Liang, Xiaowen Sun, Chun-Bo Teng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e8117c39df5e49ca90af00bc4a713d5b
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spelling	oai:doaj.org-article:e8117c39df5e49ca90af00bc4a713d5b2021-11-18T07:29:09ZIdentification and profiling of microRNAs from skeletal muscle of the common carp.1932-620310.1371/journal.pone.0030925https://doaj.org/article/e8117c39df5e49ca90af00bc4a713d5b2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22303472/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The common carp is one of the most important cultivated species in the world of freshwater aquaculture. The cultivation of this species is particularly productive due to its high skeletal muscle mass; however, the molecular mechanisms of skeletal muscle development in the common carp remain unknown. It has been shown that a class of non-coding ∼22 nucleotide RNAs called microRNAs (miRNAs) play important roles in vertebrate development. They regulate gene expression through sequence-specific interactions with the 3' untranslated regions (UTRs) of target mRNAs and thereby cause translational repression or mRNA destabilization. Intriguingly, the role of miRNAs in the skeletal muscle development of the common carp remains unknown. In this study, a small-RNA cDNA library was constructed from the skeletal muscle of the common carp, and Solexa sequencing technology was used to perform high throughput sequencing of the library. Subsequent bioinformatics analysis identified 188 conserved miRNAs and 7 novel miRNAs in the carp skeletal muscle. The miRNA expression profiling showed that, miR-1, miR-133a-3p, and miR-206 were specifically expressed in muscle-containing organs, and that miR-1, miR-21, miR-26a, miR-27a, miR-133a-3p, miR-206, miR-214 and miR-222 were differentially expressed in the process of skeletal muscle development of the common carp. This study provides a first identification and profiling of miRNAs related to the muscle biology of the common carp. Their identification could provide clues leading towards a better understanding of the molecular mechanisms of carp skeletal muscle development.Xuechun YanLei DingYunchao LiXiaofeng ZhangYang LiangXiaowen SunChun-Bo TengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30925 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xuechun Yan Lei Ding Yunchao Li Xiaofeng Zhang Yang Liang Xiaowen Sun Chun-Bo Teng Identification and profiling of microRNAs from skeletal muscle of the common carp.
description	The common carp is one of the most important cultivated species in the world of freshwater aquaculture. The cultivation of this species is particularly productive due to its high skeletal muscle mass; however, the molecular mechanisms of skeletal muscle development in the common carp remain unknown. It has been shown that a class of non-coding ∼22 nucleotide RNAs called microRNAs (miRNAs) play important roles in vertebrate development. They regulate gene expression through sequence-specific interactions with the 3' untranslated regions (UTRs) of target mRNAs and thereby cause translational repression or mRNA destabilization. Intriguingly, the role of miRNAs in the skeletal muscle development of the common carp remains unknown. In this study, a small-RNA cDNA library was constructed from the skeletal muscle of the common carp, and Solexa sequencing technology was used to perform high throughput sequencing of the library. Subsequent bioinformatics analysis identified 188 conserved miRNAs and 7 novel miRNAs in the carp skeletal muscle. The miRNA expression profiling showed that, miR-1, miR-133a-3p, and miR-206 were specifically expressed in muscle-containing organs, and that miR-1, miR-21, miR-26a, miR-27a, miR-133a-3p, miR-206, miR-214 and miR-222 were differentially expressed in the process of skeletal muscle development of the common carp. This study provides a first identification and profiling of miRNAs related to the muscle biology of the common carp. Their identification could provide clues leading towards a better understanding of the molecular mechanisms of carp skeletal muscle development.
format	article
author	Xuechun Yan Lei Ding Yunchao Li Xiaofeng Zhang Yang Liang Xiaowen Sun Chun-Bo Teng
author_facet	Xuechun Yan Lei Ding Yunchao Li Xiaofeng Zhang Yang Liang Xiaowen Sun Chun-Bo Teng
author_sort	Xuechun Yan
title	Identification and profiling of microRNAs from skeletal muscle of the common carp.
title_short	Identification and profiling of microRNAs from skeletal muscle of the common carp.
title_full	Identification and profiling of microRNAs from skeletal muscle of the common carp.
title_fullStr	Identification and profiling of microRNAs from skeletal muscle of the common carp.
title_full_unstemmed	Identification and profiling of microRNAs from skeletal muscle of the common carp.
title_sort	identification and profiling of micrornas from skeletal muscle of the common carp.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e8117c39df5e49ca90af00bc4a713d5b
work_keys_str_mv	AT xuechunyan identificationandprofilingofmicrornasfromskeletalmuscleofthecommoncarp AT leiding identificationandprofilingofmicrornasfromskeletalmuscleofthecommoncarp AT yunchaoli identificationandprofilingofmicrornasfromskeletalmuscleofthecommoncarp AT xiaofengzhang identificationandprofilingofmicrornasfromskeletalmuscleofthecommoncarp AT yangliang identificationandprofilingofmicrornasfromskeletalmuscleofthecommoncarp AT xiaowensun identificationandprofilingofmicrornasfromskeletalmuscleofthecommoncarp AT chunboteng identificationandprofilingofmicrornasfromskeletalmuscleofthecommoncarp
_version_	1718423445210923008

Identification and profiling of microRNAs from skeletal muscle of the common carp.

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