PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS

Like proteins, antimicrobial peptides (AMP) are versatile molecules synthesized by microorganisms using enzymatic pathways with no genetic code instruction. AMP have interesting properties in the food and pharmaceutical industries, like their antimicrobial ability against pathogens. Looking for biom...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ligia L. CORRALES-GARCÍA, Gelmy L. CIRO G.
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2010
Materias:	antimicrobial cationic peptides soil microbiology thin layer chromatography Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/e812c59cd8204430ab3b4100c3c4aee0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e812c59cd8204430ab3b4100c3c4aee0
record_format	dspace
spelling	oai:doaj.org-article:e812c59cd8204430ab3b4100c3c4aee02021-11-19T04:14:23ZPEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS0121-40042145-2660https://doaj.org/article/e812c59cd8204430ab3b4100c3c4aee02010-07-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/6343https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660Like proteins, antimicrobial peptides (AMP) are versatile molecules synthesized by microorganisms using enzymatic pathways with no genetic code instruction. AMP have interesting properties in the food and pharmaceutical industries, like their antimicrobial ability against pathogens. Looking for biomolecules from microorganisms requires hard and time consuming chemical analysis of each microorganism extract. The microorganism isolation method proposed in this research allowed us to find antimicrobial peptides produced by bacteria, through interaction between a charged dye mixed with selective agar and metabolites produced by microorganisms. Twenty soil samples from different zones were isolated in selective media; thirty five strains were purified based on interaction between basic dye and charged molecules from bacteria. Streptomyces sp. y Bacillus sp. both genera were identified. Protein extracts were obtained from the isolated microorganisms cultivated in liquid media; peptides and amino acids were identified by thin layer chromatography and electrophoresis. Those extracts with high protein level were used to evaluate bioautography. Two extracts from 35 showed inhibitory activity against E. coli ATCC 8739 (8 mm halo). Method effectiveness for the isolation and the purifying of microorganisms able to produce charged molecules, of industrial interest is demonstrated.Ligia L. CORRALES-GARCÍAGelmy L. CIRO G.Universidad de Antioquiaarticleantimicrobial cationic peptidessoil microbiologythin layer chromatographyFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 17, Iss 2 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial cationic peptides soil microbiology thin layer chromatography Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	antimicrobial cationic peptides soil microbiology thin layer chromatography Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Ligia L. CORRALES-GARCÍA Gelmy L. CIRO G. PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS
description	Like proteins, antimicrobial peptides (AMP) are versatile molecules synthesized by microorganisms using enzymatic pathways with no genetic code instruction. AMP have interesting properties in the food and pharmaceutical industries, like their antimicrobial ability against pathogens. Looking for biomolecules from microorganisms requires hard and time consuming chemical analysis of each microorganism extract. The microorganism isolation method proposed in this research allowed us to find antimicrobial peptides produced by bacteria, through interaction between a charged dye mixed with selective agar and metabolites produced by microorganisms. Twenty soil samples from different zones were isolated in selective media; thirty five strains were purified based on interaction between basic dye and charged molecules from bacteria. Streptomyces sp. y Bacillus sp. both genera were identified. Protein extracts were obtained from the isolated microorganisms cultivated in liquid media; peptides and amino acids were identified by thin layer chromatography and electrophoresis. Those extracts with high protein level were used to evaluate bioautography. Two extracts from 35 showed inhibitory activity against E. coli ATCC 8739 (8 mm halo). Method effectiveness for the isolation and the purifying of microorganisms able to produce charged molecules, of industrial interest is demonstrated.
format	article
author	Ligia L. CORRALES-GARCÍA Gelmy L. CIRO G.
author_facet	Ligia L. CORRALES-GARCÍA Gelmy L. CIRO G.
author_sort	Ligia L. CORRALES-GARCÍA
title	PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS
title_short	PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS
title_full	PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS
title_fullStr	PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS
title_full_unstemmed	PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS
title_sort	peptides with antimicrobial activity produced by isolated native microorganisms
publisher	Universidad de Antioquia
publishDate	2010
url	https://doaj.org/article/e812c59cd8204430ab3b4100c3c4aee0
work_keys_str_mv	AT ligialcorralesgarcia peptideswithantimicrobialactivityproducedbyisolatednativemicroorganisms AT gelmylcirog peptideswithantimicrobialactivityproducedbyisolatednativemicroorganisms
_version_	1718420470416539648

PEPTIDES WITH ANTIMICROBIAL ACTIVITY PRODUCED BY ISOLATED NATIVE MICROORGANISMS

Ejemplares similares