Poroelastic osmoregulation of living cell volume

Summary: Cells maintain their volume through fine intracellular osmolarity regulation. Osmotic challenges drive fluid into or out of cells causing swelling or shrinkage, respectively. The dynamics of cell volume changes depending on the rheology of the cellular constituents and on how fast the fluid...

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Autores principales:	Mohammad Hadi Esteki, Andrea Malandrino, Ali Akbar Alemrajabi, Graham K. Sheridan, Guillaume Charras, Emad Moeendarbary
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cellular physiology Cell biology Biomechanics Science Q
Acceso en línea:	https://doaj.org/article/e823ba71f26d4d998080e1a96b59c551
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spelling	oai:doaj.org-article:e823ba71f26d4d998080e1a96b59c5512021-12-04T04:35:34ZPoroelastic osmoregulation of living cell volume2589-004210.1016/j.isci.2021.103482https://doaj.org/article/e823ba71f26d4d998080e1a96b59c5512021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S258900422101453Xhttps://doaj.org/toc/2589-0042Summary: Cells maintain their volume through fine intracellular osmolarity regulation. Osmotic challenges drive fluid into or out of cells causing swelling or shrinkage, respectively. The dynamics of cell volume changes depending on the rheology of the cellular constituents and on how fast the fluid permeates through the membrane and cytoplasm. We investigated whether and how poroelasticity can describe volume dynamics in response to osmotic shocks. We exposed cells to osmotic perturbations and used defocusing epifluorescence microscopy on membrane-attached fluorescent nanospheres to track volume dynamics with high spatiotemporal resolution. We found that a poroelastic model that considers both geometrical and pressurization rates captures fluid-cytoskeleton interactions, which are rate-limiting factors in controlling volume changes at short timescales. Linking cellular responses to osmotic shocks and cell mechanics through poroelasticity can predict the cell state in health, disease, or in response to novel therapeutics.Mohammad Hadi EstekiAndrea MalandrinoAli Akbar AlemrajabiGraham K. SheridanGuillaume CharrasEmad MoeendarbaryElsevierarticleCellular physiologyCell biologyBiomechanicsScienceQENiScience, Vol 24, Iss 12, Pp 103482- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cellular physiology Cell biology Biomechanics Science Q
spellingShingle	Cellular physiology Cell biology Biomechanics Science Q Mohammad Hadi Esteki Andrea Malandrino Ali Akbar Alemrajabi Graham K. Sheridan Guillaume Charras Emad Moeendarbary Poroelastic osmoregulation of living cell volume
description	Summary: Cells maintain their volume through fine intracellular osmolarity regulation. Osmotic challenges drive fluid into or out of cells causing swelling or shrinkage, respectively. The dynamics of cell volume changes depending on the rheology of the cellular constituents and on how fast the fluid permeates through the membrane and cytoplasm. We investigated whether and how poroelasticity can describe volume dynamics in response to osmotic shocks. We exposed cells to osmotic perturbations and used defocusing epifluorescence microscopy on membrane-attached fluorescent nanospheres to track volume dynamics with high spatiotemporal resolution. We found that a poroelastic model that considers both geometrical and pressurization rates captures fluid-cytoskeleton interactions, which are rate-limiting factors in controlling volume changes at short timescales. Linking cellular responses to osmotic shocks and cell mechanics through poroelasticity can predict the cell state in health, disease, or in response to novel therapeutics.
format	article
author	Mohammad Hadi Esteki Andrea Malandrino Ali Akbar Alemrajabi Graham K. Sheridan Guillaume Charras Emad Moeendarbary
author_facet	Mohammad Hadi Esteki Andrea Malandrino Ali Akbar Alemrajabi Graham K. Sheridan Guillaume Charras Emad Moeendarbary
author_sort	Mohammad Hadi Esteki
title	Poroelastic osmoregulation of living cell volume
title_short	Poroelastic osmoregulation of living cell volume
title_full	Poroelastic osmoregulation of living cell volume
title_fullStr	Poroelastic osmoregulation of living cell volume
title_full_unstemmed	Poroelastic osmoregulation of living cell volume
title_sort	poroelastic osmoregulation of living cell volume
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e823ba71f26d4d998080e1a96b59c551
work_keys_str_mv	AT mohammadhadiesteki poroelasticosmoregulationoflivingcellvolume AT andreamalandrino poroelasticosmoregulationoflivingcellvolume AT aliakbaralemrajabi poroelasticosmoregulationoflivingcellvolume AT grahamksheridan poroelasticosmoregulationoflivingcellvolume AT guillaumecharras poroelasticosmoregulationoflivingcellvolume AT emadmoeendarbary poroelasticosmoregulationoflivingcellvolume
_version_	1718372945631379456

Poroelastic osmoregulation of living cell volume

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