Efficacy of two primer sets used in sex identification of rufous-winged buzzard (Butastur liventer)

Background: The rufous-winged buzzard (Butastur liventer) belongs to the Accipitriformes, which are monomorphic, resulting in difficulty to identify the gender. However, sex determination is important for predicting the sex ratio this buzzard in nature in order to avoid its extinction. Aim: We aime...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Paitoon Kaewhom, Kanokrat Srikijkasemwat
Formato:	article
Lenguaje:	EN
Publicado:	Tripoli University 2021
Materias:	chd gene pcr technique gender identification birds Zoology QL1-991
Acceso en línea:	https://doaj.org/article/e826910026064342a7c65b840b53d8e7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e826910026064342a7c65b840b53d8e7
record_format	dspace
spelling	oai:doaj.org-article:e826910026064342a7c65b840b53d8e72021-11-06T19:24:53ZEfficacy of two primer sets used in sex identification of rufous-winged buzzard (Butastur liventer)10.5455/OVJ.2021.v11.i4.72226-44852218-6050https://doaj.org/article/e826910026064342a7c65b840b53d8e72021-10-01T00:00:00Zhttps://www.openveterinaryjournal.com/OVJ-2021-06-122%20P.%20Kaewhom%20and%20K.%20Srikijkasemwat.pdfhttps://doaj.org/toc/2226-4485https://doaj.org/toc/2218-6050Background: The rufous-winged buzzard (Butastur liventer) belongs to the Accipitriformes, which are monomorphic, resulting in difficulty to identify the gender. However, sex determination is important for predicting the sex ratio this buzzard in nature in order to avoid its extinction. Aim: We aimed to develop a primer set, able to sex the rufous-winged buzzard through PCR amplification, and compare the efficacy of two sets of primers by using PCR technique. Methods: In the following, sensitivity refers to the smallest DNA concentration that allowed us to accurately sex a bird and specificity refers to the ability to clearly distinguish the sex based on visual appearance of the bands. Blood samples were collected from captive buzzards. The DNA was extracted from them and was diluted to 50, 25, 10, 5, 2.5, 1.67 and 1 ng/µL. Two sets of primers, including P2/NP/MP and 2550F/2718R, were used to amplify the CHD gene of known gender buzzards usingthe PCR process to determine gender and to compare their sensitivity. To measure specificity, both primers were used to amplify CHD gene fragment of other unknown gender birds. Results: The lowest concentration of the DNA template that P2/NP/MP could amplify DNA fragments was 1 ng/µL, and this set of primers could identify the gender of all birdscorrectly, giving 100% specificity. On the other hand, the 2550F/2718R could amplify the DNA fragments from 5 ng/µL, and it had only 78% specificity. Conclusion: The P2/NP/MP primer set was able to correctly identify the gender of rufous-winged buzzard through PCR amplification with high specificity and sensitivity.Paitoon KaewhomKanokrat SrikijkasemwatTripoli Universityarticlechd genepcr techniquegender identificationbirdsZoologyQL1-991ENOpen Veterinary Journal, Vol 11, Iss 4, Pp 581-586 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chd gene pcr technique gender identification birds Zoology QL1-991
spellingShingle	chd gene pcr technique gender identification birds Zoology QL1-991 Paitoon Kaewhom Kanokrat Srikijkasemwat Efficacy of two primer sets used in sex identification of rufous-winged buzzard (Butastur liventer)
description	Background: The rufous-winged buzzard (Butastur liventer) belongs to the Accipitriformes, which are monomorphic, resulting in difficulty to identify the gender. However, sex determination is important for predicting the sex ratio this buzzard in nature in order to avoid its extinction. Aim: We aimed to develop a primer set, able to sex the rufous-winged buzzard through PCR amplification, and compare the efficacy of two sets of primers by using PCR technique. Methods: In the following, sensitivity refers to the smallest DNA concentration that allowed us to accurately sex a bird and specificity refers to the ability to clearly distinguish the sex based on visual appearance of the bands. Blood samples were collected from captive buzzards. The DNA was extracted from them and was diluted to 50, 25, 10, 5, 2.5, 1.67 and 1 ng/µL. Two sets of primers, including P2/NP/MP and 2550F/2718R, were used to amplify the CHD gene of known gender buzzards usingthe PCR process to determine gender and to compare their sensitivity. To measure specificity, both primers were used to amplify CHD gene fragment of other unknown gender birds. Results: The lowest concentration of the DNA template that P2/NP/MP could amplify DNA fragments was 1 ng/µL, and this set of primers could identify the gender of all birdscorrectly, giving 100% specificity. On the other hand, the 2550F/2718R could amplify the DNA fragments from 5 ng/µL, and it had only 78% specificity. Conclusion: The P2/NP/MP primer set was able to correctly identify the gender of rufous-winged buzzard through PCR amplification with high specificity and sensitivity.
format	article
author	Paitoon Kaewhom Kanokrat Srikijkasemwat
author_facet	Paitoon Kaewhom Kanokrat Srikijkasemwat
author_sort	Paitoon Kaewhom
title	Efficacy of two primer sets used in sex identification of rufous-winged buzzard (Butastur liventer)
title_short	Efficacy of two primer sets used in sex identification of rufous-winged buzzard (Butastur liventer)
title_full	Efficacy of two primer sets used in sex identification of rufous-winged buzzard (Butastur liventer)
title_fullStr	Efficacy of two primer sets used in sex identification of rufous-winged buzzard (Butastur liventer)
title_full_unstemmed	Efficacy of two primer sets used in sex identification of rufous-winged buzzard (Butastur liventer)
title_sort	efficacy of two primer sets used in sex identification of rufous-winged buzzard (butastur liventer)
publisher	Tripoli University
publishDate	2021
url	https://doaj.org/article/e826910026064342a7c65b840b53d8e7
work_keys_str_mv	AT paitoonkaewhom efficacyoftwoprimersetsusedinsexidentificationofrufouswingedbuzzardbutasturliventer AT kanokratsrikijkasemwat efficacyoftwoprimersetsusedinsexidentificationofrufouswingedbuzzardbutasturliventer
_version_	1718443704395497472

Efficacy of two primer sets used in sex identification of rufous-winged buzzard (Butastur liventer)

Ejemplares similares