Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells

Abstract The aim of the present study was to investigate combined effects of cold atmospheric plasma (CAP) and the chemotherapeutic drug doxorubicin (DOX) on murine and human melanoma cells, and normal cells. In addition to free drug, the combination of CAP with a liposomal drug (DOX-LIP) was also s...

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Autores principales:	Konstantina Pefani-Antimisiari, Dimitrios K. Athanasopoulos, Antonia Marazioti, Kyriakos Sklias, Maria Rodi, Anne-Lise de Lastic, Athanasia Mouzaki, Panagiotis Svarnas, Sophia G. Antimisiaris
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e82801683e1e44839d35fe7c5f35fbef
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spelling	oai:doaj.org-article:e82801683e1e44839d35fe7c5f35fbef2021-12-02T16:26:38ZSynergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells10.1038/s41598-021-94130-72045-2322https://doaj.org/article/e82801683e1e44839d35fe7c5f35fbef2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94130-7https://doaj.org/toc/2045-2322Abstract The aim of the present study was to investigate combined effects of cold atmospheric plasma (CAP) and the chemotherapeutic drug doxorubicin (DOX) on murine and human melanoma cells, and normal cells. In addition to free drug, the combination of CAP with a liposomal drug (DOX-LIP) was also studied for the first time. Thiazolyl blue tetrazolium bromide (MTT) and Trypan Blue exclusion assays were used to evaluate cell viability; the mechanism of cell death was evaluated by flow cytometry. Combined treatment effects on the clonogenic capability of melanoma cells, was also tested with soft agar colony formation assay. Furthermore the effect of CAP on the cellular uptake of DOX or DOX-LIP was examined. Results showed a strong synergistic effect of CAP and DOX or DOX-LIP on selectively decreasing cell viability of melanoma cells. CAP accelerated the apoptotic effect of DOX (or DOX-LIP) and dramatically reduced the aggressiveness of melanoma cells, as the combination treatment significantly decreased their anchorage independent growth. Moreover, CAP did not result in increased cellular uptake of DOX under the present experimental conditions. In conclusion, CAP facilitates DOX cytotoxic effects on melanoma cells, and affects their metastatic potential by reducing their clonogenicity, as shown for the first time.Konstantina Pefani-AntimisiariDimitrios K. AthanasopoulosAntonia MaraziotiKyriakos SkliasMaria RodiAnne-Lise de LasticAthanasia MouzakiPanagiotis SvarnasSophia G. AntimisiarisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Konstantina Pefani-Antimisiari Dimitrios K. Athanasopoulos Antonia Marazioti Kyriakos Sklias Maria Rodi Anne-Lise de Lastic Athanasia Mouzaki Panagiotis Svarnas Sophia G. Antimisiaris Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells
description	Abstract The aim of the present study was to investigate combined effects of cold atmospheric plasma (CAP) and the chemotherapeutic drug doxorubicin (DOX) on murine and human melanoma cells, and normal cells. In addition to free drug, the combination of CAP with a liposomal drug (DOX-LIP) was also studied for the first time. Thiazolyl blue tetrazolium bromide (MTT) and Trypan Blue exclusion assays were used to evaluate cell viability; the mechanism of cell death was evaluated by flow cytometry. Combined treatment effects on the clonogenic capability of melanoma cells, was also tested with soft agar colony formation assay. Furthermore the effect of CAP on the cellular uptake of DOX or DOX-LIP was examined. Results showed a strong synergistic effect of CAP and DOX or DOX-LIP on selectively decreasing cell viability of melanoma cells. CAP accelerated the apoptotic effect of DOX (or DOX-LIP) and dramatically reduced the aggressiveness of melanoma cells, as the combination treatment significantly decreased their anchorage independent growth. Moreover, CAP did not result in increased cellular uptake of DOX under the present experimental conditions. In conclusion, CAP facilitates DOX cytotoxic effects on melanoma cells, and affects their metastatic potential by reducing their clonogenicity, as shown for the first time.
format	article
author	Konstantina Pefani-Antimisiari Dimitrios K. Athanasopoulos Antonia Marazioti Kyriakos Sklias Maria Rodi Anne-Lise de Lastic Athanasia Mouzaki Panagiotis Svarnas Sophia G. Antimisiaris
author_facet	Konstantina Pefani-Antimisiari Dimitrios K. Athanasopoulos Antonia Marazioti Kyriakos Sklias Maria Rodi Anne-Lise de Lastic Athanasia Mouzaki Panagiotis Svarnas Sophia G. Antimisiaris
author_sort	Konstantina Pefani-Antimisiari
title	Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells
title_short	Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells
title_full	Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells
title_fullStr	Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells
title_full_unstemmed	Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells
title_sort	synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e82801683e1e44839d35fe7c5f35fbef
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Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells

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