Diagnostic performance of the Abbott RealTime MTB assay for tuberculosis diagnosis in people living with HIV

Abstract Strengthening tuberculosis diagnosis is an international priority and the advocacy for multi-disease testing devices raises the possibility of improving laboratory efficiency. However, the advantages of centralized platforms might not translate into real improvements under operational condi...

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Autores principales:	Belén Saavedra, Edson Mambuque, Neide Gomes, Dinis Nguenha, Rita Mabunda, Luis Faife, Ruben Langa, Shilzia Munguambe, Filomena Manjate, Anelsio Cossa, Lesley Scott, Alberto L. García-Basteiro
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e8325a6f6b9747cfaba20c26e2062be4
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spelling	oai:doaj.org-article:e8325a6f6b9747cfaba20c26e2062be42021-12-02T18:51:28ZDiagnostic performance of the Abbott RealTime MTB assay for tuberculosis diagnosis in people living with HIV10.1038/s41598-021-96922-32045-2322https://doaj.org/article/e8325a6f6b9747cfaba20c26e2062be42021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96922-3https://doaj.org/toc/2045-2322Abstract Strengthening tuberculosis diagnosis is an international priority and the advocacy for multi-disease testing devices raises the possibility of improving laboratory efficiency. However, the advantages of centralized platforms might not translate into real improvements under operational conditions. This study aimed to evaluate the field use of the Abbott RealTime MTB (RT-MTB) and Xpert MTB/RIF assays, in a large cohort of HIV-positive and TB presumptive cases in Southern Mozambique. Over a 6-month period, 255 HIV-positive TB presumptive cases were consecutively recruited in the high TB/HIV burden district of Manhiça. The diagnostic performance of both assays was evaluated against two different reference standards: a microbiological gold standard (MGS) and a composite reference standard (CRS). Results from the primary analysis (MGS) showed improved sensitivity (Se) and reduced specificity (Sp) for the Abbott RT-MTB assay compared to the Xpert MTB/RIF (RT-MTB Se: 0.92 (95% CI: 0.75;0.99) vs Xpert Se: 0.73 (95% CI: 0.52;0.88) p value = 0.06; RT-MTB Sp: 0.80 (0.72;0.86) vs Xpert Sp: 0.96 (0.92;0.99) p value < 0.001). The lower specificity may be due to cross-reactivity with non-tuberculous mycobacteria (NTMs), the detection of non-viable MTBC, or the identification of true TB cases missed by the gold standard.Belén SaavedraEdson MambuqueNeide GomesDinis NguenhaRita MabundaLuis FaifeRuben LangaShilzia MunguambeFilomena ManjateAnelsio CossaLesley ScottAlberto L. García-BasteiroNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Belén Saavedra Edson Mambuque Neide Gomes Dinis Nguenha Rita Mabunda Luis Faife Ruben Langa Shilzia Munguambe Filomena Manjate Anelsio Cossa Lesley Scott Alberto L. García-Basteiro Diagnostic performance of the Abbott RealTime MTB assay for tuberculosis diagnosis in people living with HIV
description	Abstract Strengthening tuberculosis diagnosis is an international priority and the advocacy for multi-disease testing devices raises the possibility of improving laboratory efficiency. However, the advantages of centralized platforms might not translate into real improvements under operational conditions. This study aimed to evaluate the field use of the Abbott RealTime MTB (RT-MTB) and Xpert MTB/RIF assays, in a large cohort of HIV-positive and TB presumptive cases in Southern Mozambique. Over a 6-month period, 255 HIV-positive TB presumptive cases were consecutively recruited in the high TB/HIV burden district of Manhiça. The diagnostic performance of both assays was evaluated against two different reference standards: a microbiological gold standard (MGS) and a composite reference standard (CRS). Results from the primary analysis (MGS) showed improved sensitivity (Se) and reduced specificity (Sp) for the Abbott RT-MTB assay compared to the Xpert MTB/RIF (RT-MTB Se: 0.92 (95% CI: 0.75;0.99) vs Xpert Se: 0.73 (95% CI: 0.52;0.88) p value = 0.06; RT-MTB Sp: 0.80 (0.72;0.86) vs Xpert Sp: 0.96 (0.92;0.99) p value < 0.001). The lower specificity may be due to cross-reactivity with non-tuberculous mycobacteria (NTMs), the detection of non-viable MTBC, or the identification of true TB cases missed by the gold standard.
format	article
author	Belén Saavedra Edson Mambuque Neide Gomes Dinis Nguenha Rita Mabunda Luis Faife Ruben Langa Shilzia Munguambe Filomena Manjate Anelsio Cossa Lesley Scott Alberto L. García-Basteiro
author_facet	Belén Saavedra Edson Mambuque Neide Gomes Dinis Nguenha Rita Mabunda Luis Faife Ruben Langa Shilzia Munguambe Filomena Manjate Anelsio Cossa Lesley Scott Alberto L. García-Basteiro
author_sort	Belén Saavedra
title	Diagnostic performance of the Abbott RealTime MTB assay for tuberculosis diagnosis in people living with HIV
title_short	Diagnostic performance of the Abbott RealTime MTB assay for tuberculosis diagnosis in people living with HIV
title_full	Diagnostic performance of the Abbott RealTime MTB assay for tuberculosis diagnosis in people living with HIV
title_fullStr	Diagnostic performance of the Abbott RealTime MTB assay for tuberculosis diagnosis in people living with HIV
title_full_unstemmed	Diagnostic performance of the Abbott RealTime MTB assay for tuberculosis diagnosis in people living with HIV
title_sort	diagnostic performance of the abbott realtime mtb assay for tuberculosis diagnosis in people living with hiv
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e8325a6f6b9747cfaba20c26e2062be4
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Diagnostic performance of the Abbott RealTime MTB assay for tuberculosis diagnosis in people living with HIV

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