Osteocyte Apoptosis Contributes to Cold Exposure-induced Bone Loss

Emerging evidence indicates that bone mass is regulated by systemic energy balance. Temperature variations have profound effects on energy metabolism in animals, which will affect bone remodeling. But the mechanism remains unclear. 2-month-old C57BL/6J male mice were exposed to cold (4°C) and normal...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jingke Du, Zihao He, Junqi Cui, Hanjun Li, Mingming Xu, Shuhong Zhang, Shuangyan Zhang, Mengning Yan, Xinhua Qu, Zhifeng Yu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	cold exposure bone mass bone remodeling osteocyte perilacunar /canalicular remodeling Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/e8337e55a4b44699a4a04c1bb73d73b0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e8337e55a4b44699a4a04c1bb73d73b0
record_format	dspace
spelling	oai:doaj.org-article:e8337e55a4b44699a4a04c1bb73d73b02021-11-11T06:42:07ZOsteocyte Apoptosis Contributes to Cold Exposure-induced Bone Loss2296-418510.3389/fbioe.2021.733582https://doaj.org/article/e8337e55a4b44699a4a04c1bb73d73b02021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.733582/fullhttps://doaj.org/toc/2296-4185Emerging evidence indicates that bone mass is regulated by systemic energy balance. Temperature variations have profound effects on energy metabolism in animals, which will affect bone remodeling. But the mechanism remains unclear. 2-month-old C57BL/6J male mice were exposed to cold (4°C) and normal (23°C) temperatures for 28 days and the effects of cold exposure on bone mass was investigated. Micro-computed tomography results showed that bone volume fraction was significantly reduced after 14 days of exposure to cold temperature, and it was recovered after 28 days. Ploton silver staining and immunohistochemical results further revealed that exposure to cold decreased canalicular length, number of E11-and MMP13-positive osteocytes after 14 days, but they returned to the baseline levels after 28 days, different from the normal temperature control group. In addition, change of Caspase-3 indicated that exposure to cold temperature augmented apoptosis of osteocytes. In vitro results confirmed the positive effect of brown adipocytes on osteocyte‘s dendrites and E11 expression. In conclusion, our findings indicate that cold exposure can influence bone mass in a time-dependent manner, with bone mass decreasing and recovering at 2 and 4 weeks respectively. The change of bone mass may be caused by the apoptosis osteocytes. Brown adipocyte tissue could influence bone remodeling through affecting osteocyte.Jingke DuZihao HeJunqi CuiHanjun LiMingming XuShuhong ZhangShuangyan ZhangMengning YanXinhua QuZhifeng YuFrontiers Media S.A.articlecold exposurebone massbone remodelingosteocyteperilacunar /canalicular remodelingBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cold exposure bone mass bone remodeling osteocyte perilacunar /canalicular remodeling Biotechnology TP248.13-248.65
spellingShingle	cold exposure bone mass bone remodeling osteocyte perilacunar /canalicular remodeling Biotechnology TP248.13-248.65 Jingke Du Zihao He Junqi Cui Hanjun Li Mingming Xu Shuhong Zhang Shuangyan Zhang Mengning Yan Xinhua Qu Zhifeng Yu Osteocyte Apoptosis Contributes to Cold Exposure-induced Bone Loss
description	Emerging evidence indicates that bone mass is regulated by systemic energy balance. Temperature variations have profound effects on energy metabolism in animals, which will affect bone remodeling. But the mechanism remains unclear. 2-month-old C57BL/6J male mice were exposed to cold (4°C) and normal (23°C) temperatures for 28 days and the effects of cold exposure on bone mass was investigated. Micro-computed tomography results showed that bone volume fraction was significantly reduced after 14 days of exposure to cold temperature, and it was recovered after 28 days. Ploton silver staining and immunohistochemical results further revealed that exposure to cold decreased canalicular length, number of E11-and MMP13-positive osteocytes after 14 days, but they returned to the baseline levels after 28 days, different from the normal temperature control group. In addition, change of Caspase-3 indicated that exposure to cold temperature augmented apoptosis of osteocytes. In vitro results confirmed the positive effect of brown adipocytes on osteocyte‘s dendrites and E11 expression. In conclusion, our findings indicate that cold exposure can influence bone mass in a time-dependent manner, with bone mass decreasing and recovering at 2 and 4 weeks respectively. The change of bone mass may be caused by the apoptosis osteocytes. Brown adipocyte tissue could influence bone remodeling through affecting osteocyte.
format	article
author	Jingke Du Zihao He Junqi Cui Hanjun Li Mingming Xu Shuhong Zhang Shuangyan Zhang Mengning Yan Xinhua Qu Zhifeng Yu
author_facet	Jingke Du Zihao He Junqi Cui Hanjun Li Mingming Xu Shuhong Zhang Shuangyan Zhang Mengning Yan Xinhua Qu Zhifeng Yu
author_sort	Jingke Du
title	Osteocyte Apoptosis Contributes to Cold Exposure-induced Bone Loss
title_short	Osteocyte Apoptosis Contributes to Cold Exposure-induced Bone Loss
title_full	Osteocyte Apoptosis Contributes to Cold Exposure-induced Bone Loss
title_fullStr	Osteocyte Apoptosis Contributes to Cold Exposure-induced Bone Loss
title_full_unstemmed	Osteocyte Apoptosis Contributes to Cold Exposure-induced Bone Loss
title_sort	osteocyte apoptosis contributes to cold exposure-induced bone loss
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e8337e55a4b44699a4a04c1bb73d73b0
work_keys_str_mv	AT jingkedu osteocyteapoptosiscontributestocoldexposureinducedboneloss AT zihaohe osteocyteapoptosiscontributestocoldexposureinducedboneloss AT junqicui osteocyteapoptosiscontributestocoldexposureinducedboneloss AT hanjunli osteocyteapoptosiscontributestocoldexposureinducedboneloss AT mingmingxu osteocyteapoptosiscontributestocoldexposureinducedboneloss AT shuhongzhang osteocyteapoptosiscontributestocoldexposureinducedboneloss AT shuangyanzhang osteocyteapoptosiscontributestocoldexposureinducedboneloss AT mengningyan osteocyteapoptosiscontributestocoldexposureinducedboneloss AT xinhuaqu osteocyteapoptosiscontributestocoldexposureinducedboneloss AT zhifengyu osteocyteapoptosiscontributestocoldexposureinducedboneloss
_version_	1718439548038414336

Osteocyte Apoptosis Contributes to Cold Exposure-induced Bone Loss

Ejemplares similares