Energy starved Candidatus Pelagibacter ubique substitutes light-mediated ATP production for endogenous carbon respiration.

Previous studies have demonstrated that Candidatus Pelagibacter ubique, a member of the SAR11 clade, constitutively expresses proteorhodopsin (PR) proteins that can function as light-dependent proton pumps. However, exposure to light did not significantly improve the growth rate or final cell densit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Laura Steindler, Michael S Schwalbach, Daniel P Smith, Francis Chan, Stephen J Giovannoni
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e833de8be40c4a4a9e1034d89c1908eb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e833de8be40c4a4a9e1034d89c1908eb
record_format	dspace
spelling	oai:doaj.org-article:e833de8be40c4a4a9e1034d89c1908eb2021-11-18T06:54:16ZEnergy starved Candidatus Pelagibacter ubique substitutes light-mediated ATP production for endogenous carbon respiration.1932-620310.1371/journal.pone.0019725https://doaj.org/article/e833de8be40c4a4a9e1034d89c1908eb2011-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21573025/?tool=EBIhttps://doaj.org/toc/1932-6203Previous studies have demonstrated that Candidatus Pelagibacter ubique, a member of the SAR11 clade, constitutively expresses proteorhodopsin (PR) proteins that can function as light-dependent proton pumps. However, exposure to light did not significantly improve the growth rate or final cell densities of SAR11 isolates in a wide range of conditions. Thus, the ecophysiological role of PR in SAR11 remained unresolved. We investigated a range of cellular properties and here show that light causes dramatic changes in physiology and gene expression in Cand. P. ubique cells that are starved for carbon, but provides little or no advantage during active growth on organic carbon substrates. During logarithmic growth there was no difference in oxygen consumption by cells in light versus dark. Energy starved cells respired endogenous carbon in the dark, becoming spheres that approached the minimum predicted size for cells, and produced abundant pili. In the light, energy starved cells maintained size, ATP content, and higher substrate transport rates, and differentially expressed nearly 10% of their genome. These findings show that PR is a vital adaptation that supports Cand. P. ubique metabolism during carbon starvation, a condition that is likely to occur in the extreme conditions of ocean environments.Laura SteindlerMichael S SchwalbachDaniel P SmithFrancis ChanStephen J GiovannoniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e19725 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura Steindler Michael S Schwalbach Daniel P Smith Francis Chan Stephen J Giovannoni Energy starved Candidatus Pelagibacter ubique substitutes light-mediated ATP production for endogenous carbon respiration.
description	Previous studies have demonstrated that Candidatus Pelagibacter ubique, a member of the SAR11 clade, constitutively expresses proteorhodopsin (PR) proteins that can function as light-dependent proton pumps. However, exposure to light did not significantly improve the growth rate or final cell densities of SAR11 isolates in a wide range of conditions. Thus, the ecophysiological role of PR in SAR11 remained unresolved. We investigated a range of cellular properties and here show that light causes dramatic changes in physiology and gene expression in Cand. P. ubique cells that are starved for carbon, but provides little or no advantage during active growth on organic carbon substrates. During logarithmic growth there was no difference in oxygen consumption by cells in light versus dark. Energy starved cells respired endogenous carbon in the dark, becoming spheres that approached the minimum predicted size for cells, and produced abundant pili. In the light, energy starved cells maintained size, ATP content, and higher substrate transport rates, and differentially expressed nearly 10% of their genome. These findings show that PR is a vital adaptation that supports Cand. P. ubique metabolism during carbon starvation, a condition that is likely to occur in the extreme conditions of ocean environments.
format	article
author	Laura Steindler Michael S Schwalbach Daniel P Smith Francis Chan Stephen J Giovannoni
author_facet	Laura Steindler Michael S Schwalbach Daniel P Smith Francis Chan Stephen J Giovannoni
author_sort	Laura Steindler
title	Energy starved Candidatus Pelagibacter ubique substitutes light-mediated ATP production for endogenous carbon respiration.
title_short	Energy starved Candidatus Pelagibacter ubique substitutes light-mediated ATP production for endogenous carbon respiration.
title_full	Energy starved Candidatus Pelagibacter ubique substitutes light-mediated ATP production for endogenous carbon respiration.
title_fullStr	Energy starved Candidatus Pelagibacter ubique substitutes light-mediated ATP production for endogenous carbon respiration.
title_full_unstemmed	Energy starved Candidatus Pelagibacter ubique substitutes light-mediated ATP production for endogenous carbon respiration.
title_sort	energy starved candidatus pelagibacter ubique substitutes light-mediated atp production for endogenous carbon respiration.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/e833de8be40c4a4a9e1034d89c1908eb
work_keys_str_mv	AT laurasteindler energystarvedcandidatuspelagibacterubiquesubstituteslightmediatedatpproductionforendogenouscarbonrespiration AT michaelsschwalbach energystarvedcandidatuspelagibacterubiquesubstituteslightmediatedatpproductionforendogenouscarbonrespiration AT danielpsmith energystarvedcandidatuspelagibacterubiquesubstituteslightmediatedatpproductionforendogenouscarbonrespiration AT francischan energystarvedcandidatuspelagibacterubiquesubstituteslightmediatedatpproductionforendogenouscarbonrespiration AT stephenjgiovannoni energystarvedcandidatuspelagibacterubiquesubstituteslightmediatedatpproductionforendogenouscarbonrespiration
_version_	1718424263038337024

Energy starved Candidatus Pelagibacter ubique substitutes light-mediated ATP production for endogenous carbon respiration.

Ejemplares similares