A Pipeline for Faecal Host DNA Analysis by Absolute Quantification of LINE-1 and Mitochondrial Genomic Elements Using ddPCR

Abstract Stool contains DNA shed from cells of the gastrointestinal (GI) tract and has great potential as a bio-specimen for non-invasive, nucleic acid-based detection of GI diseases. Whereas methods for studying faecal microbiome DNA are plentiful, there is a lack of well-characterised procedures f...

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Bibliographic Details
Main Authors: Kuang He, Hideaki Fujiwara, Cynthia Zajac, Erin Sandford, Pavan Reddy, Sung Won Choi, Muneesh Tewari
Format: article
Language:EN
Published: Nature Portfolio 2019
Subjects:
R
Q
Online Access:https://doaj.org/article/e849b650cde247b3b487f89a41ef0c9a
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