Characterization of voltage-gated potassium channels in human neural progenitor cells.

<h4>Background</h4>Voltage-gated potassium (K(v)) channels are among the earliest ion channels to appear during brain development, suggesting a functional requirement for progenitor cell proliferation and/or differentiation. We tested this hypothesis, using human neural progenitor cells...

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Autores principales:	Grit Schaarschmidt, Florian Wegner, Sigrid C Schwarz, Hartmut Schmidt, Johannes Schwarz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e84f2eb897834a0cb28c411e63f40f86
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spelling	oai:doaj.org-article:e84f2eb897834a0cb28c411e63f40f862021-11-25T06:21:43ZCharacterization of voltage-gated potassium channels in human neural progenitor cells.1932-620310.1371/journal.pone.0006168https://doaj.org/article/e84f2eb897834a0cb28c411e63f40f862009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19584922/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Voltage-gated potassium (K(v)) channels are among the earliest ion channels to appear during brain development, suggesting a functional requirement for progenitor cell proliferation and/or differentiation. We tested this hypothesis, using human neural progenitor cells (hNPCs) as a model system.<h4>Methodology/principal findings</h4>In proliferating hNPCs a broad spectrum of K(v) channel subtypes was identified using quantitative real-time PCR with a predominant expression of the A-type channel K(v)4.2. In whole-cell patch-clamp recordings K(v) currents were separated into a large transient component characteristic for fast-inactivating A-type potassium channels (I(A)) and a small, sustained component produced by delayed-rectifying channels (I(K)). During differentiation the expression of I(A) as well as A-type channel transcripts dramatically decreased, while I(K) producing delayed-rectifiers were upregulated. Both K(v) currents were differentially inhibited by selective neurotoxins like phrixotoxin-1 and alpha-dendrotoxin as well as by antagonists like 4-aminopyridine, ammoniumchloride, tetraethylammonium chloride and quinidine. In viability and proliferation assays chronic inhibition of the A-type currents severely disturbed the cell cycle and precluded proper hNPC proliferation, while the blockade of delayed-rectifiers by alpha-dendrotoxin increased proliferation.<h4>Conclusions/significance</h4>These findings suggest that A-type potassium currents are essential for proper proliferation of immature multipotent hNPCs.Grit SchaarschmidtFlorian WegnerSigrid C SchwarzHartmut SchmidtJohannes SchwarzPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 7, p e6168 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Grit Schaarschmidt Florian Wegner Sigrid C Schwarz Hartmut Schmidt Johannes Schwarz Characterization of voltage-gated potassium channels in human neural progenitor cells.
description	<h4>Background</h4>Voltage-gated potassium (K(v)) channels are among the earliest ion channels to appear during brain development, suggesting a functional requirement for progenitor cell proliferation and/or differentiation. We tested this hypothesis, using human neural progenitor cells (hNPCs) as a model system.<h4>Methodology/principal findings</h4>In proliferating hNPCs a broad spectrum of K(v) channel subtypes was identified using quantitative real-time PCR with a predominant expression of the A-type channel K(v)4.2. In whole-cell patch-clamp recordings K(v) currents were separated into a large transient component characteristic for fast-inactivating A-type potassium channels (I(A)) and a small, sustained component produced by delayed-rectifying channels (I(K)). During differentiation the expression of I(A) as well as A-type channel transcripts dramatically decreased, while I(K) producing delayed-rectifiers were upregulated. Both K(v) currents were differentially inhibited by selective neurotoxins like phrixotoxin-1 and alpha-dendrotoxin as well as by antagonists like 4-aminopyridine, ammoniumchloride, tetraethylammonium chloride and quinidine. In viability and proliferation assays chronic inhibition of the A-type currents severely disturbed the cell cycle and precluded proper hNPC proliferation, while the blockade of delayed-rectifiers by alpha-dendrotoxin increased proliferation.<h4>Conclusions/significance</h4>These findings suggest that A-type potassium currents are essential for proper proliferation of immature multipotent hNPCs.
format	article
author	Grit Schaarschmidt Florian Wegner Sigrid C Schwarz Hartmut Schmidt Johannes Schwarz
author_facet	Grit Schaarschmidt Florian Wegner Sigrid C Schwarz Hartmut Schmidt Johannes Schwarz
author_sort	Grit Schaarschmidt
title	Characterization of voltage-gated potassium channels in human neural progenitor cells.
title_short	Characterization of voltage-gated potassium channels in human neural progenitor cells.
title_full	Characterization of voltage-gated potassium channels in human neural progenitor cells.
title_fullStr	Characterization of voltage-gated potassium channels in human neural progenitor cells.
title_full_unstemmed	Characterization of voltage-gated potassium channels in human neural progenitor cells.
title_sort	characterization of voltage-gated potassium channels in human neural progenitor cells.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/e84f2eb897834a0cb28c411e63f40f86
work_keys_str_mv	AT gritschaarschmidt characterizationofvoltagegatedpotassiumchannelsinhumanneuralprogenitorcells AT florianwegner characterizationofvoltagegatedpotassiumchannelsinhumanneuralprogenitorcells AT sigridcschwarz characterizationofvoltagegatedpotassiumchannelsinhumanneuralprogenitorcells AT hartmutschmidt characterizationofvoltagegatedpotassiumchannelsinhumanneuralprogenitorcells AT johannesschwarz characterizationofvoltagegatedpotassiumchannelsinhumanneuralprogenitorcells
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Characterization of voltage-gated potassium channels in human neural progenitor cells.

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