Inhibitory effect of the Kampo medicinal formula Yokukansan on acute stress-induced defecation in rats

Yasuaki Kanada, Ayami Katayama, Hideshi Ikemoto, Kana Takahashi, Mana Tsukada, Akio Nakamura, Shogo Ishino, Tadashi Hisamitsu, Masataka Sunagawa Department of Physiology, School of Medicine, Showa University, Shinagawa-ku, Tokyo, Japan Objectives: Irritable bowel syndrome (IBS) is a functional gas...

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Autores principales:	Kanada Y, Katayama A, Ikemoto H, Takahashi K, Tsukada M, Nakamura A, Ishino S, Hisamitsu T, Sunagawa M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Yokukansan oxytocin irritable bowel syndrome acute stress corticosterone Kampo medicine Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/e852aa368b924ba7862b719fc3bb3da4
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spelling	oai:doaj.org-article:e852aa368b924ba7862b719fc3bb3da42021-12-02T07:44:14ZInhibitory effect of the Kampo medicinal formula Yokukansan on acute stress-induced defecation in rats1178-2021https://doaj.org/article/e852aa368b924ba7862b719fc3bb3da42018-04-01T00:00:00Zhttps://www.dovepress.com/inhibitory-effect-of-the-kampo-medicinal-formula-yokukansan-on-acute-s-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Yasuaki Kanada, Ayami Katayama, Hideshi Ikemoto, Kana Takahashi, Mana Tsukada, Akio Nakamura, Shogo Ishino, Tadashi Hisamitsu, Masataka Sunagawa Department of Physiology, School of Medicine, Showa University, Shinagawa-ku, Tokyo, Japan Objectives: Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder with symptoms of abnormal defecation and abdominal discomfort. Psychological factors are well known to be involved in onset and exacerbation of IBS. A few studies have reported effectiveness of traditional herbal (Kampo) medicines in IBS treatment. Yokukansan (YKS) has been shown to have anti-stress and anxiolytic effects. We investigated the effect of YKS on defecation induced by stress and involvement of oxytocin (OT), a peptide hormone produced by the hypothalamus, in order to elucidate the mechanism of YKS action. Methods and results: Male Wistar rats were divided into four groups; control, YKS (300 mg/kg PO)-treated non-stress (YKS), acute stress (Stress), and YKS (300 mg/kg PO)-treated acute stress (Stress+YKS) groups. Rats in the Stress and Stress+YKS groups were exposed to a 15-min psychological stress procedure involving novel environmental stress. Levels of plasma OT in the YKS group were significantly higher compared with those in the Control group (P < 0.05), and OT levels in the Stress+YKS group were remarkably higher than those in the other groups (P < 0.01). Next, rats were divided into four groups; Stress, Stress+YKS, Atosiban (OT receptor antagonist; 1 mg/kg IP)-treated Stress+YKS (Stress+YKS+B), and OT (0.04 mg/kg IP)-treated acute stress (Stress+OT) groups. Rats were exposed to acute stress as in the previous experiment, and defecation during the stress load was measured. Administration of YKS or OT significantly inhibited defecation; however, administration of Atosiban partially abolished the inhibitory effect of YKS. Finally, direct action of YKS on motility of isolated colon was assessed. YKS (1 mg/mL, 5 mg/mL) did not inhibit spontaneous contraction. Conclusion: These results suggested that YKS influences stress-induced defecation and that increased OT secretion may be a mechanism underlying this phenomenon. Keywords: Yokukansan, oxytocin, irritable bowel syndrome, acute stress, corticosterone, Kampo medicineKanada YKatayama AIkemoto HTakahashi KTsukada MNakamura AIshino SHisamitsu TSunagawa MDove Medical PressarticleYokukansanoxytocinirritable bowel syndromeacute stresscorticosteroneKampo medicineNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 14, Pp 937-944 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Yokukansan oxytocin irritable bowel syndrome acute stress corticosterone Kampo medicine Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	Yokukansan oxytocin irritable bowel syndrome acute stress corticosterone Kampo medicine Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Kanada Y Katayama A Ikemoto H Takahashi K Tsukada M Nakamura A Ishino S Hisamitsu T Sunagawa M Inhibitory effect of the Kampo medicinal formula Yokukansan on acute stress-induced defecation in rats
description	Yasuaki Kanada, Ayami Katayama, Hideshi Ikemoto, Kana Takahashi, Mana Tsukada, Akio Nakamura, Shogo Ishino, Tadashi Hisamitsu, Masataka Sunagawa Department of Physiology, School of Medicine, Showa University, Shinagawa-ku, Tokyo, Japan Objectives: Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder with symptoms of abnormal defecation and abdominal discomfort. Psychological factors are well known to be involved in onset and exacerbation of IBS. A few studies have reported effectiveness of traditional herbal (Kampo) medicines in IBS treatment. Yokukansan (YKS) has been shown to have anti-stress and anxiolytic effects. We investigated the effect of YKS on defecation induced by stress and involvement of oxytocin (OT), a peptide hormone produced by the hypothalamus, in order to elucidate the mechanism of YKS action. Methods and results: Male Wistar rats were divided into four groups; control, YKS (300 mg/kg PO)-treated non-stress (YKS), acute stress (Stress), and YKS (300 mg/kg PO)-treated acute stress (Stress+YKS) groups. Rats in the Stress and Stress+YKS groups were exposed to a 15-min psychological stress procedure involving novel environmental stress. Levels of plasma OT in the YKS group were significantly higher compared with those in the Control group (P < 0.05), and OT levels in the Stress+YKS group were remarkably higher than those in the other groups (P < 0.01). Next, rats were divided into four groups; Stress, Stress+YKS, Atosiban (OT receptor antagonist; 1 mg/kg IP)-treated Stress+YKS (Stress+YKS+B), and OT (0.04 mg/kg IP)-treated acute stress (Stress+OT) groups. Rats were exposed to acute stress as in the previous experiment, and defecation during the stress load was measured. Administration of YKS or OT significantly inhibited defecation; however, administration of Atosiban partially abolished the inhibitory effect of YKS. Finally, direct action of YKS on motility of isolated colon was assessed. YKS (1 mg/mL, 5 mg/mL) did not inhibit spontaneous contraction. Conclusion: These results suggested that YKS influences stress-induced defecation and that increased OT secretion may be a mechanism underlying this phenomenon. Keywords: Yokukansan, oxytocin, irritable bowel syndrome, acute stress, corticosterone, Kampo medicine
format	article
author	Kanada Y Katayama A Ikemoto H Takahashi K Tsukada M Nakamura A Ishino S Hisamitsu T Sunagawa M
author_facet	Kanada Y Katayama A Ikemoto H Takahashi K Tsukada M Nakamura A Ishino S Hisamitsu T Sunagawa M
author_sort	Kanada Y
title	Inhibitory effect of the Kampo medicinal formula Yokukansan on acute stress-induced defecation in rats
title_short	Inhibitory effect of the Kampo medicinal formula Yokukansan on acute stress-induced defecation in rats
title_full	Inhibitory effect of the Kampo medicinal formula Yokukansan on acute stress-induced defecation in rats
title_fullStr	Inhibitory effect of the Kampo medicinal formula Yokukansan on acute stress-induced defecation in rats
title_full_unstemmed	Inhibitory effect of the Kampo medicinal formula Yokukansan on acute stress-induced defecation in rats
title_sort	inhibitory effect of the kampo medicinal formula yokukansan on acute stress-induced defecation in rats
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/e852aa368b924ba7862b719fc3bb3da4
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Inhibitory effect of the Kampo medicinal formula Yokukansan on acute stress-induced defecation in rats

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