The role of Psl in the failure to eradicate Pseudomonas aeruginosa biofilms in children with cystic fibrosis

Abstract The exopolysaccharide Psl contributes to biofilm structure and antibiotic tolerance and may play a role in the failure to eradicate Pseudomonas aeruginosa from cystic fibrosis (CF) airways. The study objective was to determine whether there were any differences in Psl in P. aeruginosa isola...

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Autores principales:	Amanda J. Morris, Lindsay Jackson, Yvonne CW Yau, Courtney Reichhardt, Trevor Beaudoin, Stephanie Uwumarenogie, Kevin M. Guttman, P. Lynne Howell, Matthew R. Parsek, Lucas R. Hoffman, Dao Nguyen, Antonio DiGiandomenico, David S. Guttman, Daniel J. Wozniak, Valerie J. Waters
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/e854128294d24e40bec501038888f54b
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spelling	oai:doaj.org-article:e854128294d24e40bec501038888f54b2021-12-02T16:34:05ZThe role of Psl in the failure to eradicate Pseudomonas aeruginosa biofilms in children with cystic fibrosis10.1038/s41522-021-00234-32055-5008https://doaj.org/article/e854128294d24e40bec501038888f54b2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00234-3https://doaj.org/toc/2055-5008Abstract The exopolysaccharide Psl contributes to biofilm structure and antibiotic tolerance and may play a role in the failure to eradicate Pseudomonas aeruginosa from cystic fibrosis (CF) airways. The study objective was to determine whether there were any differences in Psl in P. aeruginosa isolates that were successfully eradicated compared to those that persisted, despite inhaled tobramycin treatment, in children with CF. Initial P. aeruginosa isolates were collected from children with CF undergoing eradication treatment, grown as biofilms and labeled with 3 anti-Psl monoclonal antibodies (Cam003/Psl0096, WapR001, WapR016) before confocal microscopy visualization. When grown as biofilms, P. aeruginosa isolates from children who failed antibiotic eradication therapy, had significantly increased Psl0096 binding compared to isolates from those who cleared P. aeruginosa. This was confirmed in P. aeruginosa isolates from the SickKids Eradication Cohort as well as the Early Pseudomonas Infection Control (EPIC) trial. Increased anti-Psl antibody binding was associated with bacterial aggregation and tobramycin tolerance. The biofilm matrix represents a potential therapeutic target to improve P. aeruginosa eradication treatment.Amanda J. MorrisLindsay JacksonYvonne CW YauCourtney ReichhardtTrevor BeaudoinStephanie UwumarenogieKevin M. GuttmanP. Lynne HowellMatthew R. ParsekLucas R. HoffmanDao NguyenAntonio DiGiandomenicoDavid S. GuttmanDaniel J. WozniakValerie J. WatersNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Amanda J. Morris Lindsay Jackson Yvonne CW Yau Courtney Reichhardt Trevor Beaudoin Stephanie Uwumarenogie Kevin M. Guttman P. Lynne Howell Matthew R. Parsek Lucas R. Hoffman Dao Nguyen Antonio DiGiandomenico David S. Guttman Daniel J. Wozniak Valerie J. Waters The role of Psl in the failure to eradicate Pseudomonas aeruginosa biofilms in children with cystic fibrosis
description	Abstract The exopolysaccharide Psl contributes to biofilm structure and antibiotic tolerance and may play a role in the failure to eradicate Pseudomonas aeruginosa from cystic fibrosis (CF) airways. The study objective was to determine whether there were any differences in Psl in P. aeruginosa isolates that were successfully eradicated compared to those that persisted, despite inhaled tobramycin treatment, in children with CF. Initial P. aeruginosa isolates were collected from children with CF undergoing eradication treatment, grown as biofilms and labeled with 3 anti-Psl monoclonal antibodies (Cam003/Psl0096, WapR001, WapR016) before confocal microscopy visualization. When grown as biofilms, P. aeruginosa isolates from children who failed antibiotic eradication therapy, had significantly increased Psl0096 binding compared to isolates from those who cleared P. aeruginosa. This was confirmed in P. aeruginosa isolates from the SickKids Eradication Cohort as well as the Early Pseudomonas Infection Control (EPIC) trial. Increased anti-Psl antibody binding was associated with bacterial aggregation and tobramycin tolerance. The biofilm matrix represents a potential therapeutic target to improve P. aeruginosa eradication treatment.
format	article
author	Amanda J. Morris Lindsay Jackson Yvonne CW Yau Courtney Reichhardt Trevor Beaudoin Stephanie Uwumarenogie Kevin M. Guttman P. Lynne Howell Matthew R. Parsek Lucas R. Hoffman Dao Nguyen Antonio DiGiandomenico David S. Guttman Daniel J. Wozniak Valerie J. Waters
author_facet	Amanda J. Morris Lindsay Jackson Yvonne CW Yau Courtney Reichhardt Trevor Beaudoin Stephanie Uwumarenogie Kevin M. Guttman P. Lynne Howell Matthew R. Parsek Lucas R. Hoffman Dao Nguyen Antonio DiGiandomenico David S. Guttman Daniel J. Wozniak Valerie J. Waters
author_sort	Amanda J. Morris
title	The role of Psl in the failure to eradicate Pseudomonas aeruginosa biofilms in children with cystic fibrosis
title_short	The role of Psl in the failure to eradicate Pseudomonas aeruginosa biofilms in children with cystic fibrosis
title_full	The role of Psl in the failure to eradicate Pseudomonas aeruginosa biofilms in children with cystic fibrosis
title_fullStr	The role of Psl in the failure to eradicate Pseudomonas aeruginosa biofilms in children with cystic fibrosis
title_full_unstemmed	The role of Psl in the failure to eradicate Pseudomonas aeruginosa biofilms in children with cystic fibrosis
title_sort	role of psl in the failure to eradicate pseudomonas aeruginosa biofilms in children with cystic fibrosis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e854128294d24e40bec501038888f54b
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The role of Psl in the failure to eradicate Pseudomonas aeruginosa biofilms in children with cystic fibrosis

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