Delay in primordial germ cell migration in adamts9 knockout zebrafish

Abstract Adamts9 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 9) is one of a few metalloproteinases structurally conserved from C. elegans to humans and is indispensable in germ cell migration in invertebrates. However, adamts9′s roles in germ cell migration in ver...

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Autores principales:	Jonathan J. Carver, Yuanfa He, Yong Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e85b6597d0a7475488089932b37712bb
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spelling	oai:doaj.org-article:e85b6597d0a7475488089932b37712bb2021-12-02T16:45:14ZDelay in primordial germ cell migration in adamts9 knockout zebrafish10.1038/s41598-021-88024-x2045-2322https://doaj.org/article/e85b6597d0a7475488089932b37712bb2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88024-xhttps://doaj.org/toc/2045-2322Abstract Adamts9 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 9) is one of a few metalloproteinases structurally conserved from C. elegans to humans and is indispensable in germ cell migration in invertebrates. However, adamts9′s roles in germ cell migration in vertebrates has not been examined. In the present study, we found zygotic expression of adamts9 started around the germ ring stage and reached peak levels at 3 days post fertilization (dpf) in zebrafish. The migration of primordial germ cells (PGC) was completed within 24 hours (h) in wildtype siblings, while a delay in PGC migration was found at 15 and 24-h post-fertilization (hpf) in the Adamts9 knockout (KO). However, the delayed PGC migration in Adamts9 KO disappeared at 48 hpf. Our study suggests a conserved function of Adamts9 in germ cell migration among invertebrates and vertebrates. In addition, our results also suggest that Adamts9 is not essential for germ cell migration as reported in C. elegans, possibly due to expansion of Adamts family members and compensatory roles from other metalloproteinases in vertebrates. Further studies are required in order to elucidate the functions and mechanisms of metalloproteinases in germ cell migration and gonad formation in vertebrates.Jonathan J. CarverYuanfa HeYong ZhuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jonathan J. Carver Yuanfa He Yong Zhu Delay in primordial germ cell migration in adamts9 knockout zebrafish
description	Abstract Adamts9 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 9) is one of a few metalloproteinases structurally conserved from C. elegans to humans and is indispensable in germ cell migration in invertebrates. However, adamts9′s roles in germ cell migration in vertebrates has not been examined. In the present study, we found zygotic expression of adamts9 started around the germ ring stage and reached peak levels at 3 days post fertilization (dpf) in zebrafish. The migration of primordial germ cells (PGC) was completed within 24 hours (h) in wildtype siblings, while a delay in PGC migration was found at 15 and 24-h post-fertilization (hpf) in the Adamts9 knockout (KO). However, the delayed PGC migration in Adamts9 KO disappeared at 48 hpf. Our study suggests a conserved function of Adamts9 in germ cell migration among invertebrates and vertebrates. In addition, our results also suggest that Adamts9 is not essential for germ cell migration as reported in C. elegans, possibly due to expansion of Adamts family members and compensatory roles from other metalloproteinases in vertebrates. Further studies are required in order to elucidate the functions and mechanisms of metalloproteinases in germ cell migration and gonad formation in vertebrates.
format	article
author	Jonathan J. Carver Yuanfa He Yong Zhu
author_facet	Jonathan J. Carver Yuanfa He Yong Zhu
author_sort	Jonathan J. Carver
title	Delay in primordial germ cell migration in adamts9 knockout zebrafish
title_short	Delay in primordial germ cell migration in adamts9 knockout zebrafish
title_full	Delay in primordial germ cell migration in adamts9 knockout zebrafish
title_fullStr	Delay in primordial germ cell migration in adamts9 knockout zebrafish
title_full_unstemmed	Delay in primordial germ cell migration in adamts9 knockout zebrafish
title_sort	delay in primordial germ cell migration in adamts9 knockout zebrafish
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e85b6597d0a7475488089932b37712bb
work_keys_str_mv	AT jonathanjcarver delayinprimordialgermcellmigrationinadamts9knockoutzebrafish AT yuanfahe delayinprimordialgermcellmigrationinadamts9knockoutzebrafish AT yongzhu delayinprimordialgermcellmigrationinadamts9knockoutzebrafish
_version_	1718383488854392832

Delay in primordial germ cell migration in adamts9 knockout zebrafish

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