Antioxidant and Antimicrobial Evaluation and Chemical Investigation of Rosa gallica var. aegyptiaca Leaf Extracts

Rosa gallica var. aegyptiaca is a species of flowering plant belonging to the Rosaceae family that plays an important role as a therapeutic agent for the treatment of specific types of cancer, microbial infections, and diabetes mellitus. This work presents the f...

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Autores principales:	Ahmed S. Abdelbaky, Abir M. H. A. Mohamed, Salman S. Alharthi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>R. gallica</i> var. <i>aegyptiaca</i> antioxidant activity antimicrobial activity plant extracts HPLC-DAD total phenolic content Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/e85e86d5c7ed47abab6e4697d1ca6fd6
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spelling	oai:doaj.org-article:e85e86d5c7ed47abab6e4697d1ca6fd62021-11-11T18:29:56ZAntioxidant and Antimicrobial Evaluation and Chemical Investigation of <i>Rosa gallica</i> var. <i>aegyptiaca</i> Leaf Extracts10.3390/molecules262164981420-3049https://doaj.org/article/e85e86d5c7ed47abab6e4697d1ca6fd62021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6498https://doaj.org/toc/1420-3049<i>Rosa gallica</i> var. <i>aegyptiaca</i> is a species of flowering plant belonging to the Rosaceae family that plays an important role as a therapeutic agent for the treatment of specific types of cancer, microbial infections, and diabetes mellitus. This work presents the first report on the evaluation of the antioxidant and antimicrobial potential along with the phytochemical analysis of <i>Rosa gallica</i> var. <i>aegyptiaca</i> leaves. Five leaf extracts of hexane, chloroform, methanol, hydromethanol 80%, and water were prepared. Assessment of antioxidant activity was carried out via DPPH radical scavenging assay. Antimicrobial activity against five foodborne pathogenic bacteria—including <i>Listeria monocytogenes</i>, <i>Bacillus subtilis</i>, <i>Staphylococcus aureus</i>, <i>Escherichia coli</i>, and <i>Salmonella enteritidis</i>—and the fungus <i>Candida albicans</i>, was examined using the disc diffusion method. Total phenolic content and total flavonoid content were determined using the Folin–Ciocalteu reagent and aluminum chloride methods, respectively. Isolation, identification, and quantification of phenolic compounds were performed using HPLC-DAD analysis. Amongst the five leaf extracts that were investigated, hydromethanol 80% extract possessed the highest extraction yield, antioxidant activity, total phenolic content, and antimicrobial activity against all tested microbial strains. Moreover, this extract furnished six active phenolic compounds: gallic acid (1), (+) catechin (2), chlorogenic acid (3), (–) epicatechin (4), quercetin-3-<i>O</i>-α-<span style="font-variant: small-caps;">d</span>-(glucopyranoside) (5), and quercetin (6). This study provides an alternative utilization of <i>R. gallica</i> var. <i>aegyptiaca</i> leaves as a readily accessible source of natural antioxidants and antimicrobials in the food and pharmaceutical industries.Ahmed S. AbdelbakyAbir M. H. A. MohamedSalman S. AlharthiMDPI AGarticle<i>R. gallica</i> var. <i>aegyptiaca</i>antioxidant activityantimicrobial activityplant extractsHPLC-DADtotal phenolic contentOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6498, p 6498 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>R. gallica</i> var. <i>aegyptiaca</i> antioxidant activity antimicrobial activity plant extracts HPLC-DAD total phenolic content Organic chemistry QD241-441
spellingShingle	<i>R. gallica</i> var. <i>aegyptiaca</i> antioxidant activity antimicrobial activity plant extracts HPLC-DAD total phenolic content Organic chemistry QD241-441 Ahmed S. Abdelbaky Abir M. H. A. Mohamed Salman S. Alharthi Antioxidant and Antimicrobial Evaluation and Chemical Investigation of <i>Rosa gallica</i> var. <i>aegyptiaca</i> Leaf Extracts
description	<i>Rosa gallica</i> var. <i>aegyptiaca</i> is a species of flowering plant belonging to the Rosaceae family that plays an important role as a therapeutic agent for the treatment of specific types of cancer, microbial infections, and diabetes mellitus. This work presents the first report on the evaluation of the antioxidant and antimicrobial potential along with the phytochemical analysis of <i>Rosa gallica</i> var. <i>aegyptiaca</i> leaves. Five leaf extracts of hexane, chloroform, methanol, hydromethanol 80%, and water were prepared. Assessment of antioxidant activity was carried out via DPPH radical scavenging assay. Antimicrobial activity against five foodborne pathogenic bacteria—including <i>Listeria monocytogenes</i>, <i>Bacillus subtilis</i>, <i>Staphylococcus aureus</i>, <i>Escherichia coli</i>, and <i>Salmonella enteritidis</i>—and the fungus <i>Candida albicans</i>, was examined using the disc diffusion method. Total phenolic content and total flavonoid content were determined using the Folin–Ciocalteu reagent and aluminum chloride methods, respectively. Isolation, identification, and quantification of phenolic compounds were performed using HPLC-DAD analysis. Amongst the five leaf extracts that were investigated, hydromethanol 80% extract possessed the highest extraction yield, antioxidant activity, total phenolic content, and antimicrobial activity against all tested microbial strains. Moreover, this extract furnished six active phenolic compounds: gallic acid (1), (+) catechin (2), chlorogenic acid (3), (–) epicatechin (4), quercetin-3-<i>O</i>-α-<span style="font-variant: small-caps;">d</span>-(glucopyranoside) (5), and quercetin (6). This study provides an alternative utilization of <i>R. gallica</i> var. <i>aegyptiaca</i> leaves as a readily accessible source of natural antioxidants and antimicrobials in the food and pharmaceutical industries.
format	article
author	Ahmed S. Abdelbaky Abir M. H. A. Mohamed Salman S. Alharthi
author_facet	Ahmed S. Abdelbaky Abir M. H. A. Mohamed Salman S. Alharthi
author_sort	Ahmed S. Abdelbaky
title	Antioxidant and Antimicrobial Evaluation and Chemical Investigation of <i>Rosa gallica</i> var. <i>aegyptiaca</i> Leaf Extracts
title_short	Antioxidant and Antimicrobial Evaluation and Chemical Investigation of <i>Rosa gallica</i> var. <i>aegyptiaca</i> Leaf Extracts
title_full	Antioxidant and Antimicrobial Evaluation and Chemical Investigation of <i>Rosa gallica</i> var. <i>aegyptiaca</i> Leaf Extracts
title_fullStr	Antioxidant and Antimicrobial Evaluation and Chemical Investigation of <i>Rosa gallica</i> var. <i>aegyptiaca</i> Leaf Extracts
title_full_unstemmed	Antioxidant and Antimicrobial Evaluation and Chemical Investigation of <i>Rosa gallica</i> var. <i>aegyptiaca</i> Leaf Extracts
title_sort	antioxidant and antimicrobial evaluation and chemical investigation of <i>rosa gallica</i> var. <i>aegyptiaca</i> leaf extracts
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e85e86d5c7ed47abab6e4697d1ca6fd6
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Antioxidant and Antimicrobial Evaluation and Chemical Investigation of <i>Rosa gallica</i> var. <i>aegyptiaca</i> Leaf Extracts

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