<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Alters Its Transcriptome Related to Carbon Metabolism and Virulence as a Possible Survival Strategy in Blood from Trauma Patients

ABSTRACT Trauma patients (TPs) are highly susceptible to infections, which often lead to sepsis. Among the numerous causative agents, Pseudomonas aeruginosa is especially important, as P. aeruginosa sepsis is often fatal. Understanding the mechanism of its pathogenesis in bloodstream infections is i...

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Autores principales:	Moamen M. Elmassry, Nithya S. Mudaliar, Kameswara Rao Kottapalli, Sharmila Dissanaike, John A. Griswold, Michael J. San Francisco, Jane A. Colmer-Hamood, Abdul N. Hamood
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Pseudomonas aeruginosa blood metabolism metabolome sepsis transcriptome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e85eca1d79ba45fca64363b73d372732
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spelling	oai:doaj.org-article:e85eca1d79ba45fca64363b73d3727322021-12-02T18:44:37Z<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Alters Its Transcriptome Related to Carbon Metabolism and Virulence as a Possible Survival Strategy in Blood from Trauma Patients10.1128/mSystems.00312-182379-5077https://doaj.org/article/e85eca1d79ba45fca64363b73d3727322019-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00312-18https://doaj.org/toc/2379-5077ABSTRACT Trauma patients (TPs) are highly susceptible to infections, which often lead to sepsis. Among the numerous causative agents, Pseudomonas aeruginosa is especially important, as P. aeruginosa sepsis is often fatal. Understanding the mechanism of its pathogenesis in bloodstream infections is imperative; however, this mechanism has not been previously described. To examine the effect of trauma-induced changes in blood on the expression of P. aeruginosa genes, we grew strain UCBPP-PA14 (PA14) in blood samples from eight TPs and seven healthy volunteers (HVs). Compared with its growth in blood from HVs, the growth of PA14 in blood from TPs significantly altered the expression of 285 genes. Genes whose expression was significantly increased were related to carbon metabolism, especially malonate utilization and mannitol uptake, and efflux of heavy metals. Genes whose expression was significantly reduced included genes of the type VI secretion system, genes related to uptake and metabolism of amino acids, and genes related to biosynthesis and transport of the siderophores pyoverdine and pyochelin. These results suggest that during systemic infection in trauma patients, and to adapt to the trauma-induced changes in blood, P. aeruginosa adjusts positively and negatively the expression of numerous genes related to carbon metabolism and virulence, respectively. IMPORTANCE While a considerable body of knowledge regarding sepsis in trauma patients is available, the potential influence of trauma-induced changes in the blood of these patients on the pathogenesis of Pseudomonas aeruginosa is basically an unexplored area. Rather than using standard laboratory media, we grew P. aeruginosa in whole blood from either healthy volunteers or trauma patients. The specific changes in the P. aeruginosa transcriptome in response to growth in blood from trauma patients reflect the adaptation of this organism to the bloodstream environment. This knowledge is vital for understanding the strategies this pathogen uses to adapt and survive within the host during systemic infection. Such information will help researchers and clinicians to develop new approaches for treatment of sepsis caused by P. aeruginosa in trauma patients, especially in terms of recognizing the effects of specific therapies (e.g., iron, zinc, or mannitol) on the organism. Further, this information can most likely be extrapolated to all patients with P. aeruginosa septicemia. Author Video: An author video summary of this article is available.Moamen M. ElmassryNithya S. MudaliarKameswara Rao KottapalliSharmila DissanaikeJohn A. GriswoldMichael J. San FranciscoJane A. Colmer-HamoodAbdul N. HamoodAmerican Society for MicrobiologyarticlePseudomonas aeruginosabloodmetabolismmetabolomesepsistranscriptomeMicrobiologyQR1-502ENmSystems, Vol 4, Iss 4 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pseudomonas aeruginosa blood metabolism metabolome sepsis transcriptome Microbiology QR1-502
spellingShingle	Pseudomonas aeruginosa blood metabolism metabolome sepsis transcriptome Microbiology QR1-502 Moamen M. Elmassry Nithya S. Mudaliar Kameswara Rao Kottapalli Sharmila Dissanaike John A. Griswold Michael J. San Francisco Jane A. Colmer-Hamood Abdul N. Hamood <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Alters Its Transcriptome Related to Carbon Metabolism and Virulence as a Possible Survival Strategy in Blood from Trauma Patients
description	ABSTRACT Trauma patients (TPs) are highly susceptible to infections, which often lead to sepsis. Among the numerous causative agents, Pseudomonas aeruginosa is especially important, as P. aeruginosa sepsis is often fatal. Understanding the mechanism of its pathogenesis in bloodstream infections is imperative; however, this mechanism has not been previously described. To examine the effect of trauma-induced changes in blood on the expression of P. aeruginosa genes, we grew strain UCBPP-PA14 (PA14) in blood samples from eight TPs and seven healthy volunteers (HVs). Compared with its growth in blood from HVs, the growth of PA14 in blood from TPs significantly altered the expression of 285 genes. Genes whose expression was significantly increased were related to carbon metabolism, especially malonate utilization and mannitol uptake, and efflux of heavy metals. Genes whose expression was significantly reduced included genes of the type VI secretion system, genes related to uptake and metabolism of amino acids, and genes related to biosynthesis and transport of the siderophores pyoverdine and pyochelin. These results suggest that during systemic infection in trauma patients, and to adapt to the trauma-induced changes in blood, P. aeruginosa adjusts positively and negatively the expression of numerous genes related to carbon metabolism and virulence, respectively. IMPORTANCE While a considerable body of knowledge regarding sepsis in trauma patients is available, the potential influence of trauma-induced changes in the blood of these patients on the pathogenesis of Pseudomonas aeruginosa is basically an unexplored area. Rather than using standard laboratory media, we grew P. aeruginosa in whole blood from either healthy volunteers or trauma patients. The specific changes in the P. aeruginosa transcriptome in response to growth in blood from trauma patients reflect the adaptation of this organism to the bloodstream environment. This knowledge is vital for understanding the strategies this pathogen uses to adapt and survive within the host during systemic infection. Such information will help researchers and clinicians to develop new approaches for treatment of sepsis caused by P. aeruginosa in trauma patients, especially in terms of recognizing the effects of specific therapies (e.g., iron, zinc, or mannitol) on the organism. Further, this information can most likely be extrapolated to all patients with P. aeruginosa septicemia. Author Video: An author video summary of this article is available.
format	article
author	Moamen M. Elmassry Nithya S. Mudaliar Kameswara Rao Kottapalli Sharmila Dissanaike John A. Griswold Michael J. San Francisco Jane A. Colmer-Hamood Abdul N. Hamood
author_facet	Moamen M. Elmassry Nithya S. Mudaliar Kameswara Rao Kottapalli Sharmila Dissanaike John A. Griswold Michael J. San Francisco Jane A. Colmer-Hamood Abdul N. Hamood
author_sort	Moamen M. Elmassry
title	<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Alters Its Transcriptome Related to Carbon Metabolism and Virulence as a Possible Survival Strategy in Blood from Trauma Patients
title_short	<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Alters Its Transcriptome Related to Carbon Metabolism and Virulence as a Possible Survival Strategy in Blood from Trauma Patients
title_full	<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Alters Its Transcriptome Related to Carbon Metabolism and Virulence as a Possible Survival Strategy in Blood from Trauma Patients
title_fullStr	<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Alters Its Transcriptome Related to Carbon Metabolism and Virulence as a Possible Survival Strategy in Blood from Trauma Patients
title_full_unstemmed	<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Alters Its Transcriptome Related to Carbon Metabolism and Virulence as a Possible Survival Strategy in Blood from Trauma Patients
title_sort	<named-content content-type="genus-species">pseudomonas aeruginosa</named-content> alters its transcriptome related to carbon metabolism and virulence as a possible survival strategy in blood from trauma patients
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/e85eca1d79ba45fca64363b73d372732
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<named-content content-type="genus-species">Pseudomonas aeruginosa</named-content> Alters Its Transcriptome Related to Carbon Metabolism and Virulence as a Possible Survival Strategy in Blood from Trauma Patients

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