Nociception and conditioned fear in rats: strains matter.

When using rats in pain research, strain-related differences in outcomes of tests for pain and nociception are acknowledged. However, very little is known about the specific characteristics of these strain differences. In this study four phylogenetically distant inbred rat strains, i.e. Wistar Kyoto...

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Autores principales:	Manon W H Schaap, Hugo van Oostrom, Arie Doornenbal, José van 't Klooster, Annemarie M Baars, Saskia S Arndt, Ludo J Hellebrekers
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e8645e55c7a64085a2160e09fb367d82
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spelling	oai:doaj.org-article:e8645e55c7a64085a2160e09fb367d822021-11-18T08:40:37ZNociception and conditioned fear in rats: strains matter.1932-620310.1371/journal.pone.0083339https://doaj.org/article/e8645e55c7a64085a2160e09fb367d822013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24376690/?tool=EBIhttps://doaj.org/toc/1932-6203When using rats in pain research, strain-related differences in outcomes of tests for pain and nociception are acknowledged. However, very little is known about the specific characteristics of these strain differences. In this study four phylogenetically distant inbred rat strains, i.e. Wistar Kyoto (WKY), Fawn Hooded (FH), Brown Norway (BN) and Lewis (LE), were investigated in different tests related to pain and nociception. During Pavlovian fear conditioning, the LE and WKY showed a significantly longer duration of freezing behaviour than the FH and BN. Additionally, differences in c-Fos expression in subregions of the prefrontal cortex and amygdala between rat strains during retrieval and expression of conditioned fear were found. For example, the BN did not show recruitment of the basolateral amygdala, whereas the WKY, FH and LE did. During the hot plate test, the WKY and LE showed a lower thermal threshold compared to the BN and FH. In a follow-up experiment, the two most contrasting strains regarding behaviour during the hot plate test and Pavlovian fear conditioning (i.e. FH and WKY) were selected and the hot plate test, Von Frey test and somatosensory-evoked potential (SEP) were investigated. During the Von Frey test, the WKY showed a lower mechanical threshold compared to the FH. When measuring the SEP, the FH appeared to be less reactive to increasing stimulus intensities when considering both peak amplitudes and latencies. Altogether, the combined results indicate various differences between rat strains in Pavlovian fear conditioning, nociception related behaviours and nociceptive processing. These findings demonstrate the necessity of using multiple rat strains when using tests including noxious stimuli and suggest that the choice of rat strains should be considered. When selecting a strain for a particular study it should be considered how this strain behaves during the tests used in that study.Manon W H SchaapHugo van OostromArie DoornenbalJosé van 't KloosterAnnemarie M BaarsSaskia S ArndtLudo J HellebrekersPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e83339 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manon W H Schaap Hugo van Oostrom Arie Doornenbal José van 't Klooster Annemarie M Baars Saskia S Arndt Ludo J Hellebrekers Nociception and conditioned fear in rats: strains matter.
description	When using rats in pain research, strain-related differences in outcomes of tests for pain and nociception are acknowledged. However, very little is known about the specific characteristics of these strain differences. In this study four phylogenetically distant inbred rat strains, i.e. Wistar Kyoto (WKY), Fawn Hooded (FH), Brown Norway (BN) and Lewis (LE), were investigated in different tests related to pain and nociception. During Pavlovian fear conditioning, the LE and WKY showed a significantly longer duration of freezing behaviour than the FH and BN. Additionally, differences in c-Fos expression in subregions of the prefrontal cortex and amygdala between rat strains during retrieval and expression of conditioned fear were found. For example, the BN did not show recruitment of the basolateral amygdala, whereas the WKY, FH and LE did. During the hot plate test, the WKY and LE showed a lower thermal threshold compared to the BN and FH. In a follow-up experiment, the two most contrasting strains regarding behaviour during the hot plate test and Pavlovian fear conditioning (i.e. FH and WKY) were selected and the hot plate test, Von Frey test and somatosensory-evoked potential (SEP) were investigated. During the Von Frey test, the WKY showed a lower mechanical threshold compared to the FH. When measuring the SEP, the FH appeared to be less reactive to increasing stimulus intensities when considering both peak amplitudes and latencies. Altogether, the combined results indicate various differences between rat strains in Pavlovian fear conditioning, nociception related behaviours and nociceptive processing. These findings demonstrate the necessity of using multiple rat strains when using tests including noxious stimuli and suggest that the choice of rat strains should be considered. When selecting a strain for a particular study it should be considered how this strain behaves during the tests used in that study.
format	article
author	Manon W H Schaap Hugo van Oostrom Arie Doornenbal José van 't Klooster Annemarie M Baars Saskia S Arndt Ludo J Hellebrekers
author_facet	Manon W H Schaap Hugo van Oostrom Arie Doornenbal José van 't Klooster Annemarie M Baars Saskia S Arndt Ludo J Hellebrekers
author_sort	Manon W H Schaap
title	Nociception and conditioned fear in rats: strains matter.
title_short	Nociception and conditioned fear in rats: strains matter.
title_full	Nociception and conditioned fear in rats: strains matter.
title_fullStr	Nociception and conditioned fear in rats: strains matter.
title_full_unstemmed	Nociception and conditioned fear in rats: strains matter.
title_sort	nociception and conditioned fear in rats: strains matter.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e8645e55c7a64085a2160e09fb367d82
work_keys_str_mv	AT manonwhschaap nociceptionandconditionedfearinratsstrainsmatter AT hugovanoostrom nociceptionandconditionedfearinratsstrainsmatter AT ariedoornenbal nociceptionandconditionedfearinratsstrainsmatter AT josevantklooster nociceptionandconditionedfearinratsstrainsmatter AT annemariembaars nociceptionandconditionedfearinratsstrainsmatter AT saskiasarndt nociceptionandconditionedfearinratsstrainsmatter AT ludojhellebrekers nociceptionandconditionedfearinratsstrainsmatter
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Nociception and conditioned fear in rats: strains matter.

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