The nose is the best niche for detection of experimental pneumococcal colonisation in adults of all ages, using nasal wash
Abstract Previous studies have suggested that the pneumococcal niche changes from the nasopharynx to the oral cavity with age. We use an Experimental Human Pneumococcal Challenge model to investigate pneumococcal colonisation in different anatomical niches with age. Healthy adults (n = 112) were int...
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Nature Portfolio
2021
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oai:doaj.org-article:e86701c2f70341c0a89b2827ecb7542b2021-12-02T15:33:23ZThe nose is the best niche for detection of experimental pneumococcal colonisation in adults of all ages, using nasal wash10.1038/s41598-021-97807-12045-2322https://doaj.org/article/e86701c2f70341c0a89b2827ecb7542b2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97807-1https://doaj.org/toc/2045-2322Abstract Previous studies have suggested that the pneumococcal niche changes from the nasopharynx to the oral cavity with age. We use an Experimental Human Pneumococcal Challenge model to investigate pneumococcal colonisation in different anatomical niches with age. Healthy adults (n = 112) were intranasally inoculated with Streptococcus pneumoniae serotype 6B (Spn6B) and were categorised as young 18–55 years (n = 57) or older > 55 years (n = 55). Colonisation status (frequency and density) was determined by multiplex qPCR targeting the lytA and cpsA-6A/B genes in both raw and culture-enriched nasal wash and oropharyngeal swab samples collected at 2-, 7- and 14-days post-exposure. For older adults, raw and culture-enriched saliva samples were also assessed. 64% of NW samples and 54% of OPS samples were positive for Spn6B in young adults, compared to 35% of NW samples, 24% of OPS samples and 6% of saliva samples in older adults. Many colonisation events were only detected in culture-enriched samples. Experimental colonisation was detected in 72% of young adults by NW and 63% by OPS. In older adults, this was 51% by NW, 36% by OPS and 9% by saliva. The nose, as assessed by nasal wash, is the best niche for detection of experimental pneumococcal colonisation in both young and older adults.Elissavet NikolaouEsther L. GermanAnnie BlizardAshleigh HowardLisa HitchinsTao ChenJim ChadwickSherin PojarElena MitsiCarla SolórzanoSyba SunnyFelicity DunneJenna F. GritzfeldHugh AdlerJason HindsKatherine A. GouldJamie RylanceAndrea M. CollinsStephen B. GordonDaniela M. FerreiraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021) |
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Medicine R Science Q Elissavet Nikolaou Esther L. German Annie Blizard Ashleigh Howard Lisa Hitchins Tao Chen Jim Chadwick Sherin Pojar Elena Mitsi Carla Solórzano Syba Sunny Felicity Dunne Jenna F. Gritzfeld Hugh Adler Jason Hinds Katherine A. Gould Jamie Rylance Andrea M. Collins Stephen B. Gordon Daniela M. Ferreira The nose is the best niche for detection of experimental pneumococcal colonisation in adults of all ages, using nasal wash |
| description |
Abstract Previous studies have suggested that the pneumococcal niche changes from the nasopharynx to the oral cavity with age. We use an Experimental Human Pneumococcal Challenge model to investigate pneumococcal colonisation in different anatomical niches with age. Healthy adults (n = 112) were intranasally inoculated with Streptococcus pneumoniae serotype 6B (Spn6B) and were categorised as young 18–55 years (n = 57) or older > 55 years (n = 55). Colonisation status (frequency and density) was determined by multiplex qPCR targeting the lytA and cpsA-6A/B genes in both raw and culture-enriched nasal wash and oropharyngeal swab samples collected at 2-, 7- and 14-days post-exposure. For older adults, raw and culture-enriched saliva samples were also assessed. 64% of NW samples and 54% of OPS samples were positive for Spn6B in young adults, compared to 35% of NW samples, 24% of OPS samples and 6% of saliva samples in older adults. Many colonisation events were only detected in culture-enriched samples. Experimental colonisation was detected in 72% of young adults by NW and 63% by OPS. In older adults, this was 51% by NW, 36% by OPS and 9% by saliva. The nose, as assessed by nasal wash, is the best niche for detection of experimental pneumococcal colonisation in both young and older adults. |
| format |
article |
| author |
Elissavet Nikolaou Esther L. German Annie Blizard Ashleigh Howard Lisa Hitchins Tao Chen Jim Chadwick Sherin Pojar Elena Mitsi Carla Solórzano Syba Sunny Felicity Dunne Jenna F. Gritzfeld Hugh Adler Jason Hinds Katherine A. Gould Jamie Rylance Andrea M. Collins Stephen B. Gordon Daniela M. Ferreira |
| author_facet |
Elissavet Nikolaou Esther L. German Annie Blizard Ashleigh Howard Lisa Hitchins Tao Chen Jim Chadwick Sherin Pojar Elena Mitsi Carla Solórzano Syba Sunny Felicity Dunne Jenna F. Gritzfeld Hugh Adler Jason Hinds Katherine A. Gould Jamie Rylance Andrea M. Collins Stephen B. Gordon Daniela M. Ferreira |
| author_sort |
Elissavet Nikolaou |
| title |
The nose is the best niche for detection of experimental pneumococcal colonisation in adults of all ages, using nasal wash |
| title_short |
The nose is the best niche for detection of experimental pneumococcal colonisation in adults of all ages, using nasal wash |
| title_full |
The nose is the best niche for detection of experimental pneumococcal colonisation in adults of all ages, using nasal wash |
| title_fullStr |
The nose is the best niche for detection of experimental pneumococcal colonisation in adults of all ages, using nasal wash |
| title_full_unstemmed |
The nose is the best niche for detection of experimental pneumococcal colonisation in adults of all ages, using nasal wash |
| title_sort |
nose is the best niche for detection of experimental pneumococcal colonisation in adults of all ages, using nasal wash |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/e86701c2f70341c0a89b2827ecb7542b |
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