Preventive Effect of <i>M. cochinchinensis</i> on Melanogenesis via Tyrosinase Activity Inhibition and p-PKC Signaling in Melan-A Cell

Whitening research is of particular interest in the cosmetics market. The main focus of whitening research is on melanogenesis inhibition through tyrosinase activity. The mechanism of melanogenesis is involved with tyrosinase activity and p-PKC signaling. In this study, we used <i>Momordica co...

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Autores principales:	Juyong Kim, Sung-Chul Hong, Eun Ha Lee, Jae Wook Lee, Seung-Hoon Yang, Jin-Chul Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>M. cochinchinensis</i> melanogenesis tyrosinase PKC signaling melan-A Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/e869503c84ea431dbb735935418cc041
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spelling	oai:doaj.org-article:e869503c84ea431dbb735935418cc0412021-11-25T18:35:00ZPreventive Effect of <i>M. cochinchinensis</i> on Melanogenesis via Tyrosinase Activity Inhibition and p-PKC Signaling in Melan-A Cell10.3390/nu131138942072-6643https://doaj.org/article/e869503c84ea431dbb735935418cc0412021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/3894https://doaj.org/toc/2072-6643Whitening research is of particular interest in the cosmetics market. The main focus of whitening research is on melanogenesis inhibition through tyrosinase activity. The mechanism of melanogenesis is involved with tyrosinase activity and p-PKC signaling. In this study, we used <i>Momordica cochinchinensis</i> (<i>Lour.</i>) <i>spreng</i>, a tropical fruit found throughout Southeast Asia, to investigate the inhibitory effect of melanogenesis. <i>M. cochinchinensis</i> contains a high concentration of polyphenols, flavonoids, and unsaturated fatty acids, which might be related to antioxidant activity. This study aimed to determine whether <i>M. cochinchinensis</i> extracts inhibit melanin synthesis in melan-A cells by inhibiting tyrosinase activity and p-PKC signaling. <i>M. cochinchinensis</i> was divided into pulp and aril and extracted under various conditions, and it was confirmed that all pulp and aril extracts have high contents of both phenols and flavonoids. Melan-A cells were treated with PMA for three days to induce melanin synthesis. After PMA treatment, <i>M. cochinchinensis</i> extracts were added to cultured media in a dose-dependent manner. Melanin contents and MTS were used to determine the amount of melanin in live cells. <i>M. cochinchinensis</i> extracts were evaluated for their effects on tyrosinase activity and p-PKC signaling pathways by Western blotting. It was found that <i>M. cochinchinensis</i> extract treatment decreased the amount of melanin and suppressed p-PKC expression. Additionally, tyrosinase activity was reduced after <i>M. cochinchinensis</i> extract treatment in a dose-dependent manner. Therefore, it was concluded that <i>M. cochinchinensis</i> could be used in antimelanogenesis and functional cosmetic materials to improve whitening.Juyong KimSung-Chul HongEun Ha LeeJae Wook LeeSeung-Hoon YangJin-Chul KimMDPI AGarticle<i>M. cochinchinensis</i>melanogenesistyrosinasePKC signalingmelan-ANutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 3894, p 3894 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>M. cochinchinensis</i> melanogenesis tyrosinase PKC signaling melan-A Nutrition. Foods and food supply TX341-641
spellingShingle	<i>M. cochinchinensis</i> melanogenesis tyrosinase PKC signaling melan-A Nutrition. Foods and food supply TX341-641 Juyong Kim Sung-Chul Hong Eun Ha Lee Jae Wook Lee Seung-Hoon Yang Jin-Chul Kim Preventive Effect of <i>M. cochinchinensis</i> on Melanogenesis via Tyrosinase Activity Inhibition and p-PKC Signaling in Melan-A Cell
description	Whitening research is of particular interest in the cosmetics market. The main focus of whitening research is on melanogenesis inhibition through tyrosinase activity. The mechanism of melanogenesis is involved with tyrosinase activity and p-PKC signaling. In this study, we used <i>Momordica cochinchinensis</i> (<i>Lour.</i>) <i>spreng</i>, a tropical fruit found throughout Southeast Asia, to investigate the inhibitory effect of melanogenesis. <i>M. cochinchinensis</i> contains a high concentration of polyphenols, flavonoids, and unsaturated fatty acids, which might be related to antioxidant activity. This study aimed to determine whether <i>M. cochinchinensis</i> extracts inhibit melanin synthesis in melan-A cells by inhibiting tyrosinase activity and p-PKC signaling. <i>M. cochinchinensis</i> was divided into pulp and aril and extracted under various conditions, and it was confirmed that all pulp and aril extracts have high contents of both phenols and flavonoids. Melan-A cells were treated with PMA for three days to induce melanin synthesis. After PMA treatment, <i>M. cochinchinensis</i> extracts were added to cultured media in a dose-dependent manner. Melanin contents and MTS were used to determine the amount of melanin in live cells. <i>M. cochinchinensis</i> extracts were evaluated for their effects on tyrosinase activity and p-PKC signaling pathways by Western blotting. It was found that <i>M. cochinchinensis</i> extract treatment decreased the amount of melanin and suppressed p-PKC expression. Additionally, tyrosinase activity was reduced after <i>M. cochinchinensis</i> extract treatment in a dose-dependent manner. Therefore, it was concluded that <i>M. cochinchinensis</i> could be used in antimelanogenesis and functional cosmetic materials to improve whitening.
format	article
author	Juyong Kim Sung-Chul Hong Eun Ha Lee Jae Wook Lee Seung-Hoon Yang Jin-Chul Kim
author_facet	Juyong Kim Sung-Chul Hong Eun Ha Lee Jae Wook Lee Seung-Hoon Yang Jin-Chul Kim
author_sort	Juyong Kim
title	Preventive Effect of <i>M. cochinchinensis</i> on Melanogenesis via Tyrosinase Activity Inhibition and p-PKC Signaling in Melan-A Cell
title_short	Preventive Effect of <i>M. cochinchinensis</i> on Melanogenesis via Tyrosinase Activity Inhibition and p-PKC Signaling in Melan-A Cell
title_full	Preventive Effect of <i>M. cochinchinensis</i> on Melanogenesis via Tyrosinase Activity Inhibition and p-PKC Signaling in Melan-A Cell
title_fullStr	Preventive Effect of <i>M. cochinchinensis</i> on Melanogenesis via Tyrosinase Activity Inhibition and p-PKC Signaling in Melan-A Cell
title_full_unstemmed	Preventive Effect of <i>M. cochinchinensis</i> on Melanogenesis via Tyrosinase Activity Inhibition and p-PKC Signaling in Melan-A Cell
title_sort	preventive effect of <i>m. cochinchinensis</i> on melanogenesis via tyrosinase activity inhibition and p-pkc signaling in melan-a cell
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e869503c84ea431dbb735935418cc041
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Preventive Effect of <i>M. cochinchinensis</i> on Melanogenesis via Tyrosinase Activity Inhibition and p-PKC Signaling in Melan-A Cell

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