Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster

Automatic quantification of image parameters is a powerful and necessary tool to explore and analyze crucial cell biological processes. This article describes two ImageJ/Fiji automated macros to approach the analysis of synaptic autophagy and exosome release from 2D confocal images. Emerging studies...

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Autores principales:	Irene Sanchez-Mirasierra, Sergio Hernandez-Diaz, Saurav Ghimire, Carla Montecinos-Oliva, Sandra-Fausia Soukup
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	autophagy autophagosome Drosophila exosome release quantification synapse Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e86e55a4f59c4204a0d89998060b6edd
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spelling	oai:doaj.org-article:e86e55a4f59c4204a0d89998060b6edd2021-11-15T06:25:19ZMacros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster2296-634X10.3389/fcell.2021.773861https://doaj.org/article/e86e55a4f59c4204a0d89998060b6edd2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.773861/fullhttps://doaj.org/toc/2296-634XAutomatic quantification of image parameters is a powerful and necessary tool to explore and analyze crucial cell biological processes. This article describes two ImageJ/Fiji automated macros to approach the analysis of synaptic autophagy and exosome release from 2D confocal images. Emerging studies point out that exosome biogenesis and autophagy share molecular and organelle components. Indeed, the crosstalk between these two processes may be relevant for brain physiology, neuronal development, and the onset/progression of neurodegenerative disorders. In this context, we describe here the macros “Autophagoquant” and “Exoquant” to assess the quantification of autophagosomes and exosomes at the neuronal presynapse of the Neuromuscular Junction (NMJ) in Drosophila melanogaster using confocal microscopy images. The Drosophila NMJ is a valuable model for the study of synapse biology, autophagy, and exosome release. By use of Autophagoquant and Exoquant, researchers can have an unbiased, standardized, and rapid tool to analyze autophagy and exosomal release in Drosophila NMJ.Code available at: https://github.com/IreneSaMi/Exoquant-AutophagoquantIrene Sanchez-MirasierraSergio Hernandez-DiazSaurav GhimireCarla Montecinos-OlivaSandra-Fausia SoukupFrontiers Media S.A.articleautophagyautophagosomeDrosophilaexosome releasequantificationsynapseBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	autophagy autophagosome Drosophila exosome release quantification synapse Biology (General) QH301-705.5
spellingShingle	autophagy autophagosome Drosophila exosome release quantification synapse Biology (General) QH301-705.5 Irene Sanchez-Mirasierra Sergio Hernandez-Diaz Saurav Ghimire Carla Montecinos-Oliva Sandra-Fausia Soukup Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster
description	Automatic quantification of image parameters is a powerful and necessary tool to explore and analyze crucial cell biological processes. This article describes two ImageJ/Fiji automated macros to approach the analysis of synaptic autophagy and exosome release from 2D confocal images. Emerging studies point out that exosome biogenesis and autophagy share molecular and organelle components. Indeed, the crosstalk between these two processes may be relevant for brain physiology, neuronal development, and the onset/progression of neurodegenerative disorders. In this context, we describe here the macros “Autophagoquant” and “Exoquant” to assess the quantification of autophagosomes and exosomes at the neuronal presynapse of the Neuromuscular Junction (NMJ) in Drosophila melanogaster using confocal microscopy images. The Drosophila NMJ is a valuable model for the study of synapse biology, autophagy, and exosome release. By use of Autophagoquant and Exoquant, researchers can have an unbiased, standardized, and rapid tool to analyze autophagy and exosomal release in Drosophila NMJ.Code available at: https://github.com/IreneSaMi/Exoquant-Autophagoquant
format	article
author	Irene Sanchez-Mirasierra Sergio Hernandez-Diaz Saurav Ghimire Carla Montecinos-Oliva Sandra-Fausia Soukup
author_facet	Irene Sanchez-Mirasierra Sergio Hernandez-Diaz Saurav Ghimire Carla Montecinos-Oliva Sandra-Fausia Soukup
author_sort	Irene Sanchez-Mirasierra
title	Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster
title_short	Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster
title_full	Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster
title_fullStr	Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster
title_full_unstemmed	Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster
title_sort	macros to quantify exosome release and autophagy at the neuromuscular junction of drosophila melanogaster
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e86e55a4f59c4204a0d89998060b6edd
work_keys_str_mv	AT irenesanchezmirasierra macrostoquantifyexosomereleaseandautophagyattheneuromuscularjunctionofdrosophilamelanogaster AT sergiohernandezdiaz macrostoquantifyexosomereleaseandautophagyattheneuromuscularjunctionofdrosophilamelanogaster AT sauravghimire macrostoquantifyexosomereleaseandautophagyattheneuromuscularjunctionofdrosophilamelanogaster AT carlamontecinosoliva macrostoquantifyexosomereleaseandautophagyattheneuromuscularjunctionofdrosophilamelanogaster AT sandrafausiasoukup macrostoquantifyexosomereleaseandautophagyattheneuromuscularjunctionofdrosophilamelanogaster
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Macros to Quantify Exosome Release and Autophagy at the Neuromuscular Junction of Drosophila Melanogaster

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