Comprehensive Analysis of N6-Methyladenosine (m6A) Methylation in Neuromyelitis Optica Spectrum Disorders

Background: N6-Methyladenosine (m6A) methylation is the most prevalent internal posttranscriptional modification on mammalian mRNA. But its role in neuromyelitis optica spectrum disorders (NMOSD) is not known.Aims: To explore the mechanism of m6A in NMOSD patients.Methods: This study assessed the m6...

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Autores principales:	Hong Yang, Yi-Fan Wu, Jie Ding, Wei Liu, De-Sheng Zhu, Xia-Feng Shen, Yang-Tai Guan
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	neuromyelitis optica spectrum disorder immune homeostasis N6-methyladenosine (m6A) methylation differential methylation peaks MeRIP-seq UPLC-QQQ-MS Genetics QH426-470
Acceso en línea:	https://doaj.org/article/e878a203c000404e831165b23d4609b3
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spelling	oai:doaj.org-article:e878a203c000404e831165b23d4609b32021-11-11T07:43:34ZComprehensive Analysis of N6-Methyladenosine (m6A) Methylation in Neuromyelitis Optica Spectrum Disorders1664-802110.3389/fgene.2021.735454https://doaj.org/article/e878a203c000404e831165b23d4609b32021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.735454/fullhttps://doaj.org/toc/1664-8021Background: N6-Methyladenosine (m6A) methylation is the most prevalent internal posttranscriptional modification on mammalian mRNA. But its role in neuromyelitis optica spectrum disorders (NMOSD) is not known.Aims: To explore the mechanism of m6A in NMOSD patients.Methods: This study assessed the m6A methylation levels in blood from two groups: NMOSD patients and healthy controls. Methylated RNA immunoprecipitation Sequencing (MeRIP-seq) and RNA-seq were performed to assess differences in m6A methylation between NMOSD patients and healthy controls. Ultra-high performance liquid chromatography coupled with triple quadruple mass spectrometry (UPLC-QQQ-MS) method was performed to check m6A level. Differential m6A methylation genes were validated by MeRIP-qPCR.Results: Compared with that in the control group, the total m6A level was decreased in the NMOSD group. Genes with upregulated methylation were primarily enriched in processes associated with RNA splicing, mRNA processing, and innate immune response, while genes with downregulated methylation were enriched in processes associated with the regulation of transcription, DNA-templating, and the positive regulation of I-kappa B kinase/NF-kappa B signalling.Conclusion: These findings demonstrate that differential m6A methylation may act on functional genes to regulate immune homeostasis in NMOSD.Hong YangYi-Fan WuJie DingWei LiuDe-Sheng ZhuXia-Feng ShenYang-Tai GuanYang-Tai GuanFrontiers Media S.A.articleneuromyelitis optica spectrum disorderimmune homeostasisN6-methyladenosine (m6A) methylationdifferential methylation peaksMeRIP-seqUPLC-QQQ-MSGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	neuromyelitis optica spectrum disorder immune homeostasis N6-methyladenosine (m6A) methylation differential methylation peaks MeRIP-seq UPLC-QQQ-MS Genetics QH426-470
spellingShingle	neuromyelitis optica spectrum disorder immune homeostasis N6-methyladenosine (m6A) methylation differential methylation peaks MeRIP-seq UPLC-QQQ-MS Genetics QH426-470 Hong Yang Yi-Fan Wu Jie Ding Wei Liu De-Sheng Zhu Xia-Feng Shen Yang-Tai Guan Yang-Tai Guan Comprehensive Analysis of N6-Methyladenosine (m6A) Methylation in Neuromyelitis Optica Spectrum Disorders
description	Background: N6-Methyladenosine (m6A) methylation is the most prevalent internal posttranscriptional modification on mammalian mRNA. But its role in neuromyelitis optica spectrum disorders (NMOSD) is not known.Aims: To explore the mechanism of m6A in NMOSD patients.Methods: This study assessed the m6A methylation levels in blood from two groups: NMOSD patients and healthy controls. Methylated RNA immunoprecipitation Sequencing (MeRIP-seq) and RNA-seq were performed to assess differences in m6A methylation between NMOSD patients and healthy controls. Ultra-high performance liquid chromatography coupled with triple quadruple mass spectrometry (UPLC-QQQ-MS) method was performed to check m6A level. Differential m6A methylation genes were validated by MeRIP-qPCR.Results: Compared with that in the control group, the total m6A level was decreased in the NMOSD group. Genes with upregulated methylation were primarily enriched in processes associated with RNA splicing, mRNA processing, and innate immune response, while genes with downregulated methylation were enriched in processes associated with the regulation of transcription, DNA-templating, and the positive regulation of I-kappa B kinase/NF-kappa B signalling.Conclusion: These findings demonstrate that differential m6A methylation may act on functional genes to regulate immune homeostasis in NMOSD.
format	article
author	Hong Yang Yi-Fan Wu Jie Ding Wei Liu De-Sheng Zhu Xia-Feng Shen Yang-Tai Guan Yang-Tai Guan
author_facet	Hong Yang Yi-Fan Wu Jie Ding Wei Liu De-Sheng Zhu Xia-Feng Shen Yang-Tai Guan Yang-Tai Guan
author_sort	Hong Yang
title	Comprehensive Analysis of N6-Methyladenosine (m6A) Methylation in Neuromyelitis Optica Spectrum Disorders
title_short	Comprehensive Analysis of N6-Methyladenosine (m6A) Methylation in Neuromyelitis Optica Spectrum Disorders
title_full	Comprehensive Analysis of N6-Methyladenosine (m6A) Methylation in Neuromyelitis Optica Spectrum Disorders
title_fullStr	Comprehensive Analysis of N6-Methyladenosine (m6A) Methylation in Neuromyelitis Optica Spectrum Disorders
title_full_unstemmed	Comprehensive Analysis of N6-Methyladenosine (m6A) Methylation in Neuromyelitis Optica Spectrum Disorders
title_sort	comprehensive analysis of n6-methyladenosine (m6a) methylation in neuromyelitis optica spectrum disorders
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e878a203c000404e831165b23d4609b3
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Comprehensive Analysis of N6-Methyladenosine (m6A) Methylation in Neuromyelitis Optica Spectrum Disorders

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