Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy

Impaired motor and sensory functions are the main features of Charcot–Marie–Tooth disease. Mesenchymal stem cell (MSCs) therapy is one of the possible treatments for this disease. It was assumed that MSCs therapy can improve the contractile properties of the triceps surae (TS) muscles in mice with h...

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Autores principales:	Iryna Govbakh, Vitalii Kyryk, Alina Ustymenko, Volodymyr Rubtsov, Oleg Tsupykov, Nataliya V. Bulgakova, Danylo O. Zavodovskiy, Inna Sokolowska, Andriy Maznychenko
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	stem cells hereditary peripheral neuropathy muscle contraction electrical stimulation mice Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/e87b96d482974781a68334c4c65c0e42
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spelling	oai:doaj.org-article:e87b96d482974781a68334c4c65c0e422021-11-11T17:25:44ZStem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy10.3390/ijms2221120261422-00671661-6596https://doaj.org/article/e87b96d482974781a68334c4c65c0e422021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12026https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Impaired motor and sensory functions are the main features of Charcot–Marie–Tooth disease. Mesenchymal stem cell (MSCs) therapy is one of the possible treatments for this disease. It was assumed that MSCs therapy can improve the contractile properties of the triceps surae (TS) muscles in mice with hereditary peripheral neuropathy. Murine adipose-derived mesenchymal stromal cells (AD-MSCs) were obtained for transplantation into TS muscles of <i>FVB-C-Tg(GFPU)5Nagy/J</i> mice. Three months after AD-MSCs transplantation, animals were subjected to electrophysiological investigations. Parameters of TS muscle tension after intermittent high frequency electrical sciatic nerve stimulations were analyzed. It was found that force of TS muscle tension contraction in animals after AD-MSCs treatment was two-time higher than in untreated mice. Normalized values of force muscle contraction in different phases of electrical stimulation were 0.3 ± 0.01 vs. 0.18 ± 0.01 and 0.26 ± 0.03 vs. 0.13 ± 0.03 for treated and untreated animals, respectively. It is assumed that the two-fold increase in TS muscle strength was caused by stem cell therapy. Apparently, AD-MSCs therapy can promote nerve regeneration and partial restoration of muscle function, and thus can be a potential therapeutic agent for the treatment of peripheral neuropathies.Iryna GovbakhVitalii KyrykAlina UstymenkoVolodymyr RubtsovOleg TsupykovNataliya V. BulgakovaDanylo O. ZavodovskiyInna SokolowskaAndriy MaznychenkoMDPI AGarticlestem cellshereditary peripheral neuropathymuscle contractionelectrical stimulationmiceBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12026, p 12026 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	stem cells hereditary peripheral neuropathy muscle contraction electrical stimulation mice Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	stem cells hereditary peripheral neuropathy muscle contraction electrical stimulation mice Biology (General) QH301-705.5 Chemistry QD1-999 Iryna Govbakh Vitalii Kyryk Alina Ustymenko Volodymyr Rubtsov Oleg Tsupykov Nataliya V. Bulgakova Danylo O. Zavodovskiy Inna Sokolowska Andriy Maznychenko Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy
description	Impaired motor and sensory functions are the main features of Charcot–Marie–Tooth disease. Mesenchymal stem cell (MSCs) therapy is one of the possible treatments for this disease. It was assumed that MSCs therapy can improve the contractile properties of the triceps surae (TS) muscles in mice with hereditary peripheral neuropathy. Murine adipose-derived mesenchymal stromal cells (AD-MSCs) were obtained for transplantation into TS muscles of <i>FVB-C-Tg(GFPU)5Nagy/J</i> mice. Three months after AD-MSCs transplantation, animals were subjected to electrophysiological investigations. Parameters of TS muscle tension after intermittent high frequency electrical sciatic nerve stimulations were analyzed. It was found that force of TS muscle tension contraction in animals after AD-MSCs treatment was two-time higher than in untreated mice. Normalized values of force muscle contraction in different phases of electrical stimulation were 0.3 ± 0.01 vs. 0.18 ± 0.01 and 0.26 ± 0.03 vs. 0.13 ± 0.03 for treated and untreated animals, respectively. It is assumed that the two-fold increase in TS muscle strength was caused by stem cell therapy. Apparently, AD-MSCs therapy can promote nerve regeneration and partial restoration of muscle function, and thus can be a potential therapeutic agent for the treatment of peripheral neuropathies.
format	article
author	Iryna Govbakh Vitalii Kyryk Alina Ustymenko Volodymyr Rubtsov Oleg Tsupykov Nataliya V. Bulgakova Danylo O. Zavodovskiy Inna Sokolowska Andriy Maznychenko
author_facet	Iryna Govbakh Vitalii Kyryk Alina Ustymenko Volodymyr Rubtsov Oleg Tsupykov Nataliya V. Bulgakova Danylo O. Zavodovskiy Inna Sokolowska Andriy Maznychenko
author_sort	Iryna Govbakh
title	Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy
title_short	Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy
title_full	Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy
title_fullStr	Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy
title_full_unstemmed	Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy
title_sort	stem cell therapy enhances motor activity of triceps surae muscle in mice with hereditary peripheral neuropathy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e87b96d482974781a68334c4c65c0e42
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Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy

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