Development of Normalized Cdna Library from Fusarium Wilt Infected Roots of a Tolerant Banana Genotype 'Calcutta-4' Musa acuminata ssp. burmannicoides

Management of the most devasting disease, Fusarium wilt of banana, caused by the fungus Fusarium oxysporum f. sp., cubense, is a challenge to the plant pathologist and the banana grower. Currently, genomics is providing the way for understanding plant defense mechanism, having acquired an important...

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Autores principales:	V Swarupa, A Rekha, K V Ravishankar
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2014
Materias:	fusarium oxysporum f.sp. cubense banana cdna library defense response normalization Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/e89c7281d2dd48d4a7f34bdd04419863
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spelling	oai:doaj.org-article:e89c7281d2dd48d4a7f34bdd044198632021-12-02T11:12:39ZDevelopment of Normalized Cdna Library from Fusarium Wilt Infected Roots of a Tolerant Banana Genotype 'Calcutta-4' Musa acuminata ssp. burmannicoides0973-354X2582-4899https://doaj.org/article/e89c7281d2dd48d4a7f34bdd044198632014-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/223https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Management of the most devasting disease, Fusarium wilt of banana, caused by the fungus Fusarium oxysporum f. sp., cubense, is a challenge to the plant pathologist and the banana grower. Currently, genomics is providing the way for understanding plant defense mechanism, having acquired an important place in crop improvement. To identify the relevant genes and to understand the defense mechanism induced during Fusarium wilt infection, a normalized cDNA library was constructed from infected root samples of a tolerant banana genotype, Musa acuminata spp. burmannicoides 'Calcutta-4', by duplex specific nuclease (DSN) based normalization, using the SMART (switching mechanism at 5' end of RNA transcript) full-length cDNA construction method. Sequencing and analysis of 600 clones revealed 392 non-redundant clones. In all, of 88% of the sequences were annotated using Musa genome database, and the remaining 12% were identified as novel loci not annotated. We observed several resistance genes, ROS scavenging genes and genes involved in ubiquitin-proteosome pathway in this study. These genes may have a possible role against Foc infection. These sequences would enrich the EST data developed against specific stress, which is an indispensable tool for predicting functional genes and understanding the defense mechanism.V SwarupaA RekhaK V RavishankarSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlefusarium oxysporum f.sp. cubensebananacdna librarydefense responsenormalizationPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 9, Iss 1, Pp 55-60 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	fusarium oxysporum f.sp. cubense banana cdna library defense response normalization Plant culture SB1-1110
spellingShingle	fusarium oxysporum f.sp. cubense banana cdna library defense response normalization Plant culture SB1-1110 V Swarupa A Rekha K V Ravishankar Development of Normalized Cdna Library from Fusarium Wilt Infected Roots of a Tolerant Banana Genotype 'Calcutta-4' Musa acuminata ssp. burmannicoides
description	Management of the most devasting disease, Fusarium wilt of banana, caused by the fungus Fusarium oxysporum f. sp., cubense, is a challenge to the plant pathologist and the banana grower. Currently, genomics is providing the way for understanding plant defense mechanism, having acquired an important place in crop improvement. To identify the relevant genes and to understand the defense mechanism induced during Fusarium wilt infection, a normalized cDNA library was constructed from infected root samples of a tolerant banana genotype, Musa acuminata spp. burmannicoides 'Calcutta-4', by duplex specific nuclease (DSN) based normalization, using the SMART (switching mechanism at 5' end of RNA transcript) full-length cDNA construction method. Sequencing and analysis of 600 clones revealed 392 non-redundant clones. In all, of 88% of the sequences were annotated using Musa genome database, and the remaining 12% were identified as novel loci not annotated. We observed several resistance genes, ROS scavenging genes and genes involved in ubiquitin-proteosome pathway in this study. These genes may have a possible role against Foc infection. These sequences would enrich the EST data developed against specific stress, which is an indispensable tool for predicting functional genes and understanding the defense mechanism.
format	article
author	V Swarupa A Rekha K V Ravishankar
author_facet	V Swarupa A Rekha K V Ravishankar
author_sort	V Swarupa
title	Development of Normalized Cdna Library from Fusarium Wilt Infected Roots of a Tolerant Banana Genotype 'Calcutta-4' Musa acuminata ssp. burmannicoides
title_short	Development of Normalized Cdna Library from Fusarium Wilt Infected Roots of a Tolerant Banana Genotype 'Calcutta-4' Musa acuminata ssp. burmannicoides
title_full	Development of Normalized Cdna Library from Fusarium Wilt Infected Roots of a Tolerant Banana Genotype 'Calcutta-4' Musa acuminata ssp. burmannicoides
title_fullStr	Development of Normalized Cdna Library from Fusarium Wilt Infected Roots of a Tolerant Banana Genotype 'Calcutta-4' Musa acuminata ssp. burmannicoides
title_full_unstemmed	Development of Normalized Cdna Library from Fusarium Wilt Infected Roots of a Tolerant Banana Genotype 'Calcutta-4' Musa acuminata ssp. burmannicoides
title_sort	development of normalized cdna library from fusarium wilt infected roots of a tolerant banana genotype 'calcutta-4' musa acuminata ssp. burmannicoides
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2014
url	https://doaj.org/article/e89c7281d2dd48d4a7f34bdd04419863
work_keys_str_mv	AT vswarupa developmentofnormalizedcdnalibraryfromfusariumwiltinfectedrootsofatolerantbananagenotypecalcutta4musaacuminatasspburmannicoides AT arekha developmentofnormalizedcdnalibraryfromfusariumwiltinfectedrootsofatolerantbananagenotypecalcutta4musaacuminatasspburmannicoides AT kvravishankar developmentofnormalizedcdnalibraryfromfusariumwiltinfectedrootsofatolerantbananagenotypecalcutta4musaacuminatasspburmannicoides
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Development of Normalized Cdna Library from Fusarium Wilt Infected Roots of a Tolerant Banana Genotype 'Calcutta-4' Musa acuminata ssp. burmannicoides

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