The CCAAT-Binding Complex Controls Respiratory Gene Expression and Iron Homeostasis in Candida Glabrata

Abstract The CCAAT-binding complex (CBC) is a heterotrimeric transcription factor which is widely conserved in eukaryotes. In the model yeast S. cerevisiae, CBC positively controls the expression of respiratory pathway genes. This role involves interactions with the regulatory subunit Hap4. In many...

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Autores principales: Antonin Thiébaut, Thierry Delaveau, Médine Benchouaia, Julia Boeri, Mathilde Garcia, Gaëlle Lelandais, Frédéric Devaux
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Publicado: Nature Portfolio 2017
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spelling oai:doaj.org-article:e89e53f32293430fa3659c0cd66b831d2021-12-02T11:41:00ZThe CCAAT-Binding Complex Controls Respiratory Gene Expression and Iron Homeostasis in Candida Glabrata10.1038/s41598-017-03750-52045-2322https://doaj.org/article/e89e53f32293430fa3659c0cd66b831d2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03750-5https://doaj.org/toc/2045-2322Abstract The CCAAT-binding complex (CBC) is a heterotrimeric transcription factor which is widely conserved in eukaryotes. In the model yeast S. cerevisiae, CBC positively controls the expression of respiratory pathway genes. This role involves interactions with the regulatory subunit Hap4. In many pathogenic fungi, CBC interacts with the HapX regulatory subunit to control iron homeostasis. HapX is a bZIP protein which only shares with Hap4 the Hap4Like domain (Hap4L) required for its interaction with CBC. Here, we show that CBC has a dual role in the pathogenic yeast C. glabrata. It is required, along with Hap4, for the constitutive expression of respiratory genes and it is also essential for the iron stress response, which is mediated by the Yap5 bZIP transcription factor. Interestingly, Yap5 contains a vestigial Hap4L domain. The mutagenesis of this domain severely reduced Yap5 binding to its targets and compromised its interaction with Hap5. Hence, Yap5, like HapX in other species, acts as a CBC regulatory subunit in the regulation of iron stress response. This work reveals new aspects of iron homeostasis in C. glabrata and of the evolution of the role of CBC and Hap4L-bZIP proteins in this process.Antonin ThiébautThierry DelaveauMédine BenchouaiaJulia BoeriMathilde GarciaGaëlle LelandaisFrédéric DevauxNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Antonin Thiébaut
Thierry Delaveau
Médine Benchouaia
Julia Boeri
Mathilde Garcia
Gaëlle Lelandais
Frédéric Devaux
The CCAAT-Binding Complex Controls Respiratory Gene Expression and Iron Homeostasis in Candida Glabrata
description Abstract The CCAAT-binding complex (CBC) is a heterotrimeric transcription factor which is widely conserved in eukaryotes. In the model yeast S. cerevisiae, CBC positively controls the expression of respiratory pathway genes. This role involves interactions with the regulatory subunit Hap4. In many pathogenic fungi, CBC interacts with the HapX regulatory subunit to control iron homeostasis. HapX is a bZIP protein which only shares with Hap4 the Hap4Like domain (Hap4L) required for its interaction with CBC. Here, we show that CBC has a dual role in the pathogenic yeast C. glabrata. It is required, along with Hap4, for the constitutive expression of respiratory genes and it is also essential for the iron stress response, which is mediated by the Yap5 bZIP transcription factor. Interestingly, Yap5 contains a vestigial Hap4L domain. The mutagenesis of this domain severely reduced Yap5 binding to its targets and compromised its interaction with Hap5. Hence, Yap5, like HapX in other species, acts as a CBC regulatory subunit in the regulation of iron stress response. This work reveals new aspects of iron homeostasis in C. glabrata and of the evolution of the role of CBC and Hap4L-bZIP proteins in this process.
format article
author Antonin Thiébaut
Thierry Delaveau
Médine Benchouaia
Julia Boeri
Mathilde Garcia
Gaëlle Lelandais
Frédéric Devaux
author_facet Antonin Thiébaut
Thierry Delaveau
Médine Benchouaia
Julia Boeri
Mathilde Garcia
Gaëlle Lelandais
Frédéric Devaux
author_sort Antonin Thiébaut
title The CCAAT-Binding Complex Controls Respiratory Gene Expression and Iron Homeostasis in Candida Glabrata
title_short The CCAAT-Binding Complex Controls Respiratory Gene Expression and Iron Homeostasis in Candida Glabrata
title_full The CCAAT-Binding Complex Controls Respiratory Gene Expression and Iron Homeostasis in Candida Glabrata
title_fullStr The CCAAT-Binding Complex Controls Respiratory Gene Expression and Iron Homeostasis in Candida Glabrata
title_full_unstemmed The CCAAT-Binding Complex Controls Respiratory Gene Expression and Iron Homeostasis in Candida Glabrata
title_sort ccaat-binding complex controls respiratory gene expression and iron homeostasis in candida glabrata
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/e89e53f32293430fa3659c0cd66b831d
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