Photocontrolled apoptosis induction using precursor miR-664a and an RNA carrier-conjugated with photosensitizer

Abstract Methods to spatially induce apoptosis are useful for cancer therapy. To control the induction of apoptosis, methods using light, such as photochemical internalization (PCI), have been developed. We hypothesized that photoinduced delivery of microRNAs (miRNAs) that regulate apoptosis could s...

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Autores principales:	Kazunori Watanabe, Tomoko Nawachi, Ruriko Okutani, Takashi Ohtsuki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e89e738ac0294ecab0e853010feb3939
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spelling	oai:doaj.org-article:e89e738ac0294ecab0e853010feb39392021-12-02T17:56:56ZPhotocontrolled apoptosis induction using precursor miR-664a and an RNA carrier-conjugated with photosensitizer10.1038/s41598-021-94249-72045-2322https://doaj.org/article/e89e738ac0294ecab0e853010feb39392021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94249-7https://doaj.org/toc/2045-2322Abstract Methods to spatially induce apoptosis are useful for cancer therapy. To control the induction of apoptosis, methods using light, such as photochemical internalization (PCI), have been developed. We hypothesized that photoinduced delivery of microRNAs (miRNAs) that regulate apoptosis could spatially induce apoptosis. In this study, we identified pre-miR-664a as a novel apoptosis-inducing miRNA via mitochondrial apoptotic pathway. Further, we demonstrated the utility of photoinduced cytosolic dispersion of RNA (PCDR), which is an intracellular RNA delivery method based on PCI. Indeed, apoptosis is spatially regulated by pre-miR-664a and PCDR. In addition, we found that apoptosis induced by pre-miR-664a delivered by PCDR was more rapid than that by lipofection. These results suggest that pre-miR-664a is a nucleic acid drug candidate for cancer therapy and PCDR and pre-miR-664a-based strategies have potential therapeutic uses for diseases affecting various cell types.Kazunori WatanabeTomoko NawachiRuriko OkutaniTakashi OhtsukiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kazunori Watanabe Tomoko Nawachi Ruriko Okutani Takashi Ohtsuki Photocontrolled apoptosis induction using precursor miR-664a and an RNA carrier-conjugated with photosensitizer
description	Abstract Methods to spatially induce apoptosis are useful for cancer therapy. To control the induction of apoptosis, methods using light, such as photochemical internalization (PCI), have been developed. We hypothesized that photoinduced delivery of microRNAs (miRNAs) that regulate apoptosis could spatially induce apoptosis. In this study, we identified pre-miR-664a as a novel apoptosis-inducing miRNA via mitochondrial apoptotic pathway. Further, we demonstrated the utility of photoinduced cytosolic dispersion of RNA (PCDR), which is an intracellular RNA delivery method based on PCI. Indeed, apoptosis is spatially regulated by pre-miR-664a and PCDR. In addition, we found that apoptosis induced by pre-miR-664a delivered by PCDR was more rapid than that by lipofection. These results suggest that pre-miR-664a is a nucleic acid drug candidate for cancer therapy and PCDR and pre-miR-664a-based strategies have potential therapeutic uses for diseases affecting various cell types.
format	article
author	Kazunori Watanabe Tomoko Nawachi Ruriko Okutani Takashi Ohtsuki
author_facet	Kazunori Watanabe Tomoko Nawachi Ruriko Okutani Takashi Ohtsuki
author_sort	Kazunori Watanabe
title	Photocontrolled apoptosis induction using precursor miR-664a and an RNA carrier-conjugated with photosensitizer
title_short	Photocontrolled apoptosis induction using precursor miR-664a and an RNA carrier-conjugated with photosensitizer
title_full	Photocontrolled apoptosis induction using precursor miR-664a and an RNA carrier-conjugated with photosensitizer
title_fullStr	Photocontrolled apoptosis induction using precursor miR-664a and an RNA carrier-conjugated with photosensitizer
title_full_unstemmed	Photocontrolled apoptosis induction using precursor miR-664a and an RNA carrier-conjugated with photosensitizer
title_sort	photocontrolled apoptosis induction using precursor mir-664a and an rna carrier-conjugated with photosensitizer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e89e738ac0294ecab0e853010feb3939
work_keys_str_mv	AT kazunoriwatanabe photocontrolledapoptosisinductionusingprecursormir664aandanrnacarrierconjugatedwithphotosensitizer AT tomokonawachi photocontrolledapoptosisinductionusingprecursormir664aandanrnacarrierconjugatedwithphotosensitizer AT rurikookutani photocontrolledapoptosisinductionusingprecursormir664aandanrnacarrierconjugatedwithphotosensitizer AT takashiohtsuki photocontrolledapoptosisinductionusingprecursormir664aandanrnacarrierconjugatedwithphotosensitizer
_version_	1718379081012084736

Photocontrolled apoptosis induction using precursor miR-664a and an RNA carrier-conjugated with photosensitizer

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