Clinical and biochemical study of D-serine metabolism among schizophrenia patients

Hamdy N El-Tallawy,1 Tahia H Saleem,2 Abdallah MAA El-Ebidi,3 Mohammed H Hassan,4 Romany H Gabra,1 Wafaa MA Farghaly,1 Nagwa Abo El-Maali,5 Hoda S Sherkawy3 1Department of Neuropsychiatry, 2Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Assiut University, Assiut, 3De...

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Autores principales:	El-Tallawy HN, Saleem TH, El-Ebidi AMA, Hassan MH, Gabra RH, Farghaly WMA, Abo El-Maali N, Sherkawy HS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	D-Serine Serine racemase D- amino acid oxidase Schizophrenia Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/e89f468f4b674bd5b694559557607bbc
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spelling	oai:doaj.org-article:e89f468f4b674bd5b694559557607bbc2021-12-02T06:48:12ZClinical and biochemical study of D-serine metabolism among schizophrenia patients1178-2021https://doaj.org/article/e89f468f4b674bd5b694559557607bbc2017-04-01T00:00:00Zhttps://www.dovepress.com/clinical-and-biochemical-study-of-d-serine-metabolism-among-schizophre-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Hamdy N El-Tallawy,1 Tahia H Saleem,2 Abdallah MAA El-Ebidi,3 Mohammed H Hassan,4 Romany H Gabra,1 Wafaa MA Farghaly,1 Nagwa Abo El-Maali,5 Hoda S Sherkawy3 1Department of Neuropsychiatry, 2Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Assiut University, Assiut, 3Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Aswan University, Aswan, 4Department of Medical Biochemistry and Molecular Biology, Qena Faculty of Medicine, South Valley University, Qena, 5Department of Chemistry, Faculty of Science, Assiut University, Assiut, Egypt Background: Schizophrenia is a typical N-methyl-d-aspartate receptor (NMDA-R) hypofunction disorder. Decreased d-serine (d-Ser) levels in the periphery occur in schizophrenia and may reflect decreased availability of d-Ser to activate NMDA-R in the brain.Objective: The objective of this study was to investigate the role of d-Ser metabolism in the pathogenesis of schizophrenia via biochemical assays and correlates, the serum level of d-Ser, d-serine racemase (SR) (responsible for its formation from l-serine [l-Ser]) and d-amino acid oxidase (DAAO) (responsible for its catabolism), among different clinical types of schizophrenia patients.Patients and methods: This cross-sectional case–control study was carried out on 100 patients and 50 controls. They were recruited from the outpatients’ psychiatric unit of the Neuropsychiatric Department of Assiut University Hospital, Upper Egypt. The type of schizophrenia was determined according to the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV), while the severity of schizophrenia was determined according to the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5). Serum d-Ser levels were estimated using high-performance liquid chromatography (HPLC), while serum SR and DAAO were measured using commercially available enzyme-linked immunosorbent assay kits.Results: There were significantly lower mean serum levels of d-Ser and SR and significantly higher mean serum levels of DAAO (P-value <0.01 for each) among schizophrenia patients when compared with the control group. Paranoid schizophrenia had the highest frequency, with a significantly lower serum levels of d-Ser and SR in the residual type and significantly higher serum levels of DAAO in undifferentiated and catatonic types. Combined receiver-operating characteristic curve for serum d-Ser, SR and DAAO indicated that the best serum level cutoff points at which schizophrenia manifestations started to appear were ≤61.4 mg/L for d-Ser, ≤15.5 pg/mL for SR and >35.6 pg/mL for DAAO.Conclusion: The present study confirms that disturbed d-Ser metabolism could be implicated in the pathogenesis of schizophrenia. Keywords: d-serine, serine racemase, d-amino acid oxidase, schizophrenia, HPLCEl-Tallawy HNSaleem THEl-Ebidi AMAHassan MHGabra RHFarghaly WMAAbo El-Maali NSherkawy HSDove Medical PressarticleD-SerineSerine racemaseD- amino acid oxidaseSchizophreniaNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 13, Pp 1057-1063 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	D-Serine Serine racemase D- amino acid oxidase Schizophrenia Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	D-Serine Serine racemase D- amino acid oxidase Schizophrenia Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 El-Tallawy HN Saleem TH El-Ebidi AMA Hassan MH Gabra RH Farghaly WMA Abo El-Maali N Sherkawy HS Clinical and biochemical study of D-serine metabolism among schizophrenia patients
description	Hamdy N El-Tallawy,1 Tahia H Saleem,2 Abdallah MAA El-Ebidi,3 Mohammed H Hassan,4 Romany H Gabra,1 Wafaa MA Farghaly,1 Nagwa Abo El-Maali,5 Hoda S Sherkawy3 1Department of Neuropsychiatry, 2Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Assiut University, Assiut, 3Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Aswan University, Aswan, 4Department of Medical Biochemistry and Molecular Biology, Qena Faculty of Medicine, South Valley University, Qena, 5Department of Chemistry, Faculty of Science, Assiut University, Assiut, Egypt Background: Schizophrenia is a typical N-methyl-d-aspartate receptor (NMDA-R) hypofunction disorder. Decreased d-serine (d-Ser) levels in the periphery occur in schizophrenia and may reflect decreased availability of d-Ser to activate NMDA-R in the brain.Objective: The objective of this study was to investigate the role of d-Ser metabolism in the pathogenesis of schizophrenia via biochemical assays and correlates, the serum level of d-Ser, d-serine racemase (SR) (responsible for its formation from l-serine [l-Ser]) and d-amino acid oxidase (DAAO) (responsible for its catabolism), among different clinical types of schizophrenia patients.Patients and methods: This cross-sectional case–control study was carried out on 100 patients and 50 controls. They were recruited from the outpatients’ psychiatric unit of the Neuropsychiatric Department of Assiut University Hospital, Upper Egypt. The type of schizophrenia was determined according to the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV), while the severity of schizophrenia was determined according to the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5). Serum d-Ser levels were estimated using high-performance liquid chromatography (HPLC), while serum SR and DAAO were measured using commercially available enzyme-linked immunosorbent assay kits.Results: There were significantly lower mean serum levels of d-Ser and SR and significantly higher mean serum levels of DAAO (P-value <0.01 for each) among schizophrenia patients when compared with the control group. Paranoid schizophrenia had the highest frequency, with a significantly lower serum levels of d-Ser and SR in the residual type and significantly higher serum levels of DAAO in undifferentiated and catatonic types. Combined receiver-operating characteristic curve for serum d-Ser, SR and DAAO indicated that the best serum level cutoff points at which schizophrenia manifestations started to appear were ≤61.4 mg/L for d-Ser, ≤15.5 pg/mL for SR and >35.6 pg/mL for DAAO.Conclusion: The present study confirms that disturbed d-Ser metabolism could be implicated in the pathogenesis of schizophrenia. Keywords: d-serine, serine racemase, d-amino acid oxidase, schizophrenia, HPLC
format	article
author	El-Tallawy HN Saleem TH El-Ebidi AMA Hassan MH Gabra RH Farghaly WMA Abo El-Maali N Sherkawy HS
author_facet	El-Tallawy HN Saleem TH El-Ebidi AMA Hassan MH Gabra RH Farghaly WMA Abo El-Maali N Sherkawy HS
author_sort	El-Tallawy HN
title	Clinical and biochemical study of D-serine metabolism among schizophrenia patients
title_short	Clinical and biochemical study of D-serine metabolism among schizophrenia patients
title_full	Clinical and biochemical study of D-serine metabolism among schizophrenia patients
title_fullStr	Clinical and biochemical study of D-serine metabolism among schizophrenia patients
title_full_unstemmed	Clinical and biochemical study of D-serine metabolism among schizophrenia patients
title_sort	clinical and biochemical study of d-serine metabolism among schizophrenia patients
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/e89f468f4b674bd5b694559557607bbc
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_version_	1718399786627891200

Clinical and biochemical study of D-serine metabolism among schizophrenia patients

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