Silencing mutated β-catenin inhibits cell proliferation and stimulates apoptosis in the adrenocortical cancer cell line H295R.
<h4>Context</h4>Adrenocortical carcinoma (ACC) is a rare and highly aggressive endocrine neoplasm, with limited therapeutic options. Activating β-catenin somatic mutations are found in ACC and have been associated with a poor clinical outcome. In fact, activation of the Wnt/β-catenin sig...
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2013
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oai:doaj.org-article:e8baa0d2cca04cffbfe551e33e213bea2021-11-18T07:58:20ZSilencing mutated β-catenin inhibits cell proliferation and stimulates apoptosis in the adrenocortical cancer cell line H295R.1932-620310.1371/journal.pone.0055743https://doaj.org/article/e8baa0d2cca04cffbfe551e33e213bea2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23409032/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Context</h4>Adrenocortical carcinoma (ACC) is a rare and highly aggressive endocrine neoplasm, with limited therapeutic options. Activating β-catenin somatic mutations are found in ACC and have been associated with a poor clinical outcome. In fact, activation of the Wnt/β-catenin signaling pathway seems to play a major role in ACC aggressiveness, and might, thus, represent a promising therapeutic target.<h4>Objective</h4>Similar to patient tumor specimen the H295 cell line derived from an ACC harbors a natural activating β-catenin mutation. We herein assess the in vitro and in vivo effect of β-catenin inactivation using a doxycyclin (dox) inducible shRNA plasmid in H295R adrenocortical cancer cells line (clone named shβ).<h4>Results</h4>Following dox treatment a profound reduction in β-catenin expression was detectable in shβ clones in comparison to control clones (Ctr). Accordingly, we observed a decrease in Wnt/βcatenin-dependent luciferase reporter activity as well as a decreased expression of AXIN2 representing an endogenous β-catenin target gene. Concomitantly, β-catenin silencing resulted in a decreased cell proliferation, cell cycle alterations with cell accumulation in the G1 phase and increased apoptosis in vitro. In vivo, on established tumor xenografts in athymic nude mice, 9 days of β-catenin silencing resulted in a significant reduction of CTNNB1 and AXIN2 expression. Moreover, continous β-catenin silencing, starting 3 days after tumor cell inoculation, was associated with a complete absence of tumor growth in the shβ group while tumors were present in all animals of the control group.<h4>Conclusion</h4>In summary, these experiments provide evidences that Wnt/β-catenin pathway inhibition in ACC is a promising therapeutic target.Sébastien GaujouxConstanze HantelPierre LaunayStéphane BonnetKarine PerlemoineLucile LefèvreMarine Guillaud-BatailleFelix BeuschleinFrédérique TissierJérôme BertheratMarthe Rizk-RabinBruno RagazzonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e55743 (2013) |
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Medicine R Science Q Sébastien Gaujoux Constanze Hantel Pierre Launay Stéphane Bonnet Karine Perlemoine Lucile Lefèvre Marine Guillaud-Bataille Felix Beuschlein Frédérique Tissier Jérôme Bertherat Marthe Rizk-Rabin Bruno Ragazzon Silencing mutated β-catenin inhibits cell proliferation and stimulates apoptosis in the adrenocortical cancer cell line H295R. |
| description |
<h4>Context</h4>Adrenocortical carcinoma (ACC) is a rare and highly aggressive endocrine neoplasm, with limited therapeutic options. Activating β-catenin somatic mutations are found in ACC and have been associated with a poor clinical outcome. In fact, activation of the Wnt/β-catenin signaling pathway seems to play a major role in ACC aggressiveness, and might, thus, represent a promising therapeutic target.<h4>Objective</h4>Similar to patient tumor specimen the H295 cell line derived from an ACC harbors a natural activating β-catenin mutation. We herein assess the in vitro and in vivo effect of β-catenin inactivation using a doxycyclin (dox) inducible shRNA plasmid in H295R adrenocortical cancer cells line (clone named shβ).<h4>Results</h4>Following dox treatment a profound reduction in β-catenin expression was detectable in shβ clones in comparison to control clones (Ctr). Accordingly, we observed a decrease in Wnt/βcatenin-dependent luciferase reporter activity as well as a decreased expression of AXIN2 representing an endogenous β-catenin target gene. Concomitantly, β-catenin silencing resulted in a decreased cell proliferation, cell cycle alterations with cell accumulation in the G1 phase and increased apoptosis in vitro. In vivo, on established tumor xenografts in athymic nude mice, 9 days of β-catenin silencing resulted in a significant reduction of CTNNB1 and AXIN2 expression. Moreover, continous β-catenin silencing, starting 3 days after tumor cell inoculation, was associated with a complete absence of tumor growth in the shβ group while tumors were present in all animals of the control group.<h4>Conclusion</h4>In summary, these experiments provide evidences that Wnt/β-catenin pathway inhibition in ACC is a promising therapeutic target. |
| format |
article |
| author |
Sébastien Gaujoux Constanze Hantel Pierre Launay Stéphane Bonnet Karine Perlemoine Lucile Lefèvre Marine Guillaud-Bataille Felix Beuschlein Frédérique Tissier Jérôme Bertherat Marthe Rizk-Rabin Bruno Ragazzon |
| author_facet |
Sébastien Gaujoux Constanze Hantel Pierre Launay Stéphane Bonnet Karine Perlemoine Lucile Lefèvre Marine Guillaud-Bataille Felix Beuschlein Frédérique Tissier Jérôme Bertherat Marthe Rizk-Rabin Bruno Ragazzon |
| author_sort |
Sébastien Gaujoux |
| title |
Silencing mutated β-catenin inhibits cell proliferation and stimulates apoptosis in the adrenocortical cancer cell line H295R. |
| title_short |
Silencing mutated β-catenin inhibits cell proliferation and stimulates apoptosis in the adrenocortical cancer cell line H295R. |
| title_full |
Silencing mutated β-catenin inhibits cell proliferation and stimulates apoptosis in the adrenocortical cancer cell line H295R. |
| title_fullStr |
Silencing mutated β-catenin inhibits cell proliferation and stimulates apoptosis in the adrenocortical cancer cell line H295R. |
| title_full_unstemmed |
Silencing mutated β-catenin inhibits cell proliferation and stimulates apoptosis in the adrenocortical cancer cell line H295R. |
| title_sort |
silencing mutated β-catenin inhibits cell proliferation and stimulates apoptosis in the adrenocortical cancer cell line h295r. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/e8baa0d2cca04cffbfe551e33e213bea |
| work_keys_str_mv |
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