Assessment of Plant Growth Regulators and Carbon Sources on the Germination and Growth Process of Dandelion (Taraxacum officinale G.H. Weber ex Wiggers) under In Vitro Conditions

There are several studies on the medicinal properties of dandelions (Taraxacum officinale), but few studies are aimed at understanding the in vitro germination process of this plant to improve its propagation. This research was focused on studying in vi...

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Autores principales:	María Eugenia Martínez, Lorena Jorquera, Paola Poirrier, Katy Díaz, Rolando Chamy
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dandelion final germination percent germination synchrony <i>in vitro</i> germination seedling vigor index uniformity of germination Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/e8d4e79045394e64a5d44d270e705ade
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spelling	oai:doaj.org-article:e8d4e79045394e64a5d44d270e705ade2021-11-25T17:47:34ZAssessment of Plant Growth Regulators and Carbon Sources on the Germination and Growth Process of Dandelion (<i>Taraxacum officinale</i> G.H. Weber ex Wiggers) under <i>In Vitro</i> Conditions10.3390/horticulturae71104862311-7524https://doaj.org/article/e8d4e79045394e64a5d44d270e705ade2021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/486https://doaj.org/toc/2311-7524There are several studies on the medicinal properties of dandelions (<i>Taraxacum officinale</i>), but few studies are aimed at understanding the <i>in vitro</i> germination process of this plant to improve its propagation. This research was focused on studying <i>in vitro</i> seed germination and development of seedlings under different carbon sources, glucose (GLU) or sucrose (SUC) and its concentrations (1.0–5.5%). Additionally, the effect of supplementation with plant growth regulators (PGRs) was studied, measuring the germination capacity, uncertainty and synchrony. Germination was promoted under low carbon source concentrations (≤2.3%), whereas higher concentrations (≥3.2%) had a detrimental effect on this process. GLU allowed the final germination percentages to be slightly better than SUC. Uniformity and synchrony values improved with the presence of PGRs. Results suggested that the best condition to assess <i>T. officinale</i> seed germination is in a medium containing GLU 2.3%, 0.5 mg/L 1-Naphthaleneacetic acid (NAA) and 0.5 mg/L 6-Benzylaminopurine acid (BAP). After germination, the best condition for optimal growth of <i>T. officinale</i> seedlings was 1.0% SUC supplemented with 0.225 mg/L of NAA and 3.0 mg/L of BAP for initial shoot development. The survival rate was 97% after greenhouse acclimatization. This new method of germination was implemented for the massive propagation of <i>T. officinale</i> for further medicinal studies.María Eugenia MartínezLorena JorqueraPaola PoirrierKaty DíazRolando ChamyMDPI AGarticledandelionfinal germination percentgermination synchrony<i>in vitro</i> germinationseedling vigor indexuniformity of germinationPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 486, p 486 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dandelion final germination percent germination synchrony <i>in vitro</i> germination seedling vigor index uniformity of germination Plant culture SB1-1110
spellingShingle	dandelion final germination percent germination synchrony <i>in vitro</i> germination seedling vigor index uniformity of germination Plant culture SB1-1110 María Eugenia Martínez Lorena Jorquera Paola Poirrier Katy Díaz Rolando Chamy Assessment of Plant Growth Regulators and Carbon Sources on the Germination and Growth Process of Dandelion (<i>Taraxacum officinale</i> G.H. Weber ex Wiggers) under <i>In Vitro</i> Conditions
description	There are several studies on the medicinal properties of dandelions (<i>Taraxacum officinale</i>), but few studies are aimed at understanding the <i>in vitro</i> germination process of this plant to improve its propagation. This research was focused on studying <i>in vitro</i> seed germination and development of seedlings under different carbon sources, glucose (GLU) or sucrose (SUC) and its concentrations (1.0–5.5%). Additionally, the effect of supplementation with plant growth regulators (PGRs) was studied, measuring the germination capacity, uncertainty and synchrony. Germination was promoted under low carbon source concentrations (≤2.3%), whereas higher concentrations (≥3.2%) had a detrimental effect on this process. GLU allowed the final germination percentages to be slightly better than SUC. Uniformity and synchrony values improved with the presence of PGRs. Results suggested that the best condition to assess <i>T. officinale</i> seed germination is in a medium containing GLU 2.3%, 0.5 mg/L 1-Naphthaleneacetic acid (NAA) and 0.5 mg/L 6-Benzylaminopurine acid (BAP). After germination, the best condition for optimal growth of <i>T. officinale</i> seedlings was 1.0% SUC supplemented with 0.225 mg/L of NAA and 3.0 mg/L of BAP for initial shoot development. The survival rate was 97% after greenhouse acclimatization. This new method of germination was implemented for the massive propagation of <i>T. officinale</i> for further medicinal studies.
format	article
author	María Eugenia Martínez Lorena Jorquera Paola Poirrier Katy Díaz Rolando Chamy
author_facet	María Eugenia Martínez Lorena Jorquera Paola Poirrier Katy Díaz Rolando Chamy
author_sort	María Eugenia Martínez
title	Assessment of Plant Growth Regulators and Carbon Sources on the Germination and Growth Process of Dandelion (<i>Taraxacum officinale</i> G.H. Weber ex Wiggers) under <i>In Vitro</i> Conditions
title_short	Assessment of Plant Growth Regulators and Carbon Sources on the Germination and Growth Process of Dandelion (<i>Taraxacum officinale</i> G.H. Weber ex Wiggers) under <i>In Vitro</i> Conditions
title_full	Assessment of Plant Growth Regulators and Carbon Sources on the Germination and Growth Process of Dandelion (<i>Taraxacum officinale</i> G.H. Weber ex Wiggers) under <i>In Vitro</i> Conditions
title_fullStr	Assessment of Plant Growth Regulators and Carbon Sources on the Germination and Growth Process of Dandelion (<i>Taraxacum officinale</i> G.H. Weber ex Wiggers) under <i>In Vitro</i> Conditions
title_full_unstemmed	Assessment of Plant Growth Regulators and Carbon Sources on the Germination and Growth Process of Dandelion (<i>Taraxacum officinale</i> G.H. Weber ex Wiggers) under <i>In Vitro</i> Conditions
title_sort	assessment of plant growth regulators and carbon sources on the germination and growth process of dandelion (<i>taraxacum officinale</i> g.h. weber ex wiggers) under <i>in vitro</i> conditions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e8d4e79045394e64a5d44d270e705ade
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Assessment of Plant Growth Regulators and Carbon Sources on the Germination and Growth Process of Dandelion (<i>Taraxacum officinale</i> G.H. Weber ex Wiggers) under <i>In Vitro</i> Conditions

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